NTA Resins

His-tag purification is a vital part of modern research. Impure extracts generally contain a wide range of proteins with diverse biological functions and chemistry that need to be separated. GoldBio's NTA cross-linked agarose resins consist of nitriloacetic acid groups ligated by stable ether linkages via a spacer arm. NTA is a tetradentate chelating agent, covalently coupled to cross-linked agarose beads. NTA resins generally give a higher specificity than IDA resins, work better in high EDTA concentrations and are preferred in denatured or reduced conditions.
Showing 1-3 of 3 items.

Nickel NTA Agarose Beads

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Catalog ID Size Pricing
H-350-5 5 mL $ 64.00
H-350-10 10 mL $ 103.00
H-350-25 25 mL $ 224.00
H-350-50 50 mL $ 387.00
H-350-100 100 mL $ 730.00

Description

Nickel-NTA Agarose Resin has a binding capacity of ~50 mg/ml. In addition, it allows for purification of proteins under native or denaturing conditions. GoldBio Nickel Agarose works very well with the His-Tag Buffer Set.

NTA cross-linked Agarose resin consists of nitrilotriacetic acid groups ligated by stable ether linkages via a spacer arm. NTA is a tetravalent chelating agent, covalently coupled to cross-linked agarose beads, providing a higher specificity and lower ion leaching than IDA linked resins. NTA resins have also been shown to be more robust in the presence of higher concentrations of EDTA, but may require a higher imidazole concentration for protein elution. This resin is loaded with Ni 2+. The resulting, ready-to-use resin is ideal for rapid purifications of His-tagged proteins.

Product Specifications

Catalog ID H-350
Storage/Handling store at 4°C. Do NOT freeze.
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H-350-5 5 mL $ 64.00
H-350-10 10 mL $ 103.00
H-350-25 25 mL $ 224.00
H-350-50 50 mL $ 387.00
H-350-100 100 mL $ 730.00

Nickel NTA HTC Agarose Beads

There is no image for Nickel NTA HTC Agarose Beads Nickel NTA HTC Agarose Beads
Catalog ID Size Pricing
H-355-25 25 mL $ 296.00
H-355-100 100 mL $ 993.00
H-355-500 500 mL $ 4,269.00

Description

Nickel NTA HTC Agarose Resin has a binding capacity of ~60 mg/ml. In addition, it allows for purification of proteins under native or denaturing conditions. GoldBio Nickel Agarose works very well with the His-Tag Buffer Set.

NTA cross-linked Agarose resin consists of nitrilotriacetic acid groups ligated by stable ether linkages via a spacer arm. NTA is a tetravalent chelating agent, covalently coupled to cross-linked agarose beads, providing a higher specificity and lower ion leaching than IDA linked resins. NTA resins have also been shown to be more robust in the presence of higher concentrations of EDTA, but may require a higher imidazole concentration for protein elution. This resin is loaded with Ni 2+. The resulting, ready-to-use resin is ideal for rapid purifications of His-tagged proteins.

Product Specifications

Catalog ID H-355
Storage/Handling store at 4°C. Do NOT freeze.
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Quick Add
H-355-25 25 mL $ 296.00
H-355-100 100 mL $ 993.00
H-355-500 500 mL $ 4,269.00

Nickel NTA Magnetic Agarose Beads

There is no image for Nickel NTA Magnetic Agarose Beads Nickel NTA Magnetic Agarose Beads
Catalog ID Size Pricing
H-351-2 2 mL $ 103.00
H-351-5 5 mL $ 232.00
H-351-10 10 mL $ 421.00

Description

Nickel NTA Magnetic Agarose Beads are a rapid and easy small scale purification of histidine-tagged proteins. This resin consists of magnetic agarose derivatized with Nitrilotriacetic (NTA) and provides good properties working in native or denaturing conditions. Magnetic agarose beads provide a convenient and quick method for purification without the need for pipetting or centrifugation. Washing, binding and elution steps will require a magnetic device.

NTA cross-linked resins consist of nitrilotriacetic acid groups ligated by stable ether linkages via a spacer arm. NTA is a tetravalent chelating agent which provides a higher specificity and lower ion leaching than IDA linked resins. NTA resins have also been shown to be more robust in the presence of higher concentrations of EDTA, but may require a higher imidazole concentration for protein elution. This resin is loaded with Ni 2+. The resulting, ready-to-use resin is ideal for rapid purifications of His-tagged proteins.

Product Specifications

Catalog ID H-351
Storage/Handling store at 4°C. Do NOT freeze.
View
Quick Add
H-351-2 2 mL $ 103.00
H-351-5 5 mL $ 232.00
H-351-10 10 mL $ 421.00

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