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    Affinity reagents and affinity-based tools utilize complex networks of specific interactions between various molecules for use in the separation, purification or analysis of proteins. Affinity purification requires an affinity ligand to be coupled to a matrix, which allows capture of the intended product from a complex mixture. Gold Biotechnology’s affinity reagents are activated beads or preactivated resins for use in coupling of affinity ligands and immobilization. Our affinity beads are specifically engineered to meet your specialized needs, whether it be laboratory scale experiments or industry scale separations. GoldBio’s agarose beads are conveniently cross-linked to include any of the following: aminoethyl or glyoxal groups, glutathione S-transferase, Protein A, Protein G, streptavidin and His-tag metal ions such as nickel, cobalt, copper or zinc.

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    Affinity reagents and affinity-based tools utilize complex networks of specific interactions between various molecules for use in the separation, purification or analysis of proteins. Affinity purification requires an affinity ligand to be coupled to a matrix, which allows capture of the intended product from a complex mixture. Gold Biotechnology’s affinity reagents are activated beads or preactivated resins for use in coupling of affinity ligands and immobilization. Our affinity beads are specifically engineered to meet your specialized needs, whether it be laboratory scale experiments or industry scale separations. GoldBio’s agarose beads are conveniently cross-linked to include any of the following: aminoethyl or glyoxal groups, glutathione S-transferase, Protein A, Protein G, streptavidin and His-tag metal ions such as nickel, cobalt, copper or zinc.

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  • object(anvilstl\commercio\models\Category)#440 (15) { ["featured_image_urls"]=> array(0) { } ["featured_image"]=> array(0) { } ["banner_image_urls"]=> array(0) { } ["productCount"]=> NULL ["_attributes":"yii\db\BaseActiveRecord":private]=> array(23) { ["id"]=> int(8) ["root"]=> int(1) ["lft"]=> int(74) ["rgt"]=> int(89) ["level"]=> int(3) ["name"]=> string(20) "His-tag Purification" ["url_name"]=> string(20) "his-tag-purification" ["title"]=> NULL ["short_description"]=> string(0) "" ["long_description"]=> string(834) "His-tag purification is a vital part of modern research. Impure extracts generally contain a wide range of proteins with diverse biological functions and chemistry that need to be separated. GoldBio's IDA cross-linked agarose resins consist of iminodiacetic acid groups ligated by stable ether linkages via a spacer arm. IDA is a tridentate chelating agent, covalently coupled to cross-linked agarose beads. These resins are loaded with a divalent metal ions (such as nickel, cobalt, zinc or copper) in either high density or low density affinities to meet your specific research requirements. Copper ions have high affinity (binding strength) but low specificity (selectivity); cobalt ions have low affinity but have high specificity; while nickel and zinc combine a mid-ranged affinity and specificity for most general purposes.
    " ["hide"]=> int(0) ["third_party_id"]=> int(156) ["featured_image_id"]=> int(0) ["banner_image_id"]=> int(0) ["meta_description"]=> string(154) "His-tag purification is vital within biology, and GoldBio’s cross-linked agarose resins are loaded with divalent metal ions to meet your research needs." ["meta_keywords"]=> string(0) "" ["root_sort"]=> int(0) ["type"]=> string(7) "product" ["condition_type"]=> int(1) ["condition_match_type"]=> int(1) ["sort_type"]=> int(1) ["items_per_page"]=> int(25) ["color"]=> NULL } ["_oldAttributes":"yii\db\BaseActiveRecord":private]=> array(23) { ["id"]=> int(8) ["root"]=> int(1) ["lft"]=> int(74) ["rgt"]=> int(89) ["level"]=> int(3) ["name"]=> string(20) "His-tag Purification" ["url_name"]=> string(20) "his-tag-purification" ["title"]=> NULL ["short_description"]=> string(0) "" ["long_description"]=> string(834) "His-tag purification is a vital part of modern research. Impure extracts generally contain a wide range of proteins with diverse biological functions and chemistry that need to be separated. GoldBio's IDA cross-linked agarose resins consist of iminodiacetic acid groups ligated by stable ether linkages via a spacer arm. IDA is a tridentate chelating agent, covalently coupled to cross-linked agarose beads. These resins are loaded with a divalent metal ions (such as nickel, cobalt, zinc or copper) in either high density or low density affinities to meet your specific research requirements. Copper ions have high affinity (binding strength) but low specificity (selectivity); cobalt ions have low affinity but have high specificity; while nickel and zinc combine a mid-ranged affinity and specificity for most general purposes.
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  • object(anvilstl\commercio\models\Category)#442 (15) { ["featured_image_urls"]=> array(0) { } ["featured_image"]=> array(0) { } ["banner_image_urls"]=> array(0) { } ["productCount"]=> NULL ["_attributes":"yii\db\BaseActiveRecord":private]=> array(23) { ["id"]=> int(9) ["root"]=> int(1) ["lft"]=> int(75) ["rgt"]=> int(76) ["level"]=> int(4) ["name"]=> string(18) "Buffers and Washes" ["url_name"]=> string(23) "buffers-washes-reagents" ["title"]=> NULL ["short_description"]=> string(0) "" ["long_description"]=> string(507) "GoldBio offers various buffers for your affinity purification demands. We provide basic washing and elution buffers, already prepared and ready to use, as well as a His-tag buffer set which provides all the buffers necessary for purification. GoldBio’s recrystallized imidazole, used for eluting His-tagged proteins in protein purification, has the highest purity available anywhere on the market and is available in a prepared, ready solution or in dry form to create your lab preferred concentrations." 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    Buffers are essential in many different biological and biotechnical applications. GoldBio’s vast array of application-specific buffers include biological, electrophoretic and lysis buffers in order to ensure that we have exactly the right product for both your research needs as well as your budget.

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    Buffers are essential in many different biological and biotechnical applications. GoldBio’s vast array of application-specific buffers include biological, electrophoretic and lysis buffers in order to ensure that we have exactly the right product for both your research needs as well as your budget.

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  • object(anvilstl\commercio\models\Category)#446 (15) { ["featured_image_urls"]=> array(0) { } ["featured_image"]=> array(0) { } ["banner_image_urls"]=> array(0) { } ["productCount"]=> NULL ["_attributes":"yii\db\BaseActiveRecord":private]=> array(23) { ["id"]=> int(197) ["root"]=> int(393) ["lft"]=> int(28) ["rgt"]=> int(29) ["level"]=> int(1) ["name"]=> string(39) "Protein Expression & Purification (old)" ["url_name"]=> string(32) "protein-expression-purification2" ["title"]=> NULL ["short_description"]=> string(0) "" ["long_description"]=> string(484) "

    Proteins are fundamental macromolecules found in every biological system and form the basis of many fields of work from research into drug creation to industrial applications like fermentation. GoldBio protein products are ideal for every step of your protein synthesis. Our reagents and tools are of the highest quality available at a fraction of the prices of other vendors, so you can be assured that your research yields confident results without compromising your budget.

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    Proteins are fundamental macromolecules found in every biological system and form the basis of many fields of work from research into drug creation to industrial applications like fermentation. GoldBio protein products are ideal for every step of your protein synthesis. Our reagents and tools are of the highest quality available at a fraction of the prices of other vendors, so you can be assured that your research yields confident results without compromising your budget.

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  • object(anvilstl\commercio\models\Category)#450 (15) { ["featured_image_urls"]=> array(3) { ["small"]=> string(83) "https://s3.amazonaws.com/commercio/goldbio-2018/collection/small/Agarose-Resins.jpg" ["medium"]=> string(84) "https://s3.amazonaws.com/commercio/goldbio-2018/collection/medium/Agarose-Resins.jpg" ["large"]=> string(83) "https://s3.amazonaws.com/commercio/goldbio-2018/collection/large/Agarose-Resins.jpg" } ["featured_image"]=> array(3) { ["small"]=> string(83) "https://s3.amazonaws.com/commercio/goldbio-2018/collection/small/Agarose-Resins.jpg" ["medium"]=> string(84) "https://s3.amazonaws.com/commercio/goldbio-2018/collection/medium/Agarose-Resins.jpg" ["large"]=> string(83) "https://s3.amazonaws.com/commercio/goldbio-2018/collection/large/Agarose-Resins.jpg" } ["banner_image_urls"]=> array(0) { } ["productCount"]=> NULL ["_attributes":"yii\db\BaseActiveRecord":private]=> array(23) { ["id"]=> int(242) ["root"]=> int(393) ["lft"]=> int(54) ["rgt"]=> int(55) ["level"]=> int(1) ["name"]=> string(20) "Agarose Resins (old)" ["url_name"]=> string(15) "agarose-resins2" ["title"]=> NULL ["short_description"]=> string(0) "" ["long_description"]=> string(846) "

    Agarose resins possess physical characteristics which make them a cornerstone in the analytical field due to their ability to separate and purify molecules depending on the application. These resins can be used to manipulate proteins and amino acid binding to suit desirable outcomes. In His-Tag and size-exclusion chromatography, resins are used to help isolate specific proteins from undesirable ones for further applications. GST chromatography resins can be used to bind together DNA coding sequences. Protein A & G resins are also help bind immunoglobulins. For industrial scale applications, high-throughput chromatography resin allows for high flow rates without compromising binding capacity. GoldBio provides these high-quality chromatography products and the necessary equipment to design and perform separations for a low cost.

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    Agarose resins possess physical characteristics which make them a cornerstone in the analytical field due to their ability to separate and purify molecules depending on the application. These resins can be used to manipulate proteins and amino acid binding to suit desirable outcomes. In His-Tag and size-exclusion chromatography, resins are used to help isolate specific proteins from undesirable ones for further applications. GST chromatography resins can be used to bind together DNA coding sequences. Protein A & G resins are also help bind immunoglobulins. For industrial scale applications, high-throughput chromatography resin allows for high flow rates without compromising binding capacity. GoldBio provides these high-quality chromatography products and the necessary equipment to design and perform separations for a low cost.

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  • object(anvilstl\commercio\models\Category)#452 (15) { ["featured_image_urls"]=> array(0) { } ["featured_image"]=> array(0) { } ["banner_image_urls"]=> array(0) { } ["productCount"]=> NULL ["_attributes":"yii\db\BaseActiveRecord":private]=> array(23) { ["id"]=> int(267) ["root"]=> int(1) ["lft"]=> int(190) ["rgt"]=> int(191) ["level"]=> int(3) ["name"]=> string(35) "Buffers & Washes for Chromatography" ["url_name"]=> string(33) "buffers-washes-for-chromatography" ["title"]=> NULL ["short_description"]=> string(0) "" ["long_description"]=> string(481) "Buffers and washes are important for purification of proteins in a chromatography system. In chromatography, the primary buffers help the proteins bind with ligands present in the matrix. To purify this sample an elution buffer, or wash, is used to separate out unwanted proteins. It is important for both types of buffers to be of high quality to ensure the maximum purity of your sample, and GoldBio provides a variety of buffers and washes at affordable prices." ["hide"]=> int(0) ["third_party_id"]=> NULL ["featured_image_id"]=> int(0) ["banner_image_id"]=> int(0) ["meta_description"]=> string(124) "GoldBio’s washes and buffers are of the highest quality available, ensuring maximum purity of your sample, for a low cost." 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    Separating proteins from cellular components is critical when trying to determine their overall structure and function. GoldBio carries products designed for the whole range of chromatography applications, from gel chromatography to more targeted applications like affinity, ion exchange or polyhistidine-tag binding. When it comes to purification, the higher the quality of materials, the better the results. GoldBio agaroses come in different densities, with high throughput beads designed for industrial applications. Our Cobalt and Nickel agarose beads are perfect for your his-tag recombinant protein recovery, and our Ultra HBCTM streptavidin resin has superior coupling chemistry and higher binding performance than other vendors. GoldBio offers quality products, at affordable prices.

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    Separating proteins from cellular components is critical when trying to determine their overall structure and function. GoldBio carries products designed for the whole range of chromatography applications, from gel chromatography to more targeted applications like affinity, ion exchange or polyhistidine-tag binding. When it comes to purification, the higher the quality of materials, the better the results. GoldBio agaroses come in different densities, with high throughput beads designed for industrial applications. Our Cobalt and Nickel agarose beads are perfect for your his-tag recombinant protein recovery, and our Ultra HBCTM streptavidin resin has superior coupling chemistry and higher binding performance than other vendors. GoldBio offers quality products, at affordable prices.

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    • Imidazole, Recrystallized

    {{ i.item.name }}
    Specifications Documentation Citations Related Products
    1
    Request Lot Specific COA Citation Search Tool
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    Product Description

    Imidazole is a planar 5-membered ring. It is a highly polar, highly water soluble amphoteric compound. Imidazole is often used to elute tagged proteins bound to metal ions attached to the surface of beads in a chromatography column by displacing the His-tag from the metal ion and freeing the His-tagged proteins. Imidazole is also commonly used as a buffer for enzymatic reactions and has a buffering pH range of 6.2 - 7.8.

    Recrystallized Imidazole from GoldBio is specially purified Imidazole designed for histidine tagged protein purification. We purify the Imidazole at Gold Biotechnology’s labs and go through multiple purification steps including ultrafiltration and recrystallization to obtain the most purified Imidazole on the market. The final Imidazole product is tested in our labs for elution of tagged protein from Ni columns.

    Product Specifications:
    Sterile filtered and recrystallized Imidazole;
    Glyoxaline;
    1,3-diazacyclopenta-2,4-diene

    Molecular Biology Grade
    Protease Free

    Formula: C3H4N2

    MW: 68.08 g/mol

    Ships in 25 g units

    PubChem Chemical ID: 795

    Product Specifications

    Catalog ID I-902
    CAS # 288-32-4
    MW 68.08 g/mol
    Storage/Handling Store at room temperature.
    UN Number 3262
    Item Class 8
    Group Number III

    Technical Documentation

    P Protocol
    O Other
    C Certificate of Analysis
    S Safety Data Sheet
    P
    GoldBio His-Tag Column Prep Protocol

    Quick and Simple His-Tag Column Prep protocol including Pouring and Packing an Agarose Column
    P
    IPTG Induction and Extraction of Proteins Protocol

    IPTG Induction & Extraction of Proteins - by Arur and Nayak. Schedl Lab, Wash U. St. Louis
    P
    Enzymatic Assay of L-Fucose Dehydrogenase

    Enzymatic Assay of L-Fucose Dehyrogenase
    P
    Affinity His-Tag Purification utilizing Nickel NTA Magnetic Agarose Beads

    Protocol for the use of Nickel NTA magnetic agarose beads in the purification of recombinant proteins labeled with histidine residues
    O
    Guide: Commonly used Buffers

    Guide: Commonly used Buffers contains name, pH range, applications, composition and other characteristics of buffers commonly used in the lab.

    C
    Sample COA - I-902

    Sample Certificate of Analysis - Imidazole, Recrystallized
    COA for example only. Actual tests may differ.
    S
    SDS (MSDS) - Recrystallized Imidazole

    Safety Data Sheet

    Citations

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