Agarose LE (Molecular Biology Grade)

Product Description

Agarose LE (Low Electroendosmosis) is Molecular Biology Grade and is specifically designed for the superior separation of nucleic acids, with maximally crisp band resolution, and is also ideal for cloning and cloning related experiments.

GoldBio Agarose LE is refined in an advanced process that excludes the use of organic solvents. The result is a cleaner end-product with significantly reduced environmental impact. The agarose can be used for analyses of nucleic acids from 50 bp to 25 kbp, protein electrophoresis and various blotting protocols.

The low EEO of the agarose promotes increased electrophoretic mobility, yielding improved resolution and shorter run times. This also allows macromolecules and larger particles (subcellular fragments, viruses, etc.) to migrate more freely through the gel matrix. The consistently low EEO reduces band distortion (caused by counterflow) that can result from the presence of excessive sulfate-rich negative ions.

Agarose is a natural product that forms an inert matrix used in electrophoresis, chromatography and other molecular biology and biochemistry techniques. Likewise, it is neutral and easily derivatizable, so it is easy to bind to its structure proteins like enzymes, antigens or antibodies. Toxicity absence makes working with agarose very convenient.

Technical Specifications
Agarose LE

Molecular Biology Grade

EEO (Electroendomosis): ≤0.12
Sulfate: ≤0.1%
Gel Strength (1%): ≥1200 g/cm 2
Gelling Temperature: 36±1.5°C
Melting Temperature: 88±1.5°C
DNase/RNase Activity: None Detected
DNA Resolution ≥1000 bp: Finely Resolved                                            
Gel Background: Very Low
DNA Binding: Very Low 

   
 

Product Specifications

Catalog ID A-201
CAS # 9012-36-6
Storage/Handling Store at room temperature.

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