Protein Expression & Purification

Proteins are fundamental macromolecules found in every biological system and form the basis of many fields of work from research into drug creation to industrial applications like fermentation. GoldBio protein products are ideal for every step of your protein synthesis. Our reagents and tools are of the highest quality available at a fraction of the prices of other vendors, so you can be assured that your research yields confident results without compromising your budget.
Showing 1-20 of 123 items.

1 kb DNA Ladder

1 kb DNA Ladder
Catalog ID Size Pricing
D010-500 500 µL $ 46.00
D010-1000 2x500 µL $ 86.00
D010-1500 3x500 µL $ 121.00
D010-2000 4x500 µL $ 161.00
D010-2500 5x500 µL $ 184.00
D010-3000 6x500 µL $ 201.00

Description

A unique combination of a number of proprietary plasmids digested with appropriate restriction enzymes and PCR products to yield 13 fragments, suitable for use as molecular weight standards for agarose gel electrophoresis. The DNA includes fragments ranging from 250-10,000 base pairs. The 1K and 3K bands have increased intensity to serve as reference points. The approximate mass of DNA in each band is provided (0.5 μg a load) for approximating the mass of DNA in comparably intense samples. The ladder is ready-to-use, and is premixed with loading buffer dye for direct loading on gel.

Product Specifications

Catalog ID D010
Storage/Handling Store at room temperature for 6 months. Store at -20°C for 24 months.
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D010-500 500 µL $ 46.00
D010-1000 2x500 µL $ 86.00
D010-1500 3x500 µL $ 121.00
D010-2000 4x500 µL $ 161.00
D010-2500 5x500 µL $ 184.00
D010-3000 6x500 µL $ 201.00

1 kb PLUS™ DNA Ladder

Catalog ID Size Pricing
D011-500 500 µL $ 58.00
D011-1000 2x500 µL $ 109.00
D011-1500 3x500 µL $ 155.00
D011-2000 4x500 µL $ 196.00
D011-2500 5x500 µL $ 230.00
D011-3000 6x500 µL $ 253.00

Description

The 1 kb PLUS™ is composed of 14 individual DNA fragments: 25K, 10K, 8K, 6K, 5K, 4K, 3K, 2.5K, 2K, 1500, 1000, 750, 500, 250 base pairs. This product contains two enhanced bands (3K and 1K bp) for easy reference. The ladder is ready-to-use, and is premixed with loading buffer dye for direct loading on gel.

Product Specifications

Catalog ID D011
Storage/Handling Store at room temperature for 6 months. Store at -20°C for 24 months.
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D011-500 500 µL $ 58.00
D011-1000 2x500 µL $ 109.00
D011-1500 3x500 µL $ 155.00
D011-2000 4x500 µL $ 196.00
D011-2500 5x500 µL $ 230.00
D011-3000 6x500 µL $ 253.00

1,2-Dipalmitoyl-rac-glycero-3-phosphocholine

Catalog ID Size Pricing
D-226-500 500 mg $ 155.00
D-226-1 1 g $ 290.00
D-226-2.5 2.5 g $ 677.00
D-226-5 5 g $ 1,265.00

Description

No description available

Product Specifications

Catalog ID D-226
CAS # 2644-64-6
MW 734.04 g/mol
Storage/Handling Store at -20°C. Protect from light.
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D-226-500 500 mg $ 155.00
D-226-1 1 g $ 290.00
D-226-2.5 2.5 g $ 677.00
D-226-5 5 g $ 1,265.00

100 bp DNA Ladder

Catalog ID Size Pricing
D001-500 500 µL $ 46.00
D001-1000 2x500 µL $ 86.00
D001-1500 3x500 µL $ 121.00
D001-2000 4x500 µL $ 161.00
D001-2500 5x500 µL $ 184.00
D001-3000 6x500 µL $ 201.00

Description

A unique combination of PCR products and a number of proprietary plasmids digested with appropriate restriction enzymes to yield 11 fragments, suitable for use as molecular weight standards for agarose gel electrophoresis. The DNA includes fragments ranging from 100-1,500 base pairs. The 500 and 1,500 base pair bands have increased intensity to serve as reference points. The approximate mass of DNA in each band is provided (0.5 μg a load) for approximating the mass of DNA in comparably intense samples of similar size. The ladder is ready-to-use, and is premixed with loading buffer dye for direct loading on gel.

Product Specifications

Catalog ID D001
Storage/Handling Store at room temperature for 6 months. Store at -20°C for 24 months.
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D001-500 500 µL $ 46.00
D001-1000 2x500 µL $ 86.00
D001-1500 3x500 µL $ 121.00
D001-2000 4x500 µL $ 161.00
D001-2500 5x500 µL $ 184.00
D001-3000 6x500 µL $ 201.00

100 bp PLUS™ DNA Ladder

Catalog ID Size Pricing
D003-500 500 µL $ 46.00
D003-1000 2x500 µL $ 86.00
D003-1500 3x500 µL $ 127.00
D003-2000 4x500 µL $ 161.00
D003-2500 5x500 µL $ 184.00
D003-3000 6x500 µL $ 201.00

Description

A unique combination of PCR products and a number of proprietary plasmids digested with appropriate restriction enzymes to yield 12 fragments, suitable for use as molecular weight standards for agarose gel electrophoresis. The DNA includes fragments ranging from 100-3,000 base pairs. The 500 and 1,500 base pair bands have increased intensity to serve as reference points. The approximate mass of DNA in each band is provided (0.5 μg a load) for approximating the mass of DNA in comparably intense samples of similar size.The ladder is ready-to-use, and is premixed with loading buffer dye for direct loading on gel.

Product Specifications

Catalog ID D003
Storage/Handling Store at room temperature for 6 months. Store at -20°C for 24 months.
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D003-500 500 µL $ 46.00
D003-1000 2x500 µL $ 86.00
D003-1500 3x500 µL $ 127.00
D003-2000 4x500 µL $ 161.00
D003-2500 5x500 µL $ 184.00
D003-3000 6x500 µL $ 201.00

4-Nitrophenyl phosphate, disodium salt hexahydrate

Catalog ID Size Pricing
N-380-50 50 g $ 74.00
N-380-100 100 g $ 126.00
N-380-250 250 g $ 279.00
N-380-500 500 g $ 475.00

Description

No description available

Product Specifications

Catalog ID N-380
CAS # 333338-18-4
MW 371.15 g/mol
Grade MOLECULAR BIOLOGY GRADE
Storage/Handling Store at -20°C. Protect from light.
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N-380-50 50 g $ 74.00
N-380-100 100 g $ 126.00
N-380-250 250 g $ 279.00
N-380-500 500 g $ 475.00

5-Fluoroorotic acid monohydrate (5-FOA)

Catalog ID Size Pricing
F-230-2.5 2.5 g $ 58.00
F-230-5 5 g $ 110.00
F-230-10 10 g $ 184.00
F-230-25 25 g $ 338.00

Description

5-Fluoroorotic Acid (5-FOA) is widely used in the field of yeast molecular genetics. The toxicity of 5-FOA to yeast cells with a functioning URA3 gene which encodes orotidine-5′-P decarboxylase (OMP decarboxylase), makes it especially useful for the selection and identification of mutant yeast strains. 5-FOA is most often applied with Saccharomyces cerevisiae (URA3), although it has been used in other strains including Schizosaccharomyces pombe (URA4 and URA5), Candida albicans (URA3) and the bacterium E. coli (pyrF). The use of 5-FOA in the yeast 2-hybrid system allows for the construction of activation domain hybrid libraries to identify protein-protein interactions.

Product Specifications

Catalog ID F-230
CAS # 220141-70-8
MW 192.10 g/mol
Grade MOLECULAR BIOLOGY GRADE
Storage/Handling Store at -20°C. Protect from light.
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F-230-2.5 2.5 g $ 58.00
F-230-5 5 g $ 110.00
F-230-10 10 g $ 184.00
F-230-25 25 g $ 338.00

50 bp DNA Ladder

50 bp DNA Ladder
Catalog ID Size Pricing
D100-500 500 µL $ 58.00
D100-1000 2x500 µL $ 109.00
D100-1500 3x500 µL $ 155.00
D100-2000 4x500 µL $ 196.00
D100-2500 5x500 µL $ 230.00
D100-3000 6x500 µL $ 253.00

Description

A unique combination of PCR products and a number of proprietary plasmids digested with appropriate restriction enzymes to yield 17 fragments, suitable for gel electrophoresis. The DNA includes fragments ranging from 50-1,500 base pairs. The 200 and 500 base pair bands have increased intensity to serve as reference points. The approximate mass of DNA in each band is provided (0.5 g a load) for approximating the mass of DNA in comparably intense samples of a similar size.The ladder is ready-to-use, and is premixed with loading buffer dye for direct loading on gel.

Product Specifications

Catalog ID D100
Storage/Handling Store at room temperature for 6 months. Store at -20°C for 24 months.
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D100-500 500 µL $ 58.00
D100-1000 2x500 µL $ 109.00
D100-1500 3x500 µL $ 155.00
D100-2000 4x500 µL $ 196.00
D100-2500 5x500 µL $ 230.00
D100-3000 6x500 µL $ 253.00

AEBSF

Catalog ID Size Pricing
A-540-500 500 mg $ 50.00
A-540-1 1 g $ 91.00
A-540-2.5 2.5 g $ 200.00
A-540-5 5 g $ 299.00
A-540-10 10 g $ 541.00
A-540-25 25 g $ 1,219.00

Description

AEBSF is an irreversible serine protease inhibitor. AEBSF has been shown to inhibit trypsin, chymotrypsin, plasmin, kallikrein and thrombin. It inhibits by acylation of the active site of the enzyme. As an alternative to PMSF and DFP, AEBSF offers lower toxicity, improved solubility in water and improved stability in aqueous solutions. AEBSF has been used in cell culture in concentrations of up to 0.25 mM. Aqueous solutions are stable between pH 5-6; limited stability above pH 7.5.

Product Specifications

Catalog ID A-540
CAS # 30827-99-7
MW 239.69 g/mol
Grade MOLECULAR BIOLOGY GRADE
Storage/Handling Store at -20°C. Protect from light.
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A-540-500 500 mg $ 50.00
A-540-1 1 g $ 91.00
A-540-2.5 2.5 g $ 200.00
A-540-5 5 g $ 299.00
A-540-10 10 g $ 541.00
A-540-25 25 g $ 1,219.00

Agarose LE (Molecular Biology Grade)

Catalog ID Size Pricing
A-201-100 100 g $ 92.00   $ 79.00
A-201-500 500 g $ 338.00   $ 288.00
A-201-1000 1 kg $ 562.00   $ 478.00

Description

Agarose LE (Low Electroendosmosis) is Molecular Biology Grade and is specifically designed for the superior separation of nucleic acids, with maximally crisp band resolution, and is also ideal for cloning and cloning related experiments.

GoldBio Agarose LE is refined in an advanced process that excludes the use of organic solvents. The result is a cleaner end-product with significantly reduced environmental impact. The agarose can be used for analyses of nucleic acids from 50 bp to 25 kbp, protein electrophoresis and various blotting protocols.

The low EEO of the agarose promotes increased electrophoretic mobility, yielding improved resolution and shorter run times. This also allows macromolecules and larger particles (subcellular fragments, viruses, etc.) to migrate more freely through the gel matrix. The consistently low EEO reduces band distortion (caused by counterflow) that can result from the presence of excessive sulfate-rich negative ions.

Agarose is a natural product that forms an inert matrix used in electrophoresis, chromatography and other molecular biology and biochemistry techniques. Likewise, it is neutral and easily derivatizable, so it is easy to bind to its structure proteins like enzymes, antigens or antibodies. Toxicity absence makes working with agarose very convenient.

Product Specifications

Catalog ID A-201
CAS # 9012-36-6
Storage/Handling Store at room temperature.
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A-201-100 100 g $ 92.00
$ 79.00
A-201-500 500 g $ 338.00
$ 288.00
A-201-1000 1 kg $ 562.00
$ 478.00

High Resolution Agarose (For Nucleotides < 1kb)

Catalog ID Size Pricing
A-202-100 100 g $ 254.00
A-202-500 500 g $ 1,207.00

Description

No description available

Product Specifications

Catalog ID A-202
CAS # 9012-36-6
Storage/Handling Store at room temperature.
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A-202-100 100 g $ 254.00
A-202-500 500 g $ 1,207.00

Ampicillin (Sodium), USP Grade

Catalog ID Size Pricing
A-301-5 5 g $ 23.00
A-301-10 10 g $ 34.00
A-301-25 25 g $ 46.00
A-301-100 100 g $ 172.00
A-301-500 500 g $ 822.00

Description

Ampicillin sodium is a member of the extended-spectrum β-lactam family and similar in structure to penicillin. It is used in the selection of antibiotic cell hybrids and has a high rate of action against many gram-negative bacterial strains. Ampicillin sodium targets non-ESBL (Extended Spectrum β-lactamase) bacteria including Staphylococcus and Streptococcus species and medically important enteric pathogens such as Shigella and Salmonella. It has been found to be effective against certain β-lactam sensitive VRE or vancomycin resistant Enterococcus species. Resistance to ampicillin is routinely utilized as a selectable marker to confirm successful cell transformation. Ampicillin Sodium is freely soluble in water.

Product Specifications

Catalog ID A-301
CAS # 69-52-3
MW 371.39 g/mol
Storage/Handling Store desiccated at -20°C.
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A-301-5 5 g $ 23.00
A-301-10 10 g $ 34.00
A-301-25 25 g $ 46.00
A-301-100 100 g $ 172.00
A-301-500 500 g $ 822.00

BCIP

Catalog ID Size Pricing
B-500-1 1 g $ 63.00
B-500-10 10 g $ 272.00
B-500-25 25 g $ 675.00
B-500-50 50 g $ 1,281.00

Description

BCIP is a white to off-white powder that is used as a substrate for visualization of alkaline phosphatase. The end product is insoluble and purple when used with nitroblue tetrazolium. This final product can be visually detected. BCIP is DMF soluble.

Product Specifications

Catalog ID B-500
CAS # 6578-06-9
MW 433.63 g/mol
Grade MOLECULAR BIOLOGY GRADE
Storage/Handling Store at -20°C. Protect from light.
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B-500-1 1 g $ 63.00
B-500-10 10 g $ 272.00
B-500-25 25 g $ 675.00
B-500-50 50 g $ 1,281.00

Benzamidine hydrochloride monohydrate

Catalog ID Size Pricing
B-050-100 100 g $ 63.00
B-050-500 500 g $ 225.00
B-050-1 1 kg $ 375.00
B-050-2.5 2.5 kg $ 773.00

Description

Benzamidine hydrochloride monohydrate is an aromatic amidine. It is used in protein extractions as a strong reversible inhibitor of serine proteases such as trypsin, thrombin, plasmin and other trypsin-like proteases. Compared to aprotinin, benzamidine is considered to be just as effective in preventing the degradation of glucogon.

Product Specifications

Catalog ID B-050
CAS # 1670-14-0
MW 174.63 g/mol
Grade MOLECULAR BIOLOGY GRADE
Storage/Handling Store at -20°C. Protect from light.
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B-050-100 100 g $ 63.00
B-050-500 500 g $ 225.00
B-050-1 1 kg $ 375.00
B-050-2.5 2.5 kg $ 773.00

BLUEstain™ 2 Protein ladder, 5-245 kDa

Catalog ID Size Pricing
P008-500 500 µL $ 115.00
P008-1000 2x500 µL $ 224.00
P008-1500 3x500 µL $ 321.00
P008-2000 4x500 µL $ 417.00
P008-2500 5x500 µL $ 506.00
P008-3000 6x500 µL $ 591.00

Description

The BLUEstain™ 2 Protein ladder is a three-color protein standard with 13 prestained proteins, covering a wide range molecular weights from 3.5 to 245 kDa. Proteins are covalently coupled with a blue chromophore except for two reference bands (one green and one red band at 25 kDa and 75 kDa respectively) when separated on SDS-PAGE (Tris-glycine buffer). 


The BLUEstain™ 2 Protein ladder is designed for monitoring protein separation during SDS-polyacrylamide gel electrophoresis, verification of Western transfer efficiency on membranes (PVDF, nylon, or nitrocellulose) and for approximating the size of proteins. The ladder is supplied in gel loading buffer and is ready to use. Do not heat, dilute or add reducing agent before loading.

Product Specifications

Catalog ID P008
Storage/Handling Store at 4°C for 3 months. Store at -20°C for 24 months.
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P008-500 500 µL $ 115.00
P008-1000 2x500 µL $ 224.00
P008-1500 3x500 µL $ 321.00
P008-2000 4x500 µL $ 417.00
P008-2500 5x500 µL $ 506.00
P008-3000 6x500 µL $ 591.00

BLUEstain™ Protein ladder, 11-245 kDa

Catalog ID Size Pricing
P007-500 500 µL $ 115.00
P007-1000 2x500 µL $ 224.00
P007-1500 3x500 µL $ 321.00
P007-2000 4x500 µL $ 417.00
P007-2500 5x500 µL $ 506.00
P007-3000 6x500 µL $ 591.00

Description

The BLUEstain™ Protein ladder is a three-color protein standard with 12 prestained proteins covering a wide range molecular weights from ~11 to 245 kDa. Proteins are covalently coupled with a bluechromophore except for two reference bands (one green and one red band at 25 kDa and 75 kDa respectively) when separated on SDS-PAGE (Tris-glycine buffer). The BLUEstain™ Protein ladder, is designed for monitoring protein separation during SDS-polyacrylamide gel electrophoresis, verification of Western transfer efficiency on membranes (PVDF, nylon, or nitrocellulose) and for approximating the size of proteins. The ladder is supplied in gel loading buffer and is ready to use.

Product Specifications

Catalog ID P007
Storage/Handling Store at 4°C for 3 months. Store at -20°C for 24 months.
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P007-500 500 µL $ 115.00
P007-1000 2x500 µL $ 224.00
P007-1500 3x500 µL $ 321.00
P007-2000 4x500 µL $ 417.00
P007-2500 5x500 µL $ 506.00
P007-3000 6x500 µL $ 591.00

Carbenicillin (Disodium)

Catalog ID Size Pricing
C-103-5 5 g $ 46.00
C-103-25 25 g $ 150.00
C-103-50 50 g $ 264.00
C-103-100 100 g $ 450.00
C-103-250 250 g $ 911.00

Description

Carbenicillin is a member of the penicillin β-lactam antibiotics. However, unlike most β-lactams, carbenicillin disodium is limited to primarily gram-negative bacteria including Pseudomonas aeruginosa and common enteric species. It can be used in selection protocols and is often preferred over ampicillin due to its increased stability. This allows for less contamination of unwanted bacterial colonies. In addition, carbenicillin has been used to regulate bacterial growth in plants, yielding low regeneration frequencies. It can be used in genetic transformation applications to select for AmpR transformed cells.

Product Specifications

Catalog ID C-103
CAS # 4800-94-6
MW 422.36 g/mol
Grade MOLECULAR BIOLOGY GRADE
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C-103-5 5 g $ 46.00
C-103-25 25 g $ 150.00
C-103-50 50 g $ 264.00
C-103-100 100 g $ 450.00
C-103-250 250 g $ 911.00

Cefotaxime (Sodium), USP Grade

Catalog ID Size Pricing
C-104-1 1 g $ 23.00
C-104-5 5 g $ 41.00
C-104-25 25 g $ 172.00

Description

Cefotaxime is a third generation, broad-spectrum cephalosporin antibiotic effective against various gram-negative and gram-positive bacteria. However, unlike many other cephalosporins, cefotaxime is not effective against Pseudomonas aeruginosa bacteria. Cefotaxime is often used in Agrobacterium tumefaciens-mediated transformations to regulate bacterial growth. It has been shown to exhibit a synergistic effect with Vancomycin in certain conditions.

Product Specifications

Catalog ID C-104
CAS # 64485-93-4
MW 477.45 g/mol
Storage/Handling Store desiccated at -20°C.
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C-104-1 1 g $ 23.00
C-104-5 5 g $ 41.00
C-104-25 25 g $ 172.00

Cellulase R-10

There is no image for Cellulase R-10 Cellulase R-10
Catalog ID Size Pricing
C8001.0001 1 g $ 83.00
C8001.0005 5 g $ 162.00
C8001.0010 10 g $ 253.00

Description

No description available

Product Specifications

Catalog ID C8001
CAS # 9012-54-8
Storage/Handling Store at room temperature.
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C8001.0001 1 g $ 83.00
C8001.0005 5 g $ 162.00
C8001.0010 10 g $ 253.00

Chloramphenicol, USP Grade

Catalog ID Size Pricing
C-105-5 5 g $ 12.00
C-105-25 25 g $ 23.00
C-105-100 100 g $ 79.00

Description

Chloramphenicol, also known as chlornitromycin, is a broad-spectrum, bacteriostatic antibiotic derived from Streptomyces venezuelae. It is effective against several gram-positive and gram-negative bacteria and commonly used in researching protein synthesis and to select for chloramphenicol-resistant transformed cells or the bacterial CAT gene.

Product Specifications

Catalog ID C-105
CAS # 56-75-7
MW 323.13 g/mol
Grade MOLECULAR BIOLOGY GRADE
Storage/Handling Store desiccated at -20°C.
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C-105-5 5 g $ 12.00
C-105-25 25 g $ 23.00
C-105-100 100 g $ 79.00

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