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  • object(anvilstl\commercio\models\Category)#425 (15) { ["featured_image_urls"]=> array(3) { ["small"]=> string(74) "https://s3.amazonaws.com/commercio/goldbio-2018/collection/small/G-250.jpg" ["medium"]=> string(75) "https://s3.amazonaws.com/commercio/goldbio-2018/collection/medium/G-250.jpg" ["large"]=> string(74) "https://s3.amazonaws.com/commercio/goldbio-2018/collection/large/G-250.jpg" } ["featured_image"]=> array(3) { ["small"]=> string(74) "https://s3.amazonaws.com/commercio/goldbio-2018/collection/small/G-250.jpg" ["medium"]=> string(75) "https://s3.amazonaws.com/commercio/goldbio-2018/collection/medium/G-250.jpg" ["large"]=> string(74) "https://s3.amazonaws.com/commercio/goldbio-2018/collection/large/G-250.jpg" } ["banner_image_urls"]=> array(0) { } ["productCount"]=> NULL ["_attributes":"yii\db\BaseActiveRecord":private]=> array(23) { ["id"]=> int(395) ["root"]=> int(1) ["lft"]=> int(53) ["rgt"]=> int(98) ["level"]=> int(2) ["name"]=> string(14) "Agarose Resins" ["url_name"]=> string(14) "agarose-resins" ["title"]=> string(0) "" ["short_description"]=> string(35) "

    Hub Page for Agarose Resins

    " ["long_description"]=> string(0) "" ["hide"]=> int(0) ["third_party_id"]=> NULL ["featured_image_id"]=> int(53) ["banner_image_id"]=> int(0) ["meta_description"]=> string(0) "" ["meta_keywords"]=> string(0) "" ["root_sort"]=> int(1562682406) ["type"]=> string(7) "product" ["condition_type"]=> int(1) ["condition_match_type"]=> int(1) ["sort_type"]=> int(3) ["items_per_page"]=> int(25) ["color"]=> string(21) "rgba(160, 221, 94, 1)" } ["_oldAttributes":"yii\db\BaseActiveRecord":private]=> array(23) { ["id"]=> int(395) ["root"]=> int(1) ["lft"]=> int(53) ["rgt"]=> int(98) ["level"]=> int(2) ["name"]=> string(14) "Agarose Resins" ["url_name"]=> string(14) "agarose-resins" ["title"]=> string(0) "" ["short_description"]=> string(35) "

    Hub Page for Agarose Resins

    " ["long_description"]=> string(0) "" ["hide"]=> int(0) ["third_party_id"]=> NULL ["featured_image_id"]=> int(53) ["banner_image_id"]=> int(0) ["meta_description"]=> string(0) "" ["meta_keywords"]=> string(0) "" ["root_sort"]=> int(1562682406) ["type"]=> string(7) "product" ["condition_type"]=> int(1) ["condition_match_type"]=> int(1) ["sort_type"]=> int(3) ["items_per_page"]=> int(25) ["color"]=> string(21) "rgba(160, 221, 94, 1)" } ["_related":"yii\db\BaseActiveRecord":private]=> array(0) { } ["_relationsDependencies":"yii\db\BaseActiveRecord":private]=> array(0) { } ["_errors":"yii\base\Model":private]=> NULL ["_validators":"yii\base\Model":private]=> NULL ["_scenario":"yii\base\Model":private]=> string(7) "default" ["_events":"yii\base\Component":private]=> array(6) { ["beforeInsert"]=> array(1) { [0]=> array(2) { [0]=> array(2) { [0]=> object(creocoder\nestedsets\NestedSetsBehavior)#426 (8) { ["treeAttribute"]=> string(4) "root" ["leftAttribute"]=> string(3) "lft" 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NULL } } ["afterUpdate"]=> array(1) { [0]=> array(2) { [0]=> array(2) { [0]=> object(creocoder\nestedsets\NestedSetsBehavior)#426 (8) { ["treeAttribute"]=> string(4) "root" ["leftAttribute"]=> string(3) "lft" ["rightAttribute"]=> string(3) "rgt" ["depthAttribute"]=> string(5) "level" ["operation":protected]=> NULL ["node":protected]=> NULL ["owner"]=> *RECURSION* ["_attachedEvents":"yii\base\Behavior":private]=> array(6) { ["beforeInsert"]=> string(12) "beforeInsert" ["afterInsert"]=> string(11) "afterInsert" ["beforeUpdate"]=> string(12) "beforeUpdate" ["afterUpdate"]=> string(11) "afterUpdate" ["beforeDelete"]=> string(12) "beforeDelete" ["afterDelete"]=> string(11) "afterDelete" } } [1]=> string(11) "afterUpdate" } [1]=> NULL } } ["beforeDelete"]=> array(1) { [0]=> array(2) { [0]=> array(2) { [0]=> object(creocoder\nestedsets\NestedSetsBehavior)#426 (8) { ["treeAttribute"]=> string(4) "root" ["leftAttribute"]=> string(3) "lft" ["rightAttribute"]=> string(3) "rgt" 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"afterInsert" ["beforeUpdate"]=> string(12) "beforeUpdate" ["afterUpdate"]=> string(11) "afterUpdate" ["beforeDelete"]=> string(12) "beforeDelete" ["afterDelete"]=> string(11) "afterDelete" } } [1]=> string(11) "afterDelete" } [1]=> NULL } } } ["_eventWildcards":"yii\base\Component":private]=> array(0) { } ["_behaviors":"yii\base\Component":private]=> array(1) { ["tree"]=> object(creocoder\nestedsets\NestedSetsBehavior)#426 (8) { ["treeAttribute"]=> string(4) "root" ["leftAttribute"]=> string(3) "lft" ["rightAttribute"]=> string(3) "rgt" ["depthAttribute"]=> string(5) "level" ["operation":protected]=> NULL ["node":protected]=> NULL ["owner"]=> *RECURSION* ["_attachedEvents":"yii\base\Behavior":private]=> array(6) { ["beforeInsert"]=> string(12) "beforeInsert" ["afterInsert"]=> string(11) "afterInsert" ["beforeUpdate"]=> string(12) "beforeUpdate" ["afterUpdate"]=> string(11) "afterUpdate" ["beforeDelete"]=> string(12) "beforeDelete" ["afterDelete"]=> string(11) "afterDelete" } } } ["__fieldGroups":protected]=> array(9) { [0]=> array(4) { ["name"]=> string(14) "Layout section" ["type"]=> string(7) "default" ["expanded"]=> bool(true) ["fieldGroups"]=> array(1) { [0]=> array(7) { ["id"]=> int(6) ["name"]=> string(13) "Content Block" ["slug"]=> string(13) "content-block" ["description"]=> NULL ["show_in_picker"]=> int(1) ["deleted_on"]=> NULL ["fields"]=> array(2) { [0]=> array(21) { ["id"]=> int(41) ["parent_id"]=> int(0) ["field_group_id"]=> int(6) ["name"]=> string(5) "Title" ["slug"]=> string(5) "title" ["placeholder"]=> NULL ["instructions"]=> string(0) "" ["type"]=> string(9) "TextField" ["max_length"]=> NULL ["min_length"]=> NULL ["min"]=> NULL ["max"]=> NULL ["pattern"]=> NULL ["required"]=> int(0) ["field_options"]=> NULL ["auto_complete"]=> NULL ["is_search_aggregation"]=> int(0) ["test"]=> NULL ["position"]=> int(1) ["children"]=> array(0) { } ["fieldChoices"]=> array(0) { } } [1]=> array(22) { ["id"]=> int(42) ["parent_id"]=> int(0) ["field_group_id"]=> int(6) ["name"]=> string(7) "Content" ["slug"]=> string(7) "content" ["placeholder"]=> NULL ["instructions"]=> string(0) "" ["type"]=> string(13) "RichTextField" ["max_length"]=> NULL ["min_length"]=> NULL ["min"]=> NULL ["max"]=> NULL ["pattern"]=> NULL ["required"]=> int(0) ["field_options"]=> NULL ["auto_complete"]=> NULL ["is_search_aggregation"]=> int(0) ["test"]=> NULL ["position"]=> int(2) ["children"]=> array(0) { } ["fieldChoices"]=> array(0) { } ["value"]=> string(905) "

    Characterization of protein structure and function relies on affinity chromatography to purify target proteins for downstream analysis. At GoldBio, we offer different affinity resins and columns including aminoethyl and glyoxal agarose beads, Glutathione S-transferase (GST) and nitrilotriacetic acid (NTA) resins, antibody ̶ Protein A and Protein G ̶ and histidine-tagged protein purification resins, immobilized enzyme resins, and magnetic, crosslinked and plain agarose beads. Below, you will also find our tested and trusted buffers and washes as well as the necessary equipment to help you obtain optimal binding and elution and achieve a high protein yield in small batch or large scale assays. You can also access our extensive collection of resources, designed to simplify your protein isolation procedure, that consists of detailed protocols, video demos and troubleshooting guides.

    " } } } } } [1]=> array(4) { ["name"]=> string(14) "Layout section" ["type"]=> string(7) "default" ["expanded"]=> bool(true) ["fieldGroups"]=> array(1) { [0]=> array(7) { ["id"]=> int(1) ["name"]=> string(6) "Banner" ["slug"]=> string(6) "banner" ["description"]=> NULL ["show_in_picker"]=> int(1) ["deleted_on"]=> NULL ["fields"]=> array(4) { [0]=> array(22) { ["id"]=> int(28) ["parent_id"]=> int(0) ["field_group_id"]=> int(1) ["name"]=> string(5) "Title" ["slug"]=> string(5) "title" ["placeholder"]=> NULL ["instructions"]=> string(0) "" ["type"]=> string(9) "TextField" ["max_length"]=> NULL ["min_length"]=> NULL ["min"]=> NULL ["max"]=> NULL ["pattern"]=> NULL ["required"]=> int(0) ["field_options"]=> NULL ["auto_complete"]=> NULL ["is_search_aggregation"]=> int(0) ["test"]=> NULL ["position"]=> int(1) ["children"]=> array(0) { } ["fieldChoices"]=> array(0) { } ["value"]=> string(14) "Agarose Resins" } [1]=> array(21) { ["id"]=> int(29) ["parent_id"]=> int(0) ["field_group_id"]=> int(1) ["name"]=> string(11) "Description" ["slug"]=> string(11) "description" ["placeholder"]=> NULL ["instructions"]=> string(0) "" ["type"]=> string(9) "TextField" ["max_length"]=> NULL ["min_length"]=> NULL ["min"]=> NULL ["max"]=> NULL ["pattern"]=> NULL ["required"]=> int(0) ["field_options"]=> NULL ["auto_complete"]=> NULL ["is_search_aggregation"]=> int(0) ["test"]=> NULL ["position"]=> int(2) ["children"]=> array(0) { } ["fieldChoices"]=> array(0) { } } [2]=> array(21) { ["id"]=> int(30) ["parent_id"]=> int(0) ["field_group_id"]=> int(1) ["name"]=> string(3) "Url" ["slug"]=> string(3) "url" ["placeholder"]=> NULL ["instructions"]=> string(0) "" ["type"]=> string(8) "UrlField" ["max_length"]=> NULL ["min_length"]=> NULL ["min"]=> NULL ["max"]=> NULL ["pattern"]=> NULL ["required"]=> int(0) ["field_options"]=> NULL ["auto_complete"]=> NULL ["is_search_aggregation"]=> int(0) ["test"]=> NULL ["position"]=> int(3) ["children"]=> array(0) { } ["fieldChoices"]=> array(0) { } } [3]=> array(22) { ["id"]=> int(31) ["parent_id"]=> int(0) ["field_group_id"]=> int(1) ["name"]=> string(5) "Image" ["slug"]=> string(5) "image" ["placeholder"]=> NULL ["instructions"]=> string(0) "" ["type"]=> string(10) "ImageField" ["max_length"]=> NULL ["min_length"]=> NULL ["min"]=> NULL ["max"]=> NULL ["pattern"]=> NULL ["required"]=> int(0) ["field_options"]=> NULL ["auto_complete"]=> NULL ["is_search_aggregation"]=> int(0) ["test"]=> NULL ["position"]=> int(4) ["children"]=> array(0) { } ["fieldChoices"]=> array(0) { } ["value"]=> array(4) { ["small"]=> string(109) "https://s3.amazonaws.com/commercio/goldbio-2018/uploads/images/default/small/Banner-AgaroseResins Smaller.png" ["medium"]=> string(110) "https://s3.amazonaws.com/commercio/goldbio-2018/uploads/images/default/medium/Banner-AgaroseResins Smaller.png" ["large"]=> string(109) "https://s3.amazonaws.com/commercio/goldbio-2018/uploads/images/default/large/Banner-AgaroseResins Smaller.png" ["original"]=> string(112) "https://s3.amazonaws.com/commercio/goldbio-2018/uploads/images/default/original/Banner-AgaroseResins Smaller.png" } } } } } } [2]=> array(4) { ["name"]=> string(14) "Layout section" ["type"]=> string(7) "default" ["expanded"]=> bool(true) ["fieldGroups"]=> array(1) { [0]=> array(7) { ["id"]=> int(6) ["name"]=> string(13) "Content Block" ["slug"]=> string(13) "content-block" ["description"]=> NULL ["show_in_picker"]=> int(1) ["deleted_on"]=> NULL ["fields"]=> array(2) { [0]=> array(21) { ["id"]=> int(41) ["parent_id"]=> int(0) ["field_group_id"]=> int(6) ["name"]=> string(5) "Title" ["slug"]=> string(5) "title" ["placeholder"]=> NULL ["instructions"]=> string(0) "" ["type"]=> string(9) "TextField" ["max_length"]=> NULL ["min_length"]=> NULL ["min"]=> NULL ["max"]=> NULL ["pattern"]=> NULL ["required"]=> int(0) ["field_options"]=> NULL ["auto_complete"]=> NULL ["is_search_aggregation"]=> int(0) ["test"]=> NULL ["position"]=> int(1) ["children"]=> array(0) { } ["fieldChoices"]=> array(0) { } } [1]=> array(22) { ["id"]=> int(42) ["parent_id"]=> int(0) ["field_group_id"]=> int(6) ["name"]=> string(7) "Content" ["slug"]=> string(7) "content" ["placeholder"]=> NULL ["instructions"]=> string(0) "" ["type"]=> string(13) "RichTextField" ["max_length"]=> NULL ["min_length"]=> NULL ["min"]=> NULL ["max"]=> NULL ["pattern"]=> NULL ["required"]=> int(0) ["field_options"]=> NULL ["auto_complete"]=> NULL ["is_search_aggregation"]=> int(0) ["test"]=> NULL ["position"]=> int(2) ["children"]=> array(0) { } ["fieldChoices"]=> array(0) { } ["value"]=> string(3315) "

    Select Resin Products

    • Our aminoethyl and glyoxal agarose resins, offered in low and high density formats, are designed for optimal protein immobilization since they have high binding capacity, high specificity, fast conjugation and provide highly reproducible results.
    • We provide high-quality chromatography equipment including gravity columns, regular and spin-mini columns to use in fast protein liquid chromatography (FPLC) and medium pressure liquid chromatography (MPLC) as well as empty columns that can be customized for your specific protein purification assay.
    • Our products also support affinity chromatography-facilitated isolation of recombinant proteins. GST-tagged proteins can be isolated through our glutathione resins in a one-step purification method allowing for rapid recovery. Histidine-tagged protein purification is made easy through our thoroughly tested cobalt, nickel, copper and zinc immobilized metal affinity chromatography (IMAC) IDA and NTA resins.
    • We also offer nickel NTA magnetic agarose beads, which are a suitable and convenient alternative for small scale purification of his-tagged proteins in native or denaturing conditions. In addition, our ready-to-use washing and elution buffers, His-tag buffer set and imidazole are available to meet your purification needs.
    • Our immobilized enzyme resins feature immobilized ficin, papain and pepsin agarose resins that facilitate the generation of F(ab’)2 fragments and small Fc peptide fragments. Immobilized pepstatin and trypsin agarose resins allow enrichment of a variety of specific macromolecules.
    • We carry a large range of crosslinked and plain agarose beads for effective isolation of proteins through size exclusion chromatography.
    " } } } } } [3]=> array(4) { ["name"]=> string(14) "Layout section" ["type"]=> string(7) "default" ["expanded"]=> bool(true) ["fieldGroups"]=> array(1) { [0]=> array(7) { ["id"]=> int(12) ["name"]=> string(18) "Thumbnail Repeater" ["slug"]=> string(18) "thumbnail-repeater" ["description"]=> NULL ["show_in_picker"]=> int(1) ["deleted_on"]=> NULL ["fields"]=> array(1) { [0]=> array(22) { ["id"]=> int(60) ["parent_id"]=> int(0) ["field_group_id"]=> int(12) ["name"]=> string(18) "Thumbnail Repeater" ["slug"]=> string(18) "thumbnail-repeater" ["placeholder"]=> NULL ["instructions"]=> string(0) "" ["type"]=> string(13) "RepeaterField" ["max_length"]=> NULL ["min_length"]=> NULL ["min"]=> NULL ["max"]=> NULL ["pattern"]=> NULL ["required"]=> int(0) ["field_options"]=> array(3) { ["id"]=> int(4) ["name"]=> string(9) "Thumbnail" ["slug"]=> string(9) "thumbnail" } ["auto_complete"]=> NULL ["is_search_aggregation"]=> int(0) ["test"]=> NULL ["position"]=> int(1) ["children"]=> array(0) { } ["fieldChoices"]=> array(3) { ["id"]=> int(4) ["name"]=> string(9) "Thumbnail" ["slug"]=> string(9) "thumbnail" } ["value"]=> array(12) { [0]=> array(7) { ["id"]=> int(4) ["name"]=> string(9) "Thumbnail" ["slug"]=> string(9) "thumbnail" ["description"]=> NULL ["show_in_picker"]=> int(1) ["deleted_on"]=> NULL ["fields"]=> array(4) { [0]=> array(22) { ["id"]=> int(33) ["parent_id"]=> int(0) ["field_group_id"]=> int(4) ["name"]=> string(5) "Image" ["slug"]=> string(5) "image" ["placeholder"]=> NULL ["instructions"]=> string(0) "" ["type"]=> string(10) "ImageField" ["max_length"]=> NULL ["min_length"]=> NULL ["min"]=> NULL ["max"]=> NULL ["pattern"]=> NULL ["required"]=> int(0) ["field_options"]=> NULL ["auto_complete"]=> NULL ["is_search_aggregation"]=> int(0) ["test"]=> NULL ["position"]=> int(1) ["children"]=> array(0) { } ["fieldChoices"]=> array(0) { } ["value"]=> array(4) { ["small"]=> string(93) 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    Discover Protein Purification Resources

    Here you will find charts, guides, tools, video demonstrations, detailed protocols and in-depth articles that will aid in planning the purification workflow of your target protein depending on the downstream application. You will also find information to help you compare binding characteristics of various resins and different GoldBio products. We have also developed comprehensive troubleshooting articles that contain solutions to the most common affinity chromatography problems.

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"https://www.goldbio.com/documents/1414/Enzyme%20and%20Antibody%20Immobilization%20Protocol%20utilizing%20Glyoxal%20Agarose%20Beads.pdf" } [3]=> array(22) { ["id"]=> int(52) ["parent_id"]=> int(0) ["field_group_id"]=> int(8) ["name"]=> string(10) "Icon Image" ["slug"]=> string(5) "image" ["placeholder"]=> NULL ["instructions"]=> string(42) "Use square images (75x75) for best results" ["type"]=> string(10) "ImageField" ["max_length"]=> NULL ["min_length"]=> NULL ["min"]=> NULL ["max"]=> NULL ["pattern"]=> NULL ["required"]=> int(0) ["field_options"]=> NULL ["auto_complete"]=> NULL ["is_search_aggregation"]=> int(0) ["test"]=> NULL ["position"]=> int(4) ["children"]=> array(0) { } ["fieldChoices"]=> array(0) { } ["value"]=> array(4) { ["small"]=> string(103) "https://s3.amazonaws.com/commercio/goldbio-2018/uploads/images/default/small/Resins Column Chrom -8.png" ["medium"]=> string(104) "https://s3.amazonaws.com/commercio/goldbio-2018/uploads/images/default/medium/Resins Column Chrom -8.png" ["large"]=> string(103) "https://s3.amazonaws.com/commercio/goldbio-2018/uploads/images/default/large/Resins Column Chrom -8.png" ["original"]=> string(106) "https://s3.amazonaws.com/commercio/goldbio-2018/uploads/images/default/original/Resins Column Chrom -8.png" } } } } } } } } } } [8]=> array(4) { ["name"]=> string(14) "Layout section" ["type"]=> string(7) "default" ["expanded"]=> bool(true) ["fieldGroups"]=> array(1) { [0]=> array(7) { ["id"]=> int(1) ["name"]=> string(6) "Banner" ["slug"]=> string(6) "banner" ["description"]=> NULL ["show_in_picker"]=> int(1) ["deleted_on"]=> NULL ["fields"]=> array(4) { [0]=> array(22) { ["id"]=> int(28) ["parent_id"]=> int(0) ["field_group_id"]=> int(1) ["name"]=> string(5) "Title" ["slug"]=> string(5) "title" ["placeholder"]=> NULL ["instructions"]=> string(0) "" ["type"]=> string(9) "TextField" ["max_length"]=> NULL ["min_length"]=> NULL ["min"]=> NULL ["max"]=> NULL ["pattern"]=> NULL ["required"]=> int(0) ["field_options"]=> NULL ["auto_complete"]=> NULL ["is_search_aggregation"]=> int(0) ["test"]=> NULL ["position"]=> int(1) ["children"]=> array(0) { } ["fieldChoices"]=> array(0) { } ["value"]=> string(16) "Reducing Agents " } [1]=> array(22) { ["id"]=> int(29) ["parent_id"]=> int(0) ["field_group_id"]=> int(1) ["name"]=> string(11) "Description" ["slug"]=> string(11) "description" ["placeholder"]=> NULL ["instructions"]=> string(0) "" ["type"]=> string(9) "TextField" ["max_length"]=> NULL ["min_length"]=> NULL ["min"]=> NULL ["max"]=> NULL ["pattern"]=> NULL ["required"]=> int(0) ["field_options"]=> NULL ["auto_complete"]=> NULL ["is_search_aggregation"]=> int(0) ["test"]=> NULL ["position"]=> int(2) ["children"]=> array(0) { } ["fieldChoices"]=> array(0) { } ["value"]=> string(315) "At the protein level, reducing agents are often critical in cleaving the disulfide bonds between cysteine amino acids. Here, we discuss the function of DTT (dithiothreitol), DTE (dithioerythritol), L-glutathione (GSH) and TCEP (Tris (2-Carboxyethyl) phosphine hydrochloride) and their role in protein purification. " } [2]=> array(22) { ["id"]=> int(30) ["parent_id"]=> int(0) ["field_group_id"]=> int(1) ["name"]=> string(3) "Url" ["slug"]=> string(3) "url" ["placeholder"]=> NULL ["instructions"]=> string(0) "" ["type"]=> string(8) "UrlField" ["max_length"]=> NULL ["min_length"]=> NULL ["min"]=> NULL ["max"]=> NULL ["pattern"]=> NULL ["required"]=> int(0) ["field_options"]=> NULL ["auto_complete"]=> NULL ["is_search_aggregation"]=> int(0) ["test"]=> NULL ["position"]=> int(3) ["children"]=> array(0) { } ["fieldChoices"]=> array(0) { } ["value"]=> string(60) "https://www.goldbio.com/search?q=redagentsarticle1&type=blog" } [3]=> array(22) { ["id"]=> int(31) ["parent_id"]=> int(0) ["field_group_id"]=> int(1) ["name"]=> string(5) "Image" ["slug"]=> string(5) "image" ["placeholder"]=> NULL ["instructions"]=> string(0) "" ["type"]=> string(10) "ImageField" ["max_length"]=> NULL ["min_length"]=> NULL ["min"]=> NULL ["max"]=> NULL ["pattern"]=> NULL ["required"]=> int(0) ["field_options"]=> NULL ["auto_complete"]=> NULL ["is_search_aggregation"]=> int(0) ["test"]=> NULL ["position"]=> int(4) ["children"]=> array(0) { } ["fieldChoices"]=> array(0) { } ["value"]=> array(4) { ["small"]=> string(105) "https://s3.amazonaws.com/commercio/goldbio-2018/uploads/images/default/small/Bottombanner C - smaller.png" ["medium"]=> string(106) "https://s3.amazonaws.com/commercio/goldbio-2018/uploads/images/default/medium/Bottombanner C - smaller.png" ["large"]=> string(105) "https://s3.amazonaws.com/commercio/goldbio-2018/uploads/images/default/large/Bottombanner C - smaller.png" ["original"]=> string(108) "https://s3.amazonaws.com/commercio/goldbio-2018/uploads/images/default/original/Bottombanner C - smaller.png" } } } } } } } }

    • Highest Density Nickel

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    Product Description

    GoldBio’s new Highest Density Nickel Agarose is a high capacity resin manufactured with a polychelator ligand. The product was developed to work in the presence of EDTA and DTT without any loss of performance. Its specificity and stability allows a one-step purification, eliminating the need for pretreatment of samples that cause nickel stripping.

    Our Highest Density Nickel Agarose is supplied as a suspension in 50% aqueous suspension containing 20% ethanol. Purifications can be performed using 20mM EDTA and 20mM DTT and can work in both native as well as denaturing conditions.

    Product Specifications
    Bead Geometry/Size: Spherical - ~50-150 µm

    Crosslinking: Highly Crosslinked

    Agarose: 6%

    Chemical Stability:

    • 20mM DTT
    • 20mM EDTA
    • 8M urea
    • 6M guanidine hydrochloride
    • 30% acetonitrile
    • 100% methanol
    • 100% ethanol
    • Buffer solutions at pH 4 - 9

    Loading Capacity: >60 μmol me2+/ml gel

    Static Binding Capacity: ≥80 mg/ml gel

    Antimicrobial Agent: 20% ethanol

    Storage/Handling: Store at 4°C. Do NOT freeze.

    Technical Documentation

    P Protocol
    O Other
    C Certificate of Analysis
    S Safety Data Sheet
    P
    Affinity His-Tag Purification Troubleshooting

    Troubleshooting help for His-Tag Protein Purification
    P
    Affinity vs. Specificity

    Metal Ion Affinity vs. Specificity Graph for His-Tag Protein Purification
    P
    Affinity His-Tag Purification Utilizing Chelating Agarose Beads

    Detailed Instructions for Agarose Bead Use, Column Packing and Regeneration
    P
    GoldBio His-Tag Column Prep Protocol

    Quick and Simple His-Tag Column Prep protocol including Pouring and Packing an Agarose Column
    P
    Affinity His-Tag Purification utilizing Chelating Agarose Bead Spin Columns

    Protocol for the use of His-Tag purification with GoldBio Spin Columns

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    H-320-5 5 mL $ 53.00
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    Nickel Agarose Beads (Low Density)
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    P-307-25 1 x 25 each $ 128.00
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