Enzymes

Enzymes assist in carrying out crucial cellular functions, and GoldBio provides a variety of enzymes to accommodate your research needs, including alkaline phosphatase, DNase, RNase, lysozyme, horseradish peroxidase, pepsin and trypsin among others. These products are offered at a lower rate than other commercially available enzymes without sacrificing quality so that researchers attain the highest value possible.
Showing 1-20 of 41 items.

Alkaline Phosphatase, 2-Component System

There is no image for Alkaline Phosphatase, 2-Component System Alkaline Phosphatase, 2-Component System
Catalog ID Size Pricing
A-440-1 1 KU $ 65.00

Description

Alkaline phosphatase is an enzyme commonly used for the removal of terminal phosphates from the 5' end of DNA or RNA strands to prevent reannealing of the cohesive ends and facilitate future radiolabeling of the strands.

This system includes 30 U/μL of alkaline phosphatase in a 50% glycerol solution and a dilution buffer that allows for the preparation of a 3 U/μL solution for use in DNA modification.

Product Specifications

Catalog ID A-440
Grade BIOTECHNOLOGY GRADE
Storage/Handling store at 4°C. Do NOT freeze.
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A-440-1 1 KU $ 65.00

AquaSpark™ Alkaline Phosphatase Substrate, 2mM in DMSO, Patent pending

Catalog ID Size Pricing
AQ-100-100 100 μL $ 117.00

Description

AquaSpark™ Alkaline Phosphatase Substrate is a chemiluminescent substrate for alkaline phosphatase (AP) detection in immunoassays, in particular in ELISA. AquaSpark™ Alkaline Phosphatase Substrate is also a suitable substrate for protein (Western) blotting or nucleic acid blotting procedures using AP-conjugated antibodies. AquaSpark™ probes offer unique advantages as they can work as single agents, they have a higher efficiency and sensitivity over currently existing probes.

Product Specifications

Name AquaSpark™ Alkaline Phosphatase Substrate, 2mM in DMSO
Formula C21H24NaO9P
MW 474.37 g/mol
Storage/Handling Store at 4°C. Protect from light.
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AQ-100-100 100 μL $ 117.00

AquaSpark™ Broad Range Phosphatase Substrate, 2mM in DMSO, Patent pending

Catalog ID Size Pricing
AQ-105-100 100 μL $ 117.00

Description

AquaSpark™ Broad Range Phosphatase Substrate is superior to other luminogenic alkaline phosphatase substrates as it shows significantly higher signal intensity, working as a single reagent without the need for enhancers. AquaSpark™ Broad Range Phosphatase Substrate works under various pH conditions, from near-neutral to alkaline and in contrast to conventionally used dioxetanes, AquaSpark™ Broad Range Phosphatase Substrate is water-soluble.

AquaSpark™ Broad Range Phosphatase Substrate can be used to detect free phosphatase activity in aqueous solutions and under physiological conditions and is suitable for immunoassay applications, such as Western blotting and nucleic acid blotting procedures (Southern and Northern blotting) using AP-conjugated detection molecules. Strong light emission and very low background make AquaSpark™ Broad Range Phosphatase Substrate the superior substrate for ELISA applications in research or diagnostics.

Product Specifications

Name AquaSpark Broad Range Phosphatase Substrate, 2mM in DMSO
Formula C28H30ClOP
MW 592.96 g/mol
Storage/Handling Store at 4C. Protect from light.
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AQ-105-100 100 μL $ 117.00

Cas9 Nuclease (10µM)

There is no image for Cas9 Nuclease (10µM) Cas9 Nuclease (10µM)
Catalog ID Size Pricing
C-529-80 80 µg (500 pmol) $ 155.00
C-529-400 400 µg (2,500 pmol) $ 510.00

Description

Cas9 Nuclease is the purified recombinant Streptococcus pyogenes Cas9 enzyme containing a nuclear localization signal (NLS) at the C-terminal for targeting to the nucleus. This enzyme is designed to perform CRISPR/Cas9-mediated genome editing. The physical purity of this enzyme is ≥98% as assessed by SDS-PAGE with Coomassie blue staining.

CRISPR (Clustered regularly interspaced short palindromic repeats)/CRISPR-associated (Cas) systems provide bacteria and archaea with adaptive immunity against viruses and plasmids by using CRISPR RNAs (crRNAs) to guide the silencing of invading nucleic acids. The CRISPR system consists of a short non-coding guide RNA (sgRNA) made up of a target complementary CRISPR RNA (crRNA) and an auxiliary transactivating crRNA (tracrRNA). The sgRNA guides the Cas9 endonuclease to a specific genomic locus via base pairing between the crRNA sequence and the target sequence, and cleaves the DNA to create a double-strand break. The location of the break is within the target sequence 3 bases from the NGG PAM (Protospacer Adjacent Motif). The PAM sequence, NGG, must follow the targeted region on the opposite strand of the DNA with respect to the region complementary sgRNA sequence.

Cas9 nuclease is free from detectable RNase, Endonuclease (nicking) and non-specific DNase activities.

Product Includes

  • Cas9 Nuclease (1600 ng/µl (10µM))
  • 10x Cas9 Nuclease Reaction Buffer

Source: E. coli BL21 (DE3) strain expressing a Cas9 gene from Streptococcus pyogenes with an N-terminal 6xHis tag and C-terminal SV40 nuclear localization signal (NLS)

Product Specifications

Catalog ID C-529
Storage/Handling Store at -20°C.
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C-529-80 80 µg (500 pmol) $ 155.00
C-529-400 400 µg (2,500 pmol) $ 510.00

Cas9 Nuclease (1µM)

There is no image for Cas9 Nuclease (1µM) Cas9 Nuclease (1µM)
Catalog ID Size Pricing
C-519-40 40 µg (250 pmol) $ 93.00

Description

Cas9 Nuclease is the purified recombinant Streptococcus pyogenes Cas9 enzyme containing a nuclear localization signal (NLS) at the C-terminal for targeting to the nucleus. This enzyme is designed to perform CRISPR/Cas9-mediated genome editing. The physical purity of this enzyme is ≥98% as assessed by SDS-PAGE with Coomassie blue staining.

CRISPR (Clustered regularly interspaced short palindromic repeats)/CRISPR-associated (Cas) systems provide bacteria and archaea with adaptive immunity against viruses and plasmids by using CRISPR RNAs (crRNAs) to guide the silencing of invading nucleic acids. The CRISPR system consists of a short non-coding guide RNA (sgRNA) made up of a target complementary CRISPR RNA (crRNA) and an auxiliary transactivating crRNA (tracrRNA). The sgRNA guides the Cas9 endonuclease to a specific genomic locus via base pairing between the crRNA sequence and the target sequence, and cleaves the DNA to create a double-strand break. The location of the break is within the target sequence 3 bases from the NGG PAM (Protospacer Adjacent Motif). The PAM sequence, NGG, must follow the targeted region on the opposite strand of the DNA with respect to the region complementary sgRNA sequence.

Cas9 nuclease is free from detectable RNase, Endonuclease (nicking) and non-specific DNase activities.

Product Specifications

Catalog ID C-519
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C-519-40 40 µg (250 pmol) $ 93.00

Cellulase R-10

There is no image for Cellulase R-10 Cellulase R-10
Catalog ID Size Pricing
C8001.0001 1 g $ 83.00
C8001.0005 5 g $ 162.00
C8001.0010 10 g $ 253.00

Description

Cellulase R-10, also called Cellulase "Onozuka" R-10, is derived from Trichoderma Viride. Cellulase R-10 is a multi-enzymatic system consisting of various enzymes, including cellulase, hemicellulase, pectinase and protease. Cellulase R-10 is typically used in association with macerozyme R-10 in the decomposition of plant cell walls to produce protoplasts.

Product Specifications

Catalog ID C8001
CAS # 9012-54-8
Storage/Handling Store at room temperature.
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C8001.0001 1 g $ 83.00
C8001.0005 5 g $ 162.00
C8001.0010 10 g $ 253.00

Cellulase RS

There is no image for Cellulase RS Cellulase RS
Catalog ID Size Pricing
C8003.0001 1 g $ 128.00
C8003.0005 5 g $ 351.00
C8003.0010 10 g $ 552.00

Description

Cellulase “Onozuka RS” is produced by a mutant Trichoderma viride that was derived from the parent strain for Cellulase “Onozuka -R-10”. Cellulase RS contains a very high activity of decomposing natural celluloses. This type of cellulase can be used to obtain protoplasts in a very short time and dissolves cell walls of a wider range of plants.

Product Specifications

Catalog ID C8003
CAS # 9012-54-8
Storage/Handling Store desiccated at 4°C.
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C8003.0001 1 g $ 128.00
C8003.0005 5 g $ 351.00
C8003.0010 10 g $ 552.00

Coenzyme II (β-NADPH)

Catalog ID Size Pricing
B-625-5 5 g $ 179.00
B-625-10 10 g $ 275.00
B-625-25 25 g $ 598.00
B-625-50 50 g $ 1,041.00

Description

Known in its anhydrous form as Coenzyme II, this substance is a fundamental metabolite studied in nucleotide chemistry. Nicotinamide dinucleotide phosphate (NADP) is a coenzyme carrying electrons and hydrogen in a multitude of reactions. Forms include oxidized NADP+ and reduced NADPH, a product of the pentose phosphate pathway (PPP). The molecule acts as an electron acceptor and the conjugate acid to NADP zwitterion.

NADP functions in electron transfer in the anabolic biosynthesis of lipids, cholesterol and fatty acyl chains. The substance can therefore be applied to antioxidant roles preventing oxidative damage. NADP+ and NADPH, paired together for redox reactions, participate in enzyme oxidation-reduction catalysis. NADP is further implicated in metabolism, mitochondrial conditions and apoptosis-induction.

Product Specifications

Catalog ID B-625
CAS # 53-59-8
MW 743.40 g/mol
Storage/Handling Store desiccated at 4°C. Protect from light and air.
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B-625-5 5 g $ 179.00
B-625-10 10 g $ 275.00
B-625-25 25 g $ 598.00
B-625-50 50 g $ 1,041.00

DNase I, Bovine Pancreas (Lysate Tested), >2000 Kunitz U/mg

There is no image for DNase I, Bovine Pancreas (Lysate Tested), >2000 Kunitz U/mg DNase I, Bovine Pancreas (Lysate Tested), >2000 Kunitz U/mg
Catalog ID Size Pricing
D-301-50 50 mg $ 69.00
D-301-100 100 mg $ 101.00
D-301-500 500 mg $ 419.00
D-301-1 1 g $ 799.00

Description

DNase I (Deoxyribonuclease I) is a dialyzed lyophilized powder from bovine pancreas that has been purified by chromatography.

Bovine pancreatic deoxyribonuclease (DNase) is an endonuclease, which splits phosphodiester linkages preferentially adjacent to a pyrimidine nucleotide yielding polynucleotides with a free hydroxyl group at the 3' position and a phosphate group at the 5' position. The optimum pH is 7.8.

DNase I is activated by bivalent metals and inhibited by chelating agents such as EDTA and SDS. DNase I can be stabilized against proteolytic digestion by 5mM Ca2+.

Product Specifications

Catalog ID D-301
CAS # 9003-98-9
Grade MOLECULAR BIOLOGY GRADE
Storage/Handling Store desiccated at -20°C.
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D-301-50 50 mg $ 69.00
D-301-100 100 mg $ 101.00
D-301-500 500 mg $ 419.00
D-301-1 1 g $ 799.00

DNase I, Bovine Pancreas (Lysate Tested), >300 Kunitz U/mg

There is no image for DNase I, Bovine Pancreas (Lysate Tested), >300 Kunitz U/mg DNase I, Bovine Pancreas (Lysate Tested), >300 Kunitz U/mg
Catalog ID Size Pricing
D-303-100 100 mg $ 30.00
D-303-500 500 mg $ 89.00
D-303-1 1 g $ 161.00
D-303-5 5 g $ 657.00

Description

DNase I (Deoxyribonuclease I) is a dialyzed lyophilized powder from bovine pancreas that has been purified by chromatography.

Bovine pancreatic deoxyribonuclease (DNase) is an endonuclease which splits phosphodiester linkages, preferentially adjacent to a pyrimidine nucleotide yielding polynucleotides with a free hydroxyl group at the 3' position and phosphate group at the 5' position. The optimum pH is 7.8.

DNase I is activated by bivalent metals, and inhibited by chelating agents such as EDTA and SDS. DNase I can be stabilized against proteolytic digestion by 5mM Ca 2+.

Product Specifications

Name DNase I
CAS # 9003-98-9
Grade MOLECULAR BIOLOGY GRADE
Storage/Handling Store desiccated at -20°C.
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D-303-100 100 mg $ 30.00
D-303-500 500 mg $ 89.00
D-303-1 1 g $ 161.00
D-303-5 5 g $ 657.00

DNase I, Bovine Pancreas (Lysate Tested), >500 Kunitz U/mg

There is no image for DNase I, Bovine Pancreas (Lysate Tested), >500 Kunitz U/mg DNase I, Bovine Pancreas (Lysate Tested), >500 Kunitz U/mg
Catalog ID Size Pricing
D-300-100 100 mg $ 37.00
D-300-500 500 mg $ 111.00
D-300-1 1 g $ 201.00
D-300-5 5 g $ 822.00

Description

DNase I (Deoxyribonuclease I) is a dialyzed lyophilized powder from bovine pancreas that has been purified by chromatography.

Bovine pancreatic deoxyribonuclease (DNase) is an endonuclease which splits phosphodiester linkages, preferentially adjacent to a pyrimidine nucleotide yielding polynucleotides with a free hydroxyl group at the 3' position and phosphate group at the 5' position. The optimum pH is 7.8.

DNase I is activated by bivalent metals, and inhibited by chelating agents such as EDTA and SDS. DNase I can be stabilized against proteolytic digestion by 5 mM Ca2+.

Product Specifications

Catalog ID D-300
CAS # 9003-98-9
Grade MOLECULAR BIOLOGY GRADE
Storage/Handling Store desiccated at -20°C.
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D-300-100 100 mg $ 37.00
D-300-500 500 mg $ 111.00
D-300-1 1 g $ 201.00
D-300-5 5 g $ 822.00

Elastase, Porcine Pancreas

There is no image for Elastase, Porcine Pancreas Elastase, Porcine Pancreas
Catalog ID Size Pricing
E-240-100 100 mg $ 45.00
E-240-500 500 mg $ 75.00
E-240-1 1 g $ 135.00
E-240-5 5 g $ 360.00

Description

Elastase is used for tissue dissociation and cell isolation applications. Elastase has an activity of >30 units/mg and is unstable at < pH 3.5. Elastase was purified from a porcine pancreas and then purified by affinity chromatography to remove trace amounts of contaminating proteases. One unit corresponds to the amount of enzyme which liberates 1 µmol 4-nitroaniline per minute at 25°C and pH 7.8.

Product Specifications

Catalog ID E-240
CAS # 39445-21-1
Grade MOLECULAR BIOLOGY GRADE
Storage/Handling Store at -20°C.
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E-240-100 100 mg $ 45.00
E-240-500 500 mg $ 75.00
E-240-1 1 g $ 135.00
E-240-5 5 g $ 360.00

Glucose Oxidase >100 U/mg

There is no image for Glucose Oxidase >100 U/mg Glucose Oxidase >100 U/mg
Catalog ID Size Pricing
G-040-50 50 KU $ 35.00
G-040-250 250 KU $ 135.00
G-040-1 1 MU $ 415.00

Description

Glucose oxidase comes from Aspergillus niger and has many applications. Glucose oxidase can be used in the determination of glucose in colorimetric assays in conjunction with peroxidase, in the determination of glucose biosensors or for use as an enzyme label.

Product Specifications

Catalog ID G-040
Grade MOLECULAR BIOLOGY GRADE
Storage/Handling Store at -20°C.
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G-040-50 50 KU $ 35.00
G-040-250 250 KU $ 135.00
G-040-1 1 MU $ 415.00

Glucose Oxidase >225 U/mg

There is no image for Glucose Oxidase >225 U/mg Glucose Oxidase >225 U/mg
Catalog ID Size Pricing
G-041-50 50 KU $ 35.00
G-041-250 250 KU $ 135.00
G-041-1 1 MU $ 415.00

Description

Glucose oxidase comes from Aspergillus niger and has many applications. Glucose oxidase can be used in the determination of glucose in colorimetric assays in conjunction with peroxidase, in the determination of glucose biosensors or for use as en enzyme label.

Product Specifications

Catalog ID G-041
Grade MOLECULAR BIOLOGY GRADE
Storage/Handling Store at -20°C.
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G-041-50 50 KU $ 35.00
G-041-250 250 KU $ 135.00
G-041-1 1 MU $ 415.00

Horseradish peroxidase (HRP)

Catalog ID Size Pricing
P-100-100 100 mg $ 65.00
P-100-250 250 mg $ 121.00
P-100-500 500 mg $ 200.00
P-100-1 1 g $ 344.00

Description

Horseradish peroxidase (HRP) is the most commonly used antibody-conjugated enzyme. Substrates for HRP are luminol and benzidine substrates. Tetramethylbenzidine (TMB) and similar substrates are oxidized by HRP in the presence of hydrogen peroxide, or nontoxic substitutes, to yield a chinoid structure of intense blue color.

Product Specifications

Catalog ID P-100
CAS # 9003-99-0
MW ~40,000 g/mol
Grade MOLECULAR BIOLOGY GRADE
Storage/Handling Store at -20°C.
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P-100-100 100 mg $ 65.00
P-100-250 250 mg $ 121.00
P-100-500 500 mg $ 200.00
P-100-1 1 g $ 344.00

Hot Start Pfu DNA Polymerase

There is no image for Hot Start Pfu DNA Polymerase Hot Start Pfu DNA Polymerase
Catalog ID Size Pricing
P-650-200 200 Units $ 134.00
P-650-1000 1000 Units $ 608.00

Description

Hot Start Pfu DNA Polymerase is formulated with chemical modification which effectively neutralize 5´→ 3´ DNA polymerase and 3´→ 5´ exonuclease (proofreading) activities at room temperature, but regain the full enzyme activity upon the initial denaturation step. Hot start Pfu DNA polymerase retains the high fidelity, sensitivity and processivity of Pfu DNA polymerase, while provides reduced background by facilitating room temperature PCR assembly and preventing priming until stringent primer annealing temperatures are reached. Pfu DNA polymerase has an error rate six-fold lower than Taq DNA polymerase, and significantly lower than the error rates of most other proofreading enzymes or DNA polymerase mixtures. This product is supplied with a 10x PCR reaction buffer that provides a final concentration of 1.5mM Mg2+ and an optional 5X GC Enhancer that increases the amplification of GC rich templates up to 84%.

Product Specifications

Catalog ID P-650
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P-650-200 200 Units $ 134.00
P-650-1000 1000 Units $ 608.00

Hot Start Pfu DNA Polymerase plus dNTP

There is no image for Hot Start Pfu DNA Polymerase plus dNTP Hot Start Pfu DNA Polymerase plus dNTP
Catalog ID Size Pricing
P-655-200 200 Units $ 148.00
P-655-1000 1000 Units $ 659.00

Description

Hot Start Pfu DNA Polymerase is formulated with chemical modification which effectively neutralize 5´→ 3´ DNA polymerase and 3´→ 5´ exonuclease (proofreading) activities at room temperature, but regain the full enzyme activity upon the initial denaturation step. Hot start Pfu DNA polymerase retains the high fidelity, sensitivity and processivity of Pfu DNA polymerase, while provides reduced background by facilitating room temperature PCR assembly and preventing priming until stringent primer annealing temperatures are reached. Pfu DNA polymerase has an error rate six-fold lower than Taq DNA polymerase, and significantly lower than the error rates of most other proofreading enzymes or DNA polymerase mixtures. This product is supplied with a 10x PCR reaction buffer that provides a final concentration of 1.5mM Mg2+ and an optional 5X GC Enhancer that increases the amplification of GC rich templates up to 84%.

Product Specifications

Catalog ID P-655
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P-655-200 200 Units $ 148.00
P-655-1000 1000 Units $ 659.00

Hot Start Taq DNA Polymerase

There is no image for Hot Start Taq DNA Polymerase Hot Start Taq DNA Polymerase
Catalog ID Size Pricing
T-510-200 200 Units $ 62.00
T-510-1000 1000 Units $ 258.00

Description

Hot Start Taq DNA Polymerase is a chemically modified enzyme that remains inactive at room temperature and requires an increase in temperature for activation.

Product Specifications

Catalog ID T-510
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T-510-200 200 Units $ 62.00
T-510-1000 1000 Units $ 258.00

Hot Start Taq DNA Polymerase plus dNTP

There is no image for Hot Start Taq DNA Polymerase plus dNTP Hot Start Taq DNA Polymerase plus dNTP
Catalog ID Size Pricing
T-511-200 200 Units $ 76.00
T-511-1000 1000 Units $ 304.00

Description

Hot start Taq is a thermostable DNA polymerase that possesses a 5´→3´ polymerase activity and a 5´ flap endonuclease activity. Hot Start Taq DNA Polymerase is chemically modified that leads to complete inactivation of the polymerase until the initial heat activation step at the start of PCR. Hot start PCR reduces non-specific amplification during setup stages of the reaction and helps increase PCR specificity and sensitivity. This product is supplied with a 10x PCR reaction buffer that provides a final concentration of 1.5mM Mg2+ and an optional 5X GC Enhancer that increases the amplification of GC rich templates up to 84%.

Product Specifications

Catalog ID T-511
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T-511-200 200 Units $ 76.00
T-511-1000 1000 Units $ 304.00

Lysozyme, Egg White

There is no image for Lysozyme, Egg White Lysozyme, Egg White
Catalog ID Size Pricing
L-040-1 1 g $ 28.00
L-040-5 5 g $ 44.00
L-040-10 10 g $ 61.00
L-040-25 25 g $ 109.00
L-040-100 100 g $ 218.00
L-040-250 250 g $ 502.00

Description

Lysozyme is an enzyme capable of breaking down the bacterial cell wall, and is typically used to enhance protein extractions or nucleic acid extractions. It works by hydrolyzing the 1,4-β-linkages located between the N-acetylmuramic acid and the N-acetyl-D-glucosamine residues in peptidoglycan.

Gram-positive cells have an increased susceptibility to the actions of lysozyme since gram-positive cells have a higher quantity of peptidoglycan in the cell wall. Gram-negative cells are less vulnerable, but can still be hydrolyzed in the presence of EDTA.

Product Specifications

Catalog ID L-040
CAS # 12650-88-3
Grade MOLECULAR BIOLOGY GRADE
Storage/Handling Store at -20°C.
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L-040-1 1 g $ 28.00
L-040-5 5 g $ 44.00
L-040-10 10 g $ 61.00
L-040-25 25 g $ 109.00
L-040-100 100 g $ 218.00
L-040-250 250 g $ 502.00

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