Buffers

Buffers are essential in many different biological and biotechnical applications. GoldBio’s vast array of application-specific buffers include biological, electrophoretic and lysis buffers in order to ensure that we have exactly the right product for both your research needs as well as your budget.

Showing 1-20 of 37 items.

Imidazole, Recrystallized

Catalog ID Size Pricing
I-902-25 25 g $ 68.00
I-902-50 50 g $ 115.00
I-902-100 100 g $ 184.00
I-902-500 500 g $ 562.00

Description

Imidazole is a planar 5-membered ring. It is a highly polar, highly water soluble amphoteric compound. Imidazole is often used to elute tagged proteins bound to metal ions attached to the surface of beads in a chromatography column by displacing the His-tag from the metal ion and freeing the His-tagged proteins.

Product Specifications

Catalog ID I-902
CAS # 288-32-4
MW 68.08 g/mol
Storage/Handling Store at room temperature.
UN Number 3262
Item Class 8
Group Number III
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I-902-25 25 g $ 68.00
I-902-50 50 g $ 115.00
I-902-100 100 g $ 184.00
I-902-500 500 g $ 562.00

5X Phosphate Buffer, pH 8

Catalog ID Size Pricing
P-500-50 50 mL $ 58.00
P-500-100 100 mL $ 81.00
P-500-250 250 mL $ 115.00

Description

No description available

Product Specifications

Catalog ID P-500
Storage/Handling Store at 4°C.
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P-500-50 50 mL $ 58.00
P-500-100 100 mL $ 81.00
P-500-250 250 mL $ 115.00

Imidazole - 3M Recrystallized Solution, pH 6.0

Catalog ID Size Pricing
I-901-25 25 mL $ 68.00
I-901-50 50 mL $ 101.00
I-901-100 100 mL $ 156.00
I-901-250 250 mL $ 361.00

Description

No description available

Product Specifications

Catalog ID I-901
CAS # 288-32-4
MW 68.08 g/mol
Storage/Handling Store at 4°C.
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I-901-25 25 mL $ 68.00
I-901-50 50 mL $ 101.00
I-901-100 100 mL $ 156.00
I-901-250 250 mL $ 361.00

5X Phosphate Buffer + 3M Imidazole Solution

Catalog ID Size Pricing
I-905-100 100 mL $ 178.00

Description

No description available

Product Specifications

Catalog ID I-905
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I-905-100 100 mL $ 178.00

His-Tag Buffer Set

Catalog ID Size Pricing
I-906-250 1 kit $ 114.00

Description

No description available

Product Specifications

Catalog ID I-906
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I-906-250 1 kit $ 114.00

Mammalian Cell Lysis Buffer

Catalog ID Size Pricing
GB-180-100 100 mL $ 58.00
GB-180-500 500 mL $ 117.00

Description

Product Specifications

Catalog ID GB-180
Storage/Handling Store at 4°C.
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GB-180-100 100 mL $ 58.00
GB-180-500 500 mL $ 117.00

Tissue Cell Lysis Buffer

Catalog ID Size Pricing
GB-181-100 100 mL $ 58.00
GB-181-500 500 mL $ 113.00

Description

No description available

Product Specifications

Catalog ID GB-181
Storage/Handling Store at 4°C.
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GB-181-100 100 mL $ 58.00
GB-181-500 500 mL $ 113.00

Bacterial Cell Lysis Buffer

Catalog ID Size Pricing
GB-177-100 100 mL $ 58.00
GB-177-500 500 mL $ 113.00
GB-178 100 Preps $ 171.00

Description

No description available

Product Specifications

Catalog ID GB-177
Storage/Handling Store at 4°C. Do NOT freeze.
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GB-177-100 100 mL $ 58.00
GB-177-500 500 mL $ 113.00
GB-178 100 Preps $ 171.00

Yeast Lysis Buffer Kit plus Zymolyase

Catalog ID Size Pricing

Description

No description available

Product Specifications

Catalog ID GB-178
Storage/Handling Store at -20°C.
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Yeast Spheroplast Lysis Buffer

Catalog ID Size Pricing
GB-179-100 100 mL $ 58.00
GB-179-500 500 mL $ 113.00

Description

No description available

Product Specifications

Catalog ID GB-179
Storage/Handling Store at -20°C.
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GB-179-100 100 mL $ 58.00
GB-179-500 500 mL $ 113.00

HEPES, Free Acid

Catalog ID Size Pricing
H-400-100 100 g $ 34.00
H-400-250 250 g $ 68.00
H-400-500 500 g $ 103.00
H-400-1 1 kg $ 161.00
H-400-2 2 x 1 kg $ 268.00
H-400-5 5 kg $ 568.00

Description

HEPES is a buffering agent used in biochemistry and molecular biology that was selected and described by Good et al. It is a zwitterionic, piperazinic buffer that is useful for a pH range of 6.8 – 8.2. HEPES lacks the ability to form a significant complex with most metal ions and is recommended for use as a non-coordinating buffer in solutions with metal ions.

Product Specifications

Catalog ID H-400
CAS # 7365-45-9
MW 238.30 g/mol
Grade ULTRA PURE GRADE
Storage/Handling Store at room temperature.
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H-400-100 100 g $ 34.00
H-400-250 250 g $ 68.00
H-400-500 500 g $ 103.00
H-400-1 1 kg $ 161.00
H-400-2 2 x 1 kg $ 268.00
H-400-5 5 kg $ 568.00

Tris (Tris Base)

Catalog ID Size Pricing
T-400-500 500 g $ 34.00
T-400-1 1 kg $ 58.00
T-400-5 5 kg $ 218.00
T-400-10 10 kg $ 426.00

Description

GoldBio Tris has the highest purity available on the market, at more than 99.9% pure.  Short for Tris (hydroxymethyl) aminomethane (THAM), Tris is an organic compound often used in buffer solutions such as TAE or TBE for electrophoresis gels. Tris is highly soluble in water and is useful in the pH range 7.0-9.0 and is also used in the preparation of Laemmli buffer, one of the most common SDS-PAGE buffers.  In addition, it can also be used for many custom running and loading buffers, most often with glycine and SDS.

Product Specifications

Catalog ID T-400
CAS # 77-86-1
MW 121.14 g/mol
Grade ULTRA PURE GRADE
Storage/Handling Store desiccated at room temperature.
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T-400-500 500 g $ 34.00
T-400-1 1 kg $ 58.00
T-400-5 5 kg $ 218.00
T-400-10 10 kg $ 426.00

ACES

Catalog ID Size Pricing
A-010-25 25 g $ 27.00
A-010-50 50 g $ 42.00
A-010-100 100 g $ 53.00

Description

ACES is a zwitterionic buffering agent used in biochemistry and molecular biology that was first selected and described by Good et al. It is an acetamido buffer that is useful for a pH range of 6.1 – 7.5. ACES is used as buffering component in cell culture media, in both agarose and polyacrylamide electrophoresis, and in isoelectric focusing of proteins. ACES forms a complex with most common metals and formation constants should be taken into account when using this buffer in a solution containing metal ions. ACES absorbs UV light at 230 nm.

Product Specifications

Catalog ID A-010
CAS # 7365-82-4
MW 182.20 g/mol
Grade HIGH PURITY GRADE
Storage/Handling Store at room temperature.
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A-010-25 25 g $ 27.00
A-010-50 50 g $ 42.00
A-010-100 100 g $ 53.00

Bis-Tris

Catalog ID Size Pricing
B-020-100 100 g $ 55.00
B-020-250 250 g $ 83.00
B-020-500 500 g $ 127.00
B-020-1 1 kg $ 218.00

Description

Bis-Tris, also known as bis-Tris methane, is a zwitterionic buffering agent used in biochemistry and molecular biology. It is a bis(2-hydroxyethyl)amine and Tris family buffer that is useful for a pH range of 5.8 – 7.2. Bis Tris is a common component of many buffer systems for various types of electrophoresis. Bis-Tris may form a complex with some common metals, such as Cu(II) and Pb(II), and formation constants should be taken into account when using this buffer in a solution containing metal ions. Bis-Tris can be used as a substitute for the highly toxic buffer cacodylate.

Product Specifications

Catalog ID B-020
CAS # 6976-37-0
MW 209.24 g/mol
Storage/Handling Store at room temperature.
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B-020-100 100 g $ 55.00
B-020-250 250 g $ 83.00
B-020-500 500 g $ 127.00
B-020-1 1 kg $ 218.00

Boric Acid

Catalog ID Size Pricing
B-030-500 500 g $ 16.00
B-030-1 1 kg $ 27.00
B-030-5 5 kg $ 117.00

Description

Boric acid is commonly used for the preparation of buffers such as Tris-Borate-EDTA (TBE). Borate buffers have been used in capillary and gel electrophoresis for separating proteins and nucleic acids. The useful buffering range of boric acid is pH 8.2-10.1. In a basic solution, the salt of boric acid forms complex equilibria, with the tetraborate ion (B4O72-) being the most predominant species present. Boric acid is used in catalytic amounts to promote amidation between carboxylic acids and amines. It is capable of forming a complex with various carbohydrates, as well as with pyridine nucleotides, such as NAD+.

Product Specifications

Catalog ID B-030
CAS # 10043-35-3
MW 61.83 g/mol
Grade BIOTECHNOLOGY GRADE
Storage/Handling Store desiccated at room temperature.
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B-030-500 500 g $ 16.00
B-030-1 1 kg $ 27.00
B-030-5 5 kg $ 117.00

CAPS

Catalog ID Size Pricing
C-040-25 25 g $ 21.00
C-040-50 50 g $ 31.00
C-040-100 100 g $ 43.00
C-040-500 500 g $ 105.00

Description

CAPS is a zwitterionic buffering agent used in biochemistry and molecular biology. It is a cyclohexylamine buffer that is useful for a pH range of 9.7 – 11.1 and commonly used to study enzymatic processes above physiological pH. CAPS is an excellent buffer for the transfer of high molecular weight proteins to nitrocellulose membranes after SDS-PAGE. It is also used in protein sequencing. CAPS displays only weak or negligible complexation capabilities for some metals making it a suitable non-coordinating buffer for use in solutions containing metal ions. CAPS is suitable for use with the bicinchoninic acid (BCA) assay.

Product Specifications

Catalog ID C-040
CAS # 1135-40-6
MW 221.32 g/mol
Storage/Handling Store desiccated at room temperature.
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C-040-25 25 g $ 21.00
C-040-50 50 g $ 31.00
C-040-100 100 g $ 43.00
C-040-500 500 g $ 105.00

HEPES, Sodium Salt

Catalog ID Size Pricing
H-401-50 50 g $ 33.00
H-401-100 100 g $ 56.00
H-401-500 500 g $ 164.00
H-401-1 1 kg $ 274.00
H-401-2.5 2.5 kg $ 606.00
H-401-5 5 kg $ 1,053.00

Description

No description available

Product Specifications

Catalog ID H-401
CAS # 75277-39-3
MW 260.29 g/mol
Grade HIGH PURITY GRADE
Storage/Handling Store desiccated at room temperature.
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H-401-50 50 g $ 33.00
H-401-100 100 g $ 56.00
H-401-500 500 g $ 164.00
H-401-1 1 kg $ 274.00
H-401-2.5 2.5 kg $ 606.00
H-401-5 5 kg $ 1,053.00

MES Free Acid Monohydrate, Ultra Pure

Catalog ID Size Pricing
M-090-100 100 g $ 55.00
M-090-250 250 g $ 83.00
M-090-500 500 g $ 121.00
M-090-1 1 kg $ 207.00

Description

MES monohydrate is a buffering agent used in biochemistry and molecular biology that was selected and described by Good et al. It is a zwitterionic, morpholinic buffer that is useful for a pH range of 5.5 – 6.7 and commonly used for cell culture media, as a running buffer in electrophoresis, and for protein purification in chromatography. MES lacks the ability to form a complex with most metal ions and is recommended for use as a non-coordinating buffer in solutions with metal ions.

Product Specifications

Catalog ID M-090
CAS # 145224-94-8
MW 213.25 g/mol
Storage/Handling Store desiccated at room temperature.
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M-090-100 100 g $ 55.00
M-090-250 250 g $ 83.00
M-090-500 500 g $ 121.00
M-090-1 1 kg $ 207.00

MOPS Free Acid, Ultra Pure

Catalog ID Size Pricing
M-790-100 100 g $ 32.00
M-790-250 250 g $ 55.00
M-790-500 500 g $ 87.00
M-790-1 1 kg $ 141.00
M-790-2 2 x 1 kg $ 253.00
M-790-5 5 kg $ 578.00

Description

MOPS is a buffering agent used in biochemistry and molecular biology that was selected and described by Good et al. It is a zwitterionic, morpholinic buffer that is useful for a pH range of 6.5 – 7.9 and commonly used for cell culture media, as a running buffer in electrophoresis, and for protein purification in chromatography. MOPS lacks the ability to form a complex with most metal ions and is recommended for use as a non-coordinating buffer in solutions with metal ions.

Product Specifications

Catalog ID M-790
CAS # 1132-61-2
MW 209.26 g/mol
Storage/Handling Store desiccated at room temperature.
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M-790-100 100 g $ 32.00
M-790-250 250 g $ 55.00
M-790-500 500 g $ 87.00
M-790-1 1 kg $ 141.00
M-790-2 2 x 1 kg $ 253.00
M-790-5 5 kg $ 578.00

MOPS, Sodium Salt

Catalog ID Size Pricing
M-791-25 25 g $ 27.00
M-791-50 50 g $ 42.00
M-791-100 100 g $ 53.00
M-791-250 250 g $ 84.00
M-791-500 500 g $ 117.00
M-791-1 1 kg $ 186.00

Description

No description available

Product Specifications

Catalog ID M-791
CAS # 71119-22-7
MW 231.25 g/mol
Grade HIGH PURITY GRADE
Storage/Handling Store desiccated at room temperature.
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M-791-25 25 g $ 27.00
M-791-50 50 g $ 42.00
M-791-100 100 g $ 53.00
M-791-250 250 g $ 84.00
M-791-500 500 g $ 117.00
M-791-1 1 kg $ 186.00

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