Affinity Reagents

Affinity reagents and affinity-based tools utilize complex networks of specific interactions between various molecules for use in the separation, purification or analysis of proteins. Affinity purification requires an affinity ligand to be coupled to a matrix, which allows capture of the intended product from a complex mixture. Gold Biotechnology’s affinity reagents are activated beads or preactivated resins for use in coupling of affinity ligands and immobilization. Our affinity beads are specifically engineered to meet your specialized needs, whether it be laboratory scale experiments or industry scale separations. GoldBio’s agarose beads are conveniently cross-linked to include any of the following: aminoethyl or glyoxal groups, glutathione S-transferase, Protein A, Protein G, streptavidin and His-tag metal ions such as nickel, cobalt, copper or zinc.

Showing 1-20 of 52 items.

Cobalt Agarose Beads (High Density)

Catalog ID Size Pricing
H-310-5 5 mL $ 45.00
H-310-10 10 mL $ 79.00
H-310-25 25 mL $ 179.00
H-310-50 50 mL $ 298.00
H-310-100 100 mL $ 546.00
H-310-500 500 mL $ 2,184.00
H-310-C8 Column: 8 x 1 mL $ 157.00
H-310-C5 Column: 5 x 5 mL $ 466.00

Description

Available in bulk or in prepacked columns, cobalt has a binding capacity of >25 mg/ml with approximately 60 kDa protein. Cobalt binds fewer host protein contaminants, resulting in lower background than nickel resins and no metal contamination in eluted histidine-tagged protein sample. In addition, it allows for purification of proteins under native or denaturing conditions. Cobalt Agarose works very well with Ni/Cu/Co Resin Wash Buffer and Histidine Protein Elution Buffer.

Product Specifications

Catalog ID H-310
Storage/Handling store at 4°C. Do NOT freeze.
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H-310-5 5 mL $ 45.00
H-310-10 10 mL $ 79.00
H-310-25 25 mL $ 179.00
H-310-50 50 mL $ 298.00
H-310-100 100 mL $ 546.00
H-310-500 500 mL $ 2,184.00
H-310-C8 Column: 8 x 1 mL $ 157.00
H-310-C5 Column: 5 x 5 mL $ 466.00

Nickel Agarose Beads (High Density)

Catalog ID Size Pricing
H-320-5 5 mL $ 45.00
H-320-10 10 mL $ 79.00
H-320-25 25 mL $ 179.00
H-320-50 50 mL $ 298.00
H-320-100 100 mL $ 546.00
H-320-500 500 mL $ 2,184.00
H-320-C8 Column: 8x1 mL $ 157.00
H-320-C5 Column: 5x5 mL $ 466.00

Description

Nickel Agarose Resin is available in bulk or in prepacked columns. It has a binding capacity of >25 mg/ml with approximately 60 kDa protein. In addition, it allows for purification of proteins under native or denaturing conditions. GoldBio Nickel Agarose works very well with the His-Tag Buffer Set.

IDA cross-linked Agarose resin consists of iminodiacetic acid groups ligated by stable ether linkages via a spacer arm. IDA is a tridentate chelating agent, covalently coupled to cross-linked agarose beads. This resin is loaded with Ni2+. The resulting, ready-to-use resin is ideal for rapid purifications of His-tagged proteins.

Affinity Chromatography is based on the interaction between certain superficial protein residues (histidines, cysteines and to a lesser extent tryptophans), with transition metal cations, forming chelates.

Product Specifications

Catalog ID H-320
Storage/Handling store at 4°C. Do NOT freeze.
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H-320-5 5 mL $ 45.00
H-320-10 10 mL $ 79.00
H-320-25 25 mL $ 179.00
H-320-50 50 mL $ 298.00
H-320-100 100 mL $ 546.00
H-320-500 500 mL $ 2,184.00
H-320-C8 Column: 8x1 mL $ 157.00
H-320-C5 Column: 5x5 mL $ 466.00

4% HTC Agarose Beads, Standard

Catalog ID Size Pricing
R-200-500 500 mL $ 739.00
R-200-1000 1 L $ 1,044.00

Description

No description available

Product Specifications

Catalog ID R-200
Storage/Handling store at 4°C. Do NOT freeze.
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R-200-500 500 mL $ 739.00
R-200-1000 1 L $ 1,044.00

6% HTC Agarose Beads, Standard

Catalog ID Size Pricing
R-201-500 500 mL $ 801.00
R-201-1000 1 L $ 1,089.00

Description

No description available

Product Specifications

Catalog ID R-201
Storage/Handling store at 4°C. Do NOT freeze.
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R-201-500 500 mL $ 801.00
R-201-1000 1 L $ 1,089.00

Aminoethyl (Low Density) 6% Agarose Beads, Crosslinked

Catalog ID Size Pricing
A-501-25 25 mL $ 146.00
A-501-100 100 mL $ 483.00

Description

No description available

Product Specifications

Catalog ID A-501
Storage/Handling store at 4°C. Do NOT freeze.
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A-501-25 25 mL $ 146.00
A-501-100 100 mL $ 483.00

Aminoethyl (Very Low Density) 4% Agarose Beads, Crosslinked

Catalog ID Size Pricing
A-500-25 25 mL $ 139.00
A-500-100 100 mL $ 476.00

Description

No description available

Product Specifications

Catalog ID A-500
Storage/Handling store at 4°C. Do NOT freeze.
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A-500-25 25 mL $ 139.00
A-500-100 100 mL $ 476.00

Cobalt HTC Agarose Beads

Catalog ID Size Pricing
R-203-5 5 mL $ 64.00
R-203-25 25 mL $ 272.00
R-203-100 100 mL $ 685.00
R-203-500 500 mL $ 2,788.00

Description

Cobalt HTC 6% Agarose Beads are suitable for downstream purification using IMAC. These resins combine the advantages of Cobalt purification with the high flow rates of the HTC resin (70% higher than commercial standards).

Product Specifications

Catalog ID R-203
Storage/Handling store at 4°C. Do NOT freeze.
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R-203-5 5 mL $ 64.00
R-203-25 25 mL $ 272.00
R-203-100 100 mL $ 685.00
R-203-500 500 mL $ 2,788.00

Copper Agarose Beads (Low Density)

Catalog ID Size Pricing
H-311-25 25 mL $ 218.00
H-311-100 100 mL $ 577.00

Description

No description available

Product Specifications

Catalog ID H-311
Storage/Handling store at 4°C. Do NOT freeze.
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H-311-25 25 mL $ 218.00
H-311-100 100 mL $ 577.00

Imidazole, Recrystallized

Catalog ID Size Pricing
I-902-25 25 g $ 68.00
I-902-50 50 g $ 115.00
I-902-100 100 g $ 184.00
I-902-500 500 g $ 562.00

Description

Imidazole is a planar 5-membered ring. It is a highly polar, highly water soluble amphoteric compound. Imidazole is often used to elute tagged proteins bound to metal ions attached to the surface of beads in a chromatography column by displacing the His-tag from the metal ion and freeing the His-tagged proteins.

Product Specifications

Catalog ID I-902
CAS # 288-32-4
MW 68.08 g/mol
Storage/Handling Store at room temperature.
UN Number 3262
Item Class 8
Group Number III
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I-902-25 25 g $ 68.00
I-902-50 50 g $ 115.00
I-902-100 100 g $ 184.00
I-902-500 500 g $ 562.00

Glutathione Agarose Resin

Catalog ID Size Pricing
G-250-5 5 mL $ 84.00
G-250-10 10 mL $ 158.00
G-250-50 50 mL $ 610.00
G-250-100 100 mL $ 953.00

Description

Glutathione has been covalently linked for use in affinity purification of glutathione-S-transferase (GST) and GST fusion proteins.

Product Specifications

Catalog ID G-250
Storage/Handling store at 4°C. Do NOT freeze.
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G-250-5 5 mL $ 84.00
G-250-10 10 mL $ 158.00
G-250-50 50 mL $ 610.00
G-250-100 100 mL $ 953.00

Glyoxal (High Density) 4% Agarose Beads, Crosslinked

Catalog ID Size Pricing
G-301-25 25 mL $ 160.00
G-301-100 100 mL $ 484.00

Description

No description available

Product Specifications

Catalog ID G-301
Storage/Handling store at 4°C. Do NOT freeze.
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G-301-25 25 mL $ 160.00
G-301-100 100 mL $ 484.00

Glyoxal (High Density) 6% Agarose Beads, Crosslinked

Catalog ID Size Pricing
G-303-25 25 mL $ 164.00
G-303-100 100 mL $ 491.00

Description

No description available

Product Specifications

Catalog ID G-303
Storage/Handling store at 4°C. Do NOT freeze.
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G-303-25 25 mL $ 164.00
G-303-100 100 mL $ 491.00

Glyoxal (Low Density) 4% Agarose Beads, Crosslinked

Catalog ID Size Pricing
G-300-25 25 mL $ 168.00
G-300-100 100 mL $ 380.00

Description

No description available

Product Specifications

Catalog ID G-300
Storage/Handling store at 4°C. Do NOT freeze.
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G-300-25 25 mL $ 168.00
G-300-100 100 mL $ 380.00

Glyoxal (Low Density) 6% Agarose Beads, Crosslinked

Catalog ID Size Pricing
G-302-25 25 mL $ 121.00
G-302-100 100 mL $ 385.00

Description

No description available

Product Specifications

Catalog ID G-302
Storage/Handling store at 4°C. Do NOT freeze.
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G-302-25 25 mL $ 121.00
G-302-100 100 mL $ 385.00

Metal Free Agarose Beads (High Density)

Catalog ID Size Pricing
H-300-25 25 mL $ 218.00
H-300-100 100 mL $ 577.00

Description

No description available

Product Specifications

Catalog ID H-300
Storage/Handling store at 4°C. Do NOT freeze.
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H-300-25 25 mL $ 218.00
H-300-100 100 mL $ 577.00

Metal Free Agarose Beads (Low Density)

Catalog ID Size Pricing
H-301-25 25 mL $ 218.00
H-301-100 100 mL $ 577.00

Description

No description available

Product Specifications

Catalog ID H-301
Storage/Handling store at 4°C. Do NOT freeze.
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H-301-25 25 mL $ 218.00
H-301-100 100 mL $ 577.00

Nickel Agarose Beads (Low Density)

Catalog ID Size Pricing
H-321-25 25 mL $ 218.00
H-321-100 100 mL $ 577.00

Description

Low Density Nickel Agarose Resin is available in bulk or in prepacked columns. It has a binding capacity of >12 mg/ml with approximately 60 kDa protein. It works very well with the His-Tag Buffer Set.

IDA cross-linked Agarose resin consists of iminodiacetic acid groups ligated by stable ether linkages via a spacer arm. IDA is a tridentate chelating agent, covalently coupled to cross-linked agarose beads. This resin is loaded with Ni2+. The resulting, ready-to-use resin is ideal for rapid purifications of His-tagged proteins.

Affinity Chromatography is based on the interaction between certain superficial protein residues (histidines, cysteines and to a lesser extent tryptophans), with transition metal cations, forming chelates.

Product Specifications

Catalog ID H-321
Storage/Handling store at 4°C. Do NOT freeze.
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H-321-25 25 mL $ 218.00
H-321-100 100 mL $ 577.00

Nickel HTC Agarose Beads

Catalog ID Size Pricing
R-202-5 5 mL $ 64.00
R-202-25 25 mL $ 272.00
R-202-100 100 mL $ 685.00
R-202-500 500 mL $ 2,788.00
R-202-1000 1000 mL $ 5,016.00

Description

Nickel HTC 6% Agarose Beads are suitable for downstream purification using IMAC. These resins combine the advantages of Nickel purification with the high flow rates of the HTC resin (70% higher than commercial standards).

HTC beads are high throughput beads designed to meet the demand for industrial process separation. Their rigidity and mechanical resistance permits high flow rates, with good resolution in a minimum time frame, making these beads ideal for process-scale use. HTC beads are highly crosslinked 4% and 6% agarose matrices and have excellent physical and chromatographic qualities. They are autoclavable. These beads are an ideal support for the immobilization of ligands for Affinity Chromatography and base media support for producing IEX (Ion Exchange) and Hydrophobic interaction chromatography resins.

Product Specifications

Catalog ID R-202
Storage/Handling store at 4°C. Do NOT freeze.
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R-202-5 5 mL $ 64.00
R-202-25 25 mL $ 272.00
R-202-100 100 mL $ 685.00
R-202-500 500 mL $ 2,788.00
R-202-1000 1000 mL $ 5,016.00

Plastic Spin Columns

Catalog ID Size Pricing
P-300 800 μL $ 201.00

Description

No description available

Product Specifications

Catalog ID P-300
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P-300 800 μL $ 201.00

Protein A Agarose Resin (Pharmaceutical grade for research)

Catalog ID Size Pricing
P-400-5 5 mL $ 154.00
P-400-25 25 mL $ 604.00
P-400-50 50 mL $ 1,113.00
P-400-100 100 mL $ 1,907.00

Description

Protein A agarose resin consists of rProtein A covalently bound to cross-linked agarose beads. It provides a very stable bond that can greatly minimize leakage of the Protein A allowing for reuse of the affinity resin in several purification steps. Protein A Agarose resin is available in bulk 5 or 25 ml quantities or in Prepacked 1 and 5 ml columns for immediate gravity flow purifications.

Protein A resin is good for the purification of a wide range of Immunoglobulins of different mammalian species and also certain IgG subclasses that have no other affinity (see additional information).

Protein A is a cell wall component of Staphylococcus aureus. It consists of a single polypeptide chain shaped as a cylinder, which contains five antibody–binding domains. These high affinity regions are specifically bonded to Fc region of the immunoglobulins (specially IgGs of different species).

Product Specifications

Catalog ID P-400
Storage/Handling store at 4°C. Do NOT freeze.
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P-400-5 5 mL $ 154.00
P-400-25 25 mL $ 604.00
P-400-50 50 mL $ 1,113.00
P-400-100 100 mL $ 1,907.00

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