Affinity reagents and affinity-based tools utilize complex networks of specific interactions between various molecules for use in the separation, purification or analysis of proteins. Affinity purification requires an affinity ligand to be coupled to a matrix, which allows capture of the intended product from a complex mixture. Gold Biotechnology’s affinity reagents are activated beads or preactivated resins for use in coupling of affinity ligands and immobilization. Our affinity beads are specifically engineered to meet your specialized needs, whether it be laboratory scale experiments or industry scale separations. GoldBio’s agarose beads are conveniently cross-linked to include any of the following: aminoethyl or glyoxal groups, glutathione S-transferase, Protein A, Protein G, streptavidin and His-tag metal ions such as nickel, cobalt, copper or zinc.
Affinity reagents and affinity-based tools utilize complex networks of specific interactions between various molecules for use in the separation, purification or analysis of proteins. Affinity purification requires an affinity ligand to be coupled to a matrix, which allows capture of the intended product from a complex mixture. Gold Biotechnology’s affinity reagents are activated beads or preactivated resins for use in coupling of affinity ligands and immobilization. Our affinity beads are specifically engineered to meet your specialized needs, whether it be laboratory scale experiments or industry scale separations. GoldBio’s agarose beads are conveniently cross-linked to include any of the following: aminoethyl or glyoxal groups, glutathione S-transferase, Protein A, Protein G, streptavidin and His-tag metal ions such as nickel, cobalt, copper or zinc.
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Buffers are essential in many different biological and biotechnical applications. GoldBio’s vast array of application-specific buffers include biological, electrophoretic and lysis buffers in order to ensure that we have exactly the right product for both your research needs as well as your budget.
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the basis of many fields of work from research into drug creation to industrial applications like fermentation. GoldBio protein products are ideal for every step of your protein synthesis. Our reagents and tools are of the highest quality available at a fraction of the prices of other vendors, so you can be assured that your research yields confident results without compromising your budget.
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NULL } } } ["_eventWildcards":"yii\base\Component":private]=> array(0) { } ["_behaviors":"yii\base\Component":private]=> array(1) { ["tree"]=> object(creocoder\nestedsets\NestedSetsBehavior)#447 (8) { ["treeAttribute"]=> string(4) "root" ["leftAttribute"]=> string(3) "lft" ["rightAttribute"]=> string(3) "rgt" ["depthAttribute"]=> string(5) "level" ["operation":protected]=> NULL ["node":protected]=> NULL ["owner"]=> *RECURSION* ["_attachedEvents":"yii\base\Behavior":private]=> array(6) { ["beforeInsert"]=> string(12) "beforeInsert" ["afterInsert"]=> string(11) "afterInsert" ["beforeUpdate"]=> string(12) "beforeUpdate" ["afterUpdate"]=> string(11) "afterUpdate" ["beforeDelete"]=> string(12) "beforeDelete" ["afterDelete"]=> string(11) "afterDelete" } } } ["__fieldGroups":protected]=> array(0) { } }Agarose resins possess physical characteristics which make them a cornerstone in the analytical field due to their ability to separate and purify molecules depending on the application. These resins can be used to manipulate proteins and amino acid binding to suit desirable outcomes. In His-Tag and size-exclusion chromatography, resins are used to help isolate specific proteins from undesirable ones for further applications. GST chromatography resins can be used to bind together DNA coding sequences. Protein A & G resins are also help bind immunoglobulins. For industrial scale applications, high-throughput chromatography resin allows for high flow rates without compromising binding capacity. GoldBio provides these high-quality chromatography products and the necessary equipment to design and perform separations for a low cost.
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their overall structure and function. GoldBio carries products designed for the whole range of chromatography applications, from gel chromatography to more targeted applications like affinity, ion exchange or polyhistidine-tag binding. When it comes to purification, the higher the quality of materials, the better the results. GoldBio agaroses come in different densities, with high throughput beads designed for industrial applications. Our Cobalt and Nickel agarose beads are perfect for your his-tag recombinant protein recovery, and our Ultra HBCTM streptavidin resin has superior coupling chemistry and higher binding performance than other vendors. GoldBio offers quality products, at affordable prices.
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"afterDelete" } [1]=> NULL } } } ["_eventWildcards":"yii\base\Component":private]=> array(0) { } ["_behaviors":"yii\base\Component":private]=> array(1) { ["tree"]=> object(creocoder\nestedsets\NestedSetsBehavior)#455 (8) { ["treeAttribute"]=> string(4) "root" ["leftAttribute"]=> string(3) "lft" ["rightAttribute"]=> string(3) "rgt" ["depthAttribute"]=> string(5) "level" ["operation":protected]=> NULL ["node":protected]=> NULL ["owner"]=> *RECURSION* ["_attachedEvents":"yii\base\Behavior":private]=> array(6) { ["beforeInsert"]=> string(12) "beforeInsert" ["afterInsert"]=> string(11) "afterInsert" ["beforeUpdate"]=> string(12) "beforeUpdate" ["afterUpdate"]=> string(11) "afterUpdate" ["beforeDelete"]=> string(12) "beforeDelete" ["afterDelete"]=> string(11) "afterDelete" } } } ["__fieldGroups":protected]=> array(0) { } }Imidazole, Recrystallized
Product Description
Imidazole is a planar 5-membered ring. It is a highly polar, highly water soluble amphoteric compound. Imidazole is often used to elute tagged proteins bound to metal ions attached to the surface of beads in a chromatography column by displacing the His-tag from the metal ion and freeing the His-tagged proteins. Imidazole is also commonly used as a buffer for enzymatic reactions and has a buffering pH range of 6.2 - 7.8.
Recrystallized Imidazole from GoldBio is specially purified Imidazole designed for histidine tagged protein purification. We purify the Imidazole at Gold Biotechnology’s labs and go through multiple purification steps including ultrafiltration and recrystallization to obtain the most purified Imidazole on the market. The final Imidazole product is tested in our labs for elution of tagged protein from Ni columns.
Product Specifications:
Sterile filtered and recrystallized Imidazole;
Glyoxaline;
1,3-diazacyclopenta-2,4-diene
Molecular Biology Grade
Protease Free
Formula: C3H4N2
MW: 68.08 g/mol
Ships in 25 g units
PubChem Chemical ID: 795
Product Specifications
Catalog ID | I-902 |
---|---|
CAS # | 288-32-4 |
MW | 68.08 g/mol |
Storage/Handling | Store at room temperature. |
UN Number | 3262 |
Item Class | 8 |
Group Number | III |