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Competent Cells and Transformation
GoldBio offers an extensive collection of efficient chemically competent and electrocompetent cells with high transformation efficiencies to match your specific downstream applications: general cloning, protein expression, library construction, and many more.
- GoldBio's wide range of chemically competent and electrocompetent cells are optimized to provide lot-to-lot consistency and up to 10X higher transformation efficiency.
- Our universal recovery medium (SOC) formulation accompanies ALL of GoldBio's competent cells.
- We carry multiple Endonuclease I-deficient (endA1) competent bacterial cell strains to suit your cloning needs and ensure your transformations result in high quality and yield of plasmid DNA.
- Choose from E. coli strains with different selectable markers and screening options including the classic blue/white selection.
- GoldBio cells are ideal for distinct downstream applications from routine cloning/subcloning and protein expression to the more complex library construction, and cloning of difficult targets.
- Our electrocompetent Agrobacterium cells contain an efficient T-DNA binary system for genetic material transfer into the host plant cells.
CHOOSE THE BEST COMPETENT CELLS FOR YOUR APPLICATION!
Our GB5-alpha™, BL21, BL21(DE3), GB10B™, GB10B-Pro™, DL39(DE3), and Agrobacterium cells are available in multiple formats to provide you with flexibility when planning your experiments. GoldBio also provides easy-to-follow protocols, FAQs, protocol videos and selection guides to ensure success of your transformations!
Use GoldBio's Competent Cell Guide to Select the Right Competent Cells for your Assay
Includes transformation efficiencies, genotype, strain information, screening methods and applicatio...
Read more!
BROWSE OUR TRANSFORMATION AND COMPETENT CELL RESOURCES
Your time in the lab is valuable! In this section you will find GoldBio's transformation tools designed to help support and simplify your competent cell transformations. You can access specific chemical transformation or electroporation protocols for each of our competent cell lines, antibiotic information, colony screening protocols, instructional blog articles, detailed guides containing competent cells characteristics and downstream applications, FAQs, electroporation troubleshooting tips and video protocols.
Electroporation and Electrocompetent Cells FAQs:
What is electroporation?
Electroporation is a technique commonly used in transformation assays to introduce DNA into an electrocompetent cell. During electroporation a transient current or electrical pulse is applied to cells that have been suspended in a solution. The current momentarily increases the permeability of the cell's membrane, creating holes (a pathway) that allow DNA (plasmids) to pass through and enter the cell. Generally, electroporation can be more effective than chemical transformation.
When should I use electrocompetent cells?
Electrocompetent cells have a higher transformation efficiency than chemically competent cells. The use of electrocompetent cells facilitate the transformation of large plasmids, including BACs (Bacterial Artificial Chromosomes), that might otherwise be difficult to transform using chemical transformation.
How do I prevent arcing during electroporation?
Arcing during electroporation can be avoided by making sure there are no air bubbles in the cell/DNA solution prior to electroporation. Also, desalt your DNA since the presence of conductive ions in the solution will cause arcing and electroporation failure. In addition, ensure that your equipment, including the cuvette is properly cleaned and in good condition.
Showing 41-43 of 43 items.
Puc19 Plasmid
| Catalog ID | Size | Pricing | |
|---|---|---|---|
| P-550-10 | 10 μg | $ 25.00 |
Description
pUC19 Vector Plasmid is a compact, high-copy cloning vector with Ampicillin resistance and a versatile tool in molecular biology research. pUC19 has 2686 base pairs which harbor a 54 base-pair multiple cloning site (MCS) for efficient cloning with 13 unique restriction enzyme sites, offering flexibility and compatibility with a variety of experimental setups. It is identical to pUC18, except that their MCS are oriented opposite of each other.
RR1 Chemically Competent E. coli Cells
| Catalog ID | Size | Pricing | |
|---|---|---|---|
| CC-113-5x50 | 5 x 50 µl |
|
$ 48.00 |
| CC-113-10x50 | 10 x 50 µl |
|
$ 84.00 |
Description
GoldBio’s RR1 chemically competent E. coli cells are suitable for high-efficiency transformation in a wide variety of applications such as cloning and sub-cloning. E. coli RR1 is a recA+ derivative of the HB101 strain and can be more useful than HB101 if a recA+ background is required.
Product Specifications
Competent cell type: Chemically competent
Derivative of: RR1
Species: E. coli
Format: Tubes
Transformation efficiency: ≥3 x 107 cfu/µg pUC19 DNA
Blue/white screening: No
Product Specifications
| Catalog ID | CC-113 |
|---|---|
| Storage/Handling | Store Competent Cells at -80°C. |
TG1 Phage Display Electrocompetent Cells
| Catalog ID | Size | Pricing | |
|---|---|---|---|
| CC-205-5x100 | 5 x 100 μL |
|
$ 151.00 |
| CC-205-10x100 | 10 x 100 μL |
|
$ 261.00 |
| CC-205-15x100 | 15 x 100 μL |
|
$ 381.00 |
Description
GoldBio’s TG1 Phage Display Electrocompetent E. coli cells are suitable for protein expression and preparation of antibody or peptide phage display libraries.TG1 Phage Display cells have ≥1 x 109 cfu/µg efficiency with electroporation and have Amber suppressor strain (supE).
Product Specifications
Competent cell type: ElectroCompetent
Species: E. coli
Format: Tubes
Transformation efficiency: ≥1 x 109 cfu/µg pUC19 DNA
Blue/white screening: Yes
