Affinity Reagents

Affinity reagents and affinity-based tools utilize complex networks of specific interactions between various molecules for use in the separation, purification or analysis of proteins. Affinity purification requires an affinity ligand to be coupled to a matrix, which allows capture of the intended product from a complex mixture. Gold Biotechnology’s affinity reagents are activated beads or preactivated resins for use in coupling of affinity ligands and immobilization. Our affinity beads are specifically engineered to meet your specialized needs, whether it be laboratory scale experiments or industry scale separations. GoldBio’s agarose beads are conveniently cross-linked to include any of the following: aminoethyl or glyoxal groups, glutathione S-transferase, Protein A, Protein G, streptavidin and His-tag metal ions such as nickel, cobalt, copper or zinc.

Showing 1-20 of 49 items.

4% HTC Agarose Beads, Standard

Catalog ID Size Pricing
R-200-500 500 mL $ 762.00
R-200-1000 1 L $ 1,076.00

Description

This matrix is a highly cross-linked 4% agarose bead with a bead size of 50-150 µm. It is a gel filtration resin developed for industrial-scale separation of large molecules and virus particles utilizing higher pressure and flow rates rather than low pressure standard beads.

This matrix is not preactivated. For preactivated resins, look for Aminoethyl Agarose Beads, Glyoxal Agarose Beads, Protein A or Protein G.

Product Specifications

Catalog ID R-200
Storage/Handling store at 4°C. Do NOT freeze.
View
Quick Add
R-200-500 500 mL $ 762.00
R-200-1000 1 L $ 1,076.00

5X Phosphate Buffer + 3M Imidazole Solution

Catalog ID Size Pricing
I-905-100 100 mL $ 184.00

Description

This Buffer Set consists of:

- 5X Phosphate Buffer, pH8 (250 mM Phosphate buffer pH 8, 1.5 M NaCl)
- 25 mL 3M Recrystallized Imidazole Solution, pH 6.0

This Buffer set allows researchers to develop, wash and elute buffers for His-tag protein purification.

Product Specifications

Catalog ID I-905
View
Quick Add
I-905-100 100 mL $ 184.00

5X Phosphate Buffer, pH 8

Catalog ID Size Pricing
P-500-50 50 mL $ 61.00
P-500-100 100 mL $ 86.00
P-500-250 250 mL $ 121.00

Description

5X Phosphate Buffer, pH8 (250 mM Phosphate buffer pH 8, 1.5 M NaCl).

Phosphate Buffer, pH8 is a mix of Sodium Phosphate dibasic (Na2HPO4) and Monosodium Phosphate (NaH2PO4). This buffer is the basis for wash and elution buffers for protein purification through His-tag.

Product Specifications

Catalog ID P-500
Storage/Handling Store at 4°C.
View
Quick Add
P-500-50 50 mL $ 61.00
P-500-100 100 mL $ 86.00
P-500-250 250 mL $ 121.00

6% HTC Agarose Beads, Standard

Catalog ID Size Pricing
R-201-500 500 mL $ 826.00
R-201-1000 1 L $ 1,122.00

Description

This matrix is a highly crosslinked 6% agarose bead with a bead size of 50-150 µm. It is a gel filtration resin developed for industrial-scale separation of large molecules and virus particles utilizing higher pressure and flow rates rather than low pressure Standard Beads.

This matrix is not preactivated. For preactivated resins, look for Aminoethyl Agarose Beads, Glyoxal Agarose Beads, Protein A or Protein G.

Product Specifications

Catalog ID R-201
Storage/Handling store at 4°C. Do NOT freeze.
View
Quick Add
R-201-500 500 mL $ 826.00
R-201-1000 1 L $ 1,122.00

Aminoethyl (High Density) 4% Agarose Beads, Crosslinked

There is no image for Aminoethyl (High Density) 4% Agarose Beads, Crosslinked Aminoethyl (High Density) 4% Agarose Beads, Crosslinked
Catalog ID Size Pricing
A-499-25 25 mL $ 151.00
A-499-100 100 mL $ 518.00

Description

High Density Aminoethyl 4% crosslinked beads allows covalent binding of agarose to carboxy group amino acids of the target ligand. This product is adequate to work in batch or column purifications (Low Pressure). The resin has a high amount of aldehyde groups (40-60 µm diaminoethyl / mL gel) providing a very good option to conjugate affinity ligands improving the stability of the ligand. The backbone of the resin is 4% agarose beads and the pore size is adequate to work with enzymes, proteins & antibodies.

Aminoethyl resins give the biomolecules increased stability through the covalent bonds of the agarose to carboxy group amino acids of the target ligand, thus facilitating recovery and later reuse. This covalent binding also confers a qualitative advantage compared to resins activated with CNBr, in which the binding is weaker.

Biomolecules can be immobilized by binding them to a support under conditions that will then determine the characteristics of the complex. Immobilization is a technique that binds a ligand (enzyme, antibody, affinity proteins like Protein A or Protein G) to a support, giving high stability and making it easier to reuse the immobilized molecule.

Product Specifications

Catalog ID A-499
Storage/Handling store at 4°C. Do NOT freeze.
View
Quick Add
A-499-25 25 mL $ 151.00
A-499-100 100 mL $ 518.00

Aminoethyl (High Density) 6% Agarose Beads, Crosslinked

There is no image for Aminoethyl (High Density) 6% Agarose Beads, Crosslinked Aminoethyl (High Density) 6% Agarose Beads, Crosslinked
Catalog ID Size Pricing
A-503-25 25 mL $ 156.00
A-503-100 100 mL $ 573.00

Description

HIGH Density Aminoethyl 6% crosslinked beads allow covalent binding of agarose to carboxy group amino acids of the target ligand. This product is adequate to work in batch or column purifications (Low Pressure). The resin has a high amount of aldehyde groups (40-60 µm diaminoethyl / mL gel) providing a very good option to conjugate affinity ligands improving the stability of the ligand. The backbone of the resin is 6% agarose beads and the pore size is adequate to work with enzymes & proteins mainly.

Aminoethyl resins give the biomolecules increased stability through the covalent bonds of the agarose to carboxy group amino acids of the target ligand, thus facilitating recovery and later reuse. This covalent binding also confers a qualitative advantage compared to resins activated with CNBr, in which the binding is weaker.

Biomolecules can be immobilized by binding them to a support under conditions that will then determine the characteristics of the complex. Immobilization is a technique that binds a ligand (enzyme, antibody, affinity proteins like Protein A or Protein G) to a support, giving high stability and making it easier to reuse the immobilized molecule.

Product Specifications

Catalog ID A-503
Storage/Handling store at 4°C. Do NOT freeze.
View
Quick Add
A-503-25 25 mL $ 156.00
A-503-100 100 mL $ 573.00

Aminoethyl (Low Density) 6% Agarose Beads, Crosslinked

Catalog ID Size Pricing
A-501-25 25 mL $ 151.00
A-501-100 100 mL $ 498.00

Description

LOW Density Aminoethyl 6% crosslinked beads allow covalent binding of agarose to carboxy group amino acids of the target ligand. This product is adequate to work in batch or column purifications (Low Pressure). This resin has a low content of aldehyde groups (15- 25 µm diaminoethyl / ml gel) providing a very good option to conjugate affinity ligands without distorting the target ligand. The backbone of the resin is 6% agarose beads and the pore size is adequate to work with enzymes & proteins mainly.

Aminoethyl resin give the biomolecules increased stability through the covalent bonds of the agarose to carboxy group amino acids of the target ligand, thus facilitating recovery and later reuse. This covalent binding also confers a qualitative advantage compared to resins activated with CNBr, in which the binding is weaker.

Product Specifications

Catalog ID A-501
Storage/Handling store at 4°C. Do NOT freeze.
View
Quick Add
A-501-25 25 mL $ 151.00
A-501-100 100 mL $ 498.00

Aminoethyl (Very Low Density) 4% Agarose Beads, Crosslinked

Catalog ID Size Pricing
A-500-25 25 mL $ 144.00
A-500-100 100 mL $ 491.00

Description

Biomolecules can be immobilized by binding them to a support under conditions that will then determine the characteristics of the complex. Immobilization is a technique that binds a ligand (enzyme, antibody, affinity proteins like Protein A or Protein G) to a support, giving high stability and making it easier to reuse the immobilized molecule.

Aminoethyl resin give the biomolecules increased stability through the covalent bonds of the agarose to carboxy group amino acids of the target ligand, thus facilitating recovery and later reuse. This covalent binding also confers a qualitative advantage compared to resins activated with CNBr, in which the binding is weaker.

The choice of Glyoxal or Aminoethyl will depend on the biomolecule to be immobilized, the accessibility of the reactive groups, and the direction/ orientation required for the binding to the support.

Product Specifications

Catalog ID A-500
Storage/Handling store at 4°C. Do NOT freeze.
View
Quick Add
A-500-25 25 mL $ 144.00
A-500-100 100 mL $ 491.00

Aminoethyl HTC 4% HIGH Density Agarose Beads

There is no image for Aminoethyl HTC 4% HIGH Density Agarose Beads Aminoethyl HTC 4% HIGH Density Agarose Beads
Catalog ID Size Pricing
A-504-25 25 mL $ 165.00
A-504-100 100 mL $ 568.00

Description

Aminoethyl HTC 4% HIGH Density beads allow covalent binding of agarose to carboxy group amino acids of the target ligand. This product is adequate to work in large scale process (High Pressure). The resin has a high amount of amino groups (40-60 µm diaminoethyl / mL gel) providing multiple binding points and high immobilized ligand stability. The resin has been manufactured using 4% HTC agarose beads, and the pore size is adequate to work with enzymes, proteins & antibodies. The stability at high flow is adequate for large scale process.

HTC beads are high throughput beads designed to meet the demand for industrial process separation. Their rigidity and mechanical resistance permits high flow rates with good resolution in a minimum time frame, making these beads ideal for process-scale use. HTC beads are highly crosslinked 4% and 6% agarose matrices and have excellent physical and chromatographic qualities. They are autoclavable. These beads are an ideal support for the immobilization of ligands for Affinity Chromatography and base media support for producing IEX (Ion Exchange) and hydrophobic interaction chromatography resins.

Product Specifications

Catalog ID A-504
Storage/Handling store at 4°C. Do NOT freeze.
View
Quick Add
A-504-25 25 mL $ 165.00
A-504-100 100 mL $ 568.00

Aminoethyl HTC 4% VERY LOW Density Agarose Beads

There is no image for Aminoethyl HTC 4% VERY LOW Density Agarose Beads Aminoethyl HTC 4% VERY LOW Density Agarose Beads
Catalog ID Size Pricing
A-505-25 25 mL $ 158.00
A-505-100 100 mL $ 540.00

Description

Aminoethyl HTC 4% VERY LOW Density beads allow a covalent binding of agarose to carboxy group amino acids of the target ligand. This product is adequate to work in large scale process (High Pressure). The resin has a low content of amino groups (3- 6 µm diaminoethyl / mL gel) providing a minimum distortion of conjugated affinity ligand. The resin has been manufactured using 4% HTC agarose beads, and the pore size is adequate to work with enzymes, proteins & antibodies. The stability at high flow is adequate for large scale process.

HTC beads are high throughput beads designed to meet the demand for industrial process separation. Their rigidity and mechanical resistance permits high flow rates with good resolution in a minimum time frame, making these beads ideal for process-scale use. HTC beads are highly crosslinked 4% and 6% agarose matrices and have excellent physical and chromatographic qualities. They are autoclavable. These beads are an ideal support for the immobilization of ligands for Affinity Chromatography and base media support for producing IEX (Ion Exchange) and hydrophobic interaction chromatography resins.

Product Specifications

Catalog ID A-505
Storage/Handling store at 4°C. Do NOT freeze.
View
Quick Add
A-505-25 25 mL $ 158.00
A-505-100 100 mL $ 540.00

Aminoethyl HTC 6% HIGH Density Agarose Beads

There is no image for Aminoethyl HTC 6% HIGH Density Agarose Beads Aminoethyl HTC 6% HIGH Density Agarose Beads
Catalog ID Size Pricing
A-507-25 25 mL $ 173.00
A-507-100 100 mL $ 573.00

Description

Aminoethyl 6% HIGH Density beads allow covalent binding of agarose to carboxy group amino acids of the target ligand. This product is adequate to work in large scale process (High Pressure). The resin has a high amount of amino groups (40-60 µm diaminoethyl / mL gel) providing multiple binding points and high immobilized ligand stability. The resin has been manufactured using 6% HTC agarose beads, and the pore size is adequate to work with enzymes, proteins & small ligands. The stability at high flow is adequate to large scale process.

HTC beads are high throughput beads designed to meet the demand for industrial process separation. Their rigidity and mechanical resistance permits high flow rates with good resolution in a minimum time frame, making these beads ideal for process-scale use. HTC beads are highly crosslinked 4% and 6% agarose matrices and have excellent physical and chromatographic qualities. They are autoclavable. These beads are an ideal support for the immobilization of ligands for Affinity Chromatography and base media support for producing IEX (Ion Exchange) and hydrophobic interaction chromatography resins.

Product Specifications

Catalog ID A-507
Storage/Handling store at 4°C. Do NOT freeze.
View
Quick Add
A-507-25 25 mL $ 173.00
A-507-100 100 mL $ 573.00

Aminoethyl HTC 6% LOW Density Agarose Beads

There is no image for Aminoethyl HTC 6% LOW Density Agarose Beads Aminoethyl HTC 6% LOW Density Agarose Beads
Catalog ID Size Pricing
A-506-25 25 mL $ 164.00
A-506-100 100 mL $ 493.00

Description

Aminoethyl HTC 6% LOW Density beads allow covalent binding of agarose to carboxy group amino acids of the target ligand. This product is adequate to work in large scale process (High Pressure). The resin has a low content of amino groups (15- 25 µm diaminoethyl / mL gel) providing a minimum distortion of conjugated affinity ligand. The resin has been manufactured using 6% HTC agarose beads and the pore size is adequate to work with enzymes, proteins & small ligands. The stability at high flow is adequate for large scale process.

HTC beads are high throughput beads designed to meet the demand for industrial process separation. Their rigidity and mechanical resistance permits high flow rates with good resolution in a minimum time frame, making these beads ideal for process-scale use. HTC beads are highly crosslinked 4% and 6% agarose matrices and have excellent physical and chromatographic qualities. They are autoclavable. These beads are an ideal support for the immobilization of ligands for Affinity Chromatography and base media support for producing IEX (Ion Exchange) and hydrophobic interaction chromatography resins.

Product Specifications

Catalog ID A-506
Storage/Handling store at 4°C. Do NOT freeze.
View
Quick Add
A-506-25 25 mL $ 164.00
A-506-100 100 mL $ 493.00

Aminoethyl HTC 6% VERY LOW Density Agarose Beads

There is no image for Aminoethyl HTC 6% VERY LOW Density Agarose Beads Aminoethyl HTC 6% VERY LOW Density Agarose Beads
Catalog ID Size Pricing
A-508-25 25 mL $ 176.00
A-508-100 100 mL $ 597.00

Description

Aminoethyl HTC 6% VERY LOW Density beads allow covalent binding of agarose to carboxy group amino acids of the target ligand. The resin has a low content of amino groups (3- 6 µm diaminoethyl / mL gel) providing a minimum distortion of conjugated affinity ligand. The resin has been manufactured using 6% HTC FINE agarose beads, and the pore size is adequate to work with enzymes, proteins & small ligands. The bead size is adequate to manufacture resins for packing cartridges.

HTC beads are high throughput beads designed to meet the demand for industrial process separation. Their rigidity and mechanical resistance permits high flow rates with good resolution in a minimum time frame, making these beads ideal for process-scale use. HTC beads are highly crosslinked 4% and 6% agarose matrices and have excellent physical and chromatographic qualities. They are autoclavable. These beads are an ideal support for the immobilization of ligands for Affinity Chromatography and base media support for producing IEX (Ion Exchange) and hydrophobic interaction chromatography resins.

Product Specifications

Catalog ID A-508
Storage/Handling store at 4°C. Do NOT freeze.
View
Quick Add
A-508-25 25 mL $ 176.00
A-508-100 100 mL $ 597.00

Cobalt Agarose Beads (High Density)

Catalog ID Size Pricing
H-310-5 5 mL $ 48.00
H-310-10 10 mL $ 83.00
H-310-25 25 mL $ 188.00
H-310-50 50 mL $ 313.00
H-310-100 100 mL $ 560.00
H-310-500 500 mL $ 2,294.00

Description

Available in bulk or in prepacked columns, cobalt has a binding capacity of >25 mg/ml with approximately 60 kDa protein. Cobalt binds fewer host protein contaminants, resulting in lower background than nickel resins and no metal contamination in eluted histidine-tagged protein sample. In addition, it allows for purification of proteins under native or denaturing conditions. Cobalt Agarose works very well with Ni/Cu/Co Resin Wash Buffer and Histidine Protein Elution Buffer.

Product Specifications

Catalog ID H-310
Storage/Handling store at 4°C. Do NOT freeze.
View
Quick Add
H-310-5 5 mL $ 48.00
H-310-10 10 mL $ 83.00
H-310-25 25 mL $ 188.00
H-310-50 50 mL $ 313.00
H-310-100 100 mL $ 560.00
H-310-500 500 mL $ 2,294.00

Cobalt HTC Agarose Beads

Catalog ID Size Pricing
R-203-25 25 mL $ 281.00
R-203-100 100 mL $ 706.00
R-203-500 500 mL $ 2,872.00

Description

Cobalt HTC 6% Agarose Beads are suitable for downstream purification using IMAC. These resins combine the advantages of Cobalt purification with the high flow rates of the HTC resin (70% higher than commercial standards).

Product Specifications

Catalog ID R-203
Storage/Handling store at 4°C. Do NOT freeze.
View
Quick Add
R-203-25 25 mL $ 281.00
R-203-100 100 mL $ 706.00
R-203-500 500 mL $ 2,872.00

Copper Agarose Beads (Low Density)

Catalog ID Size Pricing
H-311-25 25 mL $ 225.00
H-311-100 100 mL $ 595.00

Description

Low Density Copper Agarose Resin is available in bulk or in prepacked columns. Purification of proteins is a vital part of modern research. Impure extracts generally contain a wide range of proteins with diverse biological functions and different chemistry, which need to be separated.

IDA cross-linked Agarose resin consists of iminodiacetic acid groups ligated by stable ether linkages via a spacer arm. IDA is a tridentate chelating agent, covalently coupled to cross-linked agarose beads. This resin is loaded with Cu2+. The resulting resin (ready-to-use) is ideal for rapid purification of His-tagged proteins.

Affinity Chromatography is based on the interaction between certain superficial protein residues (histidines, cysteines and to a lesser extent tryptophans), with transition metal cations, forming chelates.

Product Specifications

Catalog ID H-311
Storage/Handling store at 4°C. Do NOT freeze.
View
Quick Add
H-311-25 25 mL $ 225.00
H-311-100 100 mL $ 595.00

Glutathione Agarose Resin

Catalog ID Size Pricing
G-250-5 5 mL $ 89.00
G-250-10 10 mL $ 166.00
G-250-50 50 mL $ 641.00
G-250-100 100 mL $ 996.00

Description

Glutathione has been covalently linked for use in affinity purification of glutathione-S-transferase (GST) and GST fusion proteins.

Product Specifications

Catalog ID G-250
Storage/Handling store at 4°C. Do NOT freeze.
View
Quick Add
G-250-5 5 mL $ 89.00
G-250-10 10 mL $ 166.00
G-250-50 50 mL $ 641.00
G-250-100 100 mL $ 996.00

Glyoxal (High Density) 4% Agarose Beads, Crosslinked

Catalog ID Size Pricing
G-301-25 25 mL $ 165.00
G-301-100 100 mL $ 499.00

Description

Biomolecules can be immobilized by binding them to a support under conditions that will then determine the characteristics of the complex. Immobilization is a technique that binds a ligand (enzyme, antibody, affinity proteins like Protein A or Protein G) to a support, giving high stability and making it easier to reuse the immobilized molecule.

Glyoxal beads allow a covalent binding of agarose to lysine amino groups (similar orientation to CNBr). Glyoxal agarose beads are ready to use and require no activation. The resin has a high amount of aldehydes groups (40-60 µmoles Glyoxal / ml gel) providing a very good option to conjugate affinity ligands improving the stability of the ligand.

The choice of Glyoxal or Aminoethyl will depend on the biomolecule to be immobilized, the accessibility of the reactive groups and the direction/ orientation required for the binding to the support.

Product Specifications

Catalog ID G-301
Storage/Handling store at 4°C. Do NOT freeze.
View
Quick Add
G-301-25 25 mL $ 165.00
G-301-100 100 mL $ 499.00

Glyoxal (High Density) 6% Agarose Beads, Crosslinked

Catalog ID Size Pricing
G-303-25 25 mL $ 169.00
G-303-100 100 mL $ 506.00

Description

Biomolecules can be immobilized by binding them to a support under conditions that will then determine the characteristics of the complex. Immobilization is a technique that binds a ligand (enzyme, antibody, affinity proteins like Protein A or Protein G) to a support, giving high stability and making it easier to reuse the immobilized molecule.

Glyoxal beads allow a covalent binding of agarose to lysine amino groups (similar orientation to CNBr). Glyoxal agarose beads are ready to use and require no activation. The resin has a high amount of aldehydes groups (40-60 µmoles Glyoxal / ml gel) providing a very good option to conjugate affinity ligands improving the stability of the ligand.

The choice of Glyoxal or Aminoethyl will depend on the biomolecule to be immobilized, the accessibility of the reactive groups, and the direction/ orientation required for the binding to the support.

Product Specifications

Catalog ID G-303
Storage/Handling store at 4°C. Do NOT freeze.
View
Quick Add
G-303-25 25 mL $ 169.00
G-303-100 100 mL $ 506.00

Glyoxal (Low Density) 4% Agarose Beads, Crosslinked

Catalog ID Size Pricing
G-300-25 25 mL $ 174.00
G-300-100 100 mL $ 392.00

Description

Biomolecules can be immobilized by binding them to a support under conditions that will then determine the characteristics of the complex. Immobilization is a technique that binds a ligand (enzyme, antibody, affinity proteins like Protein A or Protein G) to a support, giving high stability and making it easier to reuse the immobilized molecule.

Glyoxal beads allow a covalent binding of agarose to lysine amino groups (similar orientation to CNBr). Glyoxal agarose beads are ready to use and require no activation. The resin has a high amount of aldehydes groups (15-25 µmoles Glyoxal / ml gel) providing a very good option to conjugate affinity ligands improving the stability of the ligand.

The choice of Glyoxal or Aminoethyl will depend on the biomolecule to be immobilized, the accessibility of the reactive groups, and the direction/ orientation required for the binding to the support.

Product Specifications

Catalog ID G-300
Storage/Handling store at 4°C. Do NOT freeze.
View
Quick Add
G-300-25 25 mL $ 174.00
G-300-100 100 mL $ 392.00

Join our list to receive promos and articles.

Join Now