Enzymes

Enzymes assist in carrying out crucial cellular functions, and GoldBio provides a variety of enzymes to accommodate your research needs, including alkaline phosphatase, DNase, RNase, lysozyme, horseradish peroxidase, pepsin and trypsin among others. These products are offered at a lower rate than other commercially available enzymes without sacrificing quality so that researchers attain the highest value possible.
Showing 1-20 of 29 items.

Alkaline Phosphatase, 2-Component System

There is no image for Alkaline Phosphatase, 2-Component System Alkaline Phosphatase, 2-Component System
Catalog ID Size Pricing
A-440-1 1 KU $ 119.00

Description

Alkaline phosphatase is an enzyme commonly used for the removal of terminal phosphates from the 5' end of DNA or RNA strands to prevent reannealing of the cohesive ends and facilitate future radiolabeling of the strands.

This system includes 30 U/μL of alkaline phosphatase in a 50% glycerol solution and a dilution buffer that allows for the preparation of a 3 U/μL solution for use in DNA modification.

Product Specifications

Catalog ID A-440
Grade BIOTECHNOLOGY GRADE
Storage/Handling store at 4°C. Do NOT freeze.
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A-440-1 1 KU $ 119.00

Cellulase R-10

There is no image for Cellulase R-10 Cellulase R-10
Catalog ID Size Pricing
C8001.0001 1 g $ 93.00
C8001.0005 5 g $ 200.00
C8001.0010 10 g $ 286.00

Description

Cellulase R-10, also called Cellulase "Onozuka" R-10, is derived from Trichoderma Viride. Cellulase R-10 is a multi-enzymatic system consisting of various enzymes, including cellulase, hemicellulase, pectinase and protease. Cellulase R-10 is typically used in association with macerozyme R-10 in the decomposition of plant cell walls to produce protoplasts.

Product Specifications

Catalog ID C8001
CAS # 9012-54-8
Storage/Handling Store at room temperature.
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C8001.0001 1 g $ 93.00
C8001.0005 5 g $ 200.00
C8001.0010 10 g $ 286.00

Cellulase RS

There is no image for Cellulase RS Cellulase RS
Catalog ID Size Pricing
C8003.0001 1 g $ 141.00
C8003.0005 5 g $ 403.00
C8003.0010 10 g $ 636.00

Description

Cellulase “Onozuka RS” is produced by a mutant Trichoderma viride that was derived from the parent strain for Cellulase “Onozuka -R-10”. Cellulase RS contains a very high activity of decomposing natural celluloses. This type of cellulase can be used to obtain protoplasts in a very short time and dissolves cell walls of a wider range of plants.

Product Specifications

Catalog ID C8003
CAS # 9012-54-8
Storage/Handling Store desiccated at 4°C.
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C8003.0001 1 g $ 141.00
C8003.0005 5 g $ 403.00
C8003.0010 10 g $ 636.00

DNase I, Bovine Pancreas (Lysate Tested), >2000 Kunitz U/mg

Catalog ID Size Pricing
D-301-50 50 mg $ 76.00
D-301-100 100 mg $ 111.00
D-301-500 500 mg $ 456.00
D-301-1 1 g $ 869.00

Description

DNase I (Deoxyribonuclease I) is a dialyzed lyophilized powder from bovine pancreas that has been purified by chromatography.

Bovine pancreatic deoxyribonuclease (DNase) is an endonuclease, which splits phosphodiester linkages preferentially adjacent to a pyrimidine nucleotide yielding polynucleotides with a free hydroxyl group at the 3' position and a phosphate group at the 5' position. The optimum pH is 7.8.

DNase I is activated by bivalent metals and inhibited by chelating agents such as EDTA and SDS. DNase I can be stabilized against proteolytic digestion by 5mM Ca2+.

Product Specifications

Catalog ID D-301
CAS # 9003-98-9
Grade MOLECULAR BIOLOGY GRADE
Storage/Handling Store desiccated at -20°C.
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D-301-50 50 mg $ 76.00
D-301-100 100 mg $ 111.00
D-301-500 500 mg $ 456.00
D-301-1 1 g $ 869.00

DNase I, Bovine Pancreas (Lysate Tested), >300 Kunitz U/mg

There is no image for DNase I, Bovine Pancreas (Lysate Tested), >300 Kunitz U/mg DNase I, Bovine Pancreas (Lysate Tested), >300 Kunitz U/mg
Catalog ID Size Pricing
D-303-100 100 mg $ 35.00
D-303-500 500 mg $ 103.00
D-303-1 1 g $ 186.00
D-303-5 5 g $ 751.00

Description

DNase I (Deoxyribonuclease I) is a dialyzed lyophilized powder from bovine pancreas that has been purified by chromatography.

Bovine pancreatic deoxyribonuclease (DNase) is an endonuclease which splits phosphodiester linkages, preferentially adjacent to a pyrimidine nucleotide yielding polynucleotides with a free hydroxyl group at the 3' position and phosphate group at the 5' position. The optimum pH is 7.8.

DNase I is activated by bivalent metals, and inhibited by chelating agents such as EDTA and SDS. DNase I can be stabilized against proteolytic digestion by 5mM Ca 2+.

Product Specifications

Name DNase I
CAS # 9003-98-9
Grade MOLECULAR BIOLOGY GRADE
Storage/Handling Store desiccated at -20°C.
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D-303-100 100 mg $ 35.00
D-303-500 500 mg $ 103.00
D-303-1 1 g $ 186.00
D-303-5 5 g $ 751.00

DNase I, Bovine Pancreas (Lysate Tested), >500 Kunitz U/mg

Catalog ID Size Pricing
D-300-100 100 mg $ 43.00
D-300-500 500 mg $ 126.00
D-300-1 1 g $ 227.00
D-300-5 5 g $ 922.00

Description

DNase I (Deoxyribonuclease I) is a dialyzed lyophilized powder from bovine pancreas that has been purified by chromatography.

Bovine pancreatic deoxyribonuclease (DNase) is an endonuclease which splits phosphodiester linkages, preferentially adjacent to a pyrimidine nucleotide yielding polynucleotides with a free hydroxyl group at the 3' position and phosphate group at the 5' position. The optimum pH is 7.8.

DNase I is activated by bivalent metals, and inhibited by chelating agents such as EDTA and SDS. DNase I can be stabilized against proteolytic digestion by 5 mM Ca2+.

Product Specifications

Catalog ID D-300
CAS # 9003-98-9
Grade MOLECULAR BIOLOGY GRADE
Storage/Handling Store desiccated at -20°C.
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D-300-100 100 mg $ 43.00
D-300-500 500 mg $ 126.00
D-300-1 1 g $ 227.00
D-300-5 5 g $ 922.00

Elastase, Porcine Pancreas

There is no image for Elastase, Porcine Pancreas Elastase, Porcine Pancreas
Catalog ID Size Pricing
E-240-100 100 mg $ 52.00
E-240-500 500 mg $ 85.00
E-240-1 1 g $ 153.00

Description

Elastase is used for tissue dissociation and cell isolation applications. Elastase has an activity of >30 units/mg and is unstable at < pH 3.5. Elastase was purified from a porcine pancreas and then purified by affinity chromatography to remove trace amounts of contaminating proteases. One unit corresponds to the amount of enzyme which liberates 1 µmol 4-nitroaniline per minute at 25°C and pH 7.8.

Product Specifications

Catalog ID E-240
CAS # 39445-21-1
Grade MOLECULAR BIOLOGY GRADE
Storage/Handling Store at -20°C.
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E-240-100 100 mg $ 52.00
E-240-500 500 mg $ 85.00
E-240-1 1 g $ 153.00

Glucose Oxidase >100 U/mg

There is no image for Glucose Oxidase >100 U/mg Glucose Oxidase >100 U/mg
Catalog ID Size Pricing
G-040-50 50 KU $ 41.00
G-040-250 250 KU $ 153.00
G-040-1 1 MU $ 466.00

Description

Glucose oxidase comes from Aspergillus niger and has many applications. Glucose oxidase can be used in the determination of glucose in colorimetric assays in conjunction with peroxidase, in the determination of glucose biosensors or for use as an enzyme label.

Product Specifications

Catalog ID G-040
Grade MOLECULAR BIOLOGY GRADE
Storage/Handling Store at -20°C.
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G-040-50 50 KU $ 41.00
G-040-250 250 KU $ 153.00
G-040-1 1 MU $ 466.00

Glucose Oxidase >225 U/mg

There is no image for Glucose Oxidase >225 U/mg Glucose Oxidase >225 U/mg
Catalog ID Size Pricing
G-041-50 50 KU $ 41.00
G-041-250 250 KU $ 153.00
G-041-1 1 MU $ 466.00

Description

Glucose oxidase comes from Aspergillus niger and has many applications. Glucose oxidase can be used in the determination of glucose in colorimetric assays in conjunction with peroxidase, in the determination of glucose biosensors or for use as en enzyme label.

Product Specifications

Catalog ID G-041
Grade MOLECULAR BIOLOGY GRADE
Storage/Handling Store at -20°C.
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G-041-50 50 KU $ 41.00
G-041-250 250 KU $ 153.00
G-041-1 1 MU $ 466.00

Horseradish peroxidase (HRP)

Catalog ID Size Pricing
P-100-100 100 mg $ 63.00
P-100-250 250 mg $ 117.00
P-100-500 500 mg $ 193.00
P-100-1 1 g $ 331.00
P-100-2.5 2.5 g $ 703.00

Description

Horseradish peroxidase (HRP) is the most commonly used antibody-conjugated enzyme. Substrates for HRP are luminol and benzidine substrates. Tetramethylbenzidine (TMB) and similar substrates are oxidized by HRP in the presence of hydrogen peroxide, or nontoxic substitutes, to yield a chinoid structure of intense blue color.

Product Specifications

Catalog ID P-100
CAS # 9003-99-0
MW ~40,000 g/mol
Grade MOLECULAR BIOLOGY GRADE
Storage/Handling Store at -20°C.
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P-100-100 100 mg $ 63.00
P-100-250 250 mg $ 117.00
P-100-500 500 mg $ 193.00
P-100-1 1 g $ 331.00
P-100-2.5 2.5 g $ 703.00

Hot Start Taq DNA Polymerase

There is no image for Hot Start Taq DNA Polymerase Hot Start Taq DNA Polymerase
Catalog ID Size Pricing
T-510-1000 1000 Units $ 258.00

Description

Hot Start Taq DNA Polymerase is a chemically modified enzyme that remains inactive at room temperature and requires an increase in temperature for activation.

Product Specifications

Catalog ID T-510
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T-510-1000 1000 Units $ 258.00

Hot Start Taq DNA Polymerase plus dNTP

There is no image for Hot Start Taq DNA Polymerase plus dNTP Hot Start Taq DNA Polymerase plus dNTP
Catalog ID Size Pricing
T-511-1000 1000 Units $ 304.00

Description

Hot start Taq is a thermostable DNA polymerase that possesses a 5´→3´ polymerase activity and a 5´ flap endonuclease activity. Hot Start Taq DNA Polymerase is chemically modified that leads to complete inactivation of the polymerase until the initial heat activation step at the start of PCR. Hot start PCR reduces non-specific amplification during setup stages of the reaction and helps increase PCR specificity and sensitivity. This product is supplied with a 10x PCR reaction buffer that provides a final concentration of 1.5mM Mg2+ and an optional 5X GC Enhancer that increases the amplification of GC rich templates up to 84%.

Product Specifications

Catalog ID T-511
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T-511-1000 1000 Units $ 304.00

Lysozyme, Egg White

Catalog ID Size Pricing
L-040-1 1 g $ 23.00
L-040-5 5 g $ 49.00
L-040-10 10 g $ 72.00
L-040-25 25 g $ 126.00
L-040-100 100 g $ 250.00
L-040-250 250 g $ 575.00

Description

Lysozyme is an enzyme capable of breaking down the bacterial cell wall, and is typically used to enhance protein extractions or nucleic acid extractions. It works by hydrolyzing the 1,4-β-linkages located between the N-acetylmuramic acid and the N-acetyl-D-glucosamine residues in peptidoglycan.

Gram-positive cells have an increased susceptibility to the actions of lysozyme since gram-positive cells have a higher quantity of peptidoglycan in the cell wall. Gram-negative cells are less vulnerable, but can still be hydrolyzed in the presence of EDTA.

Product Specifications

Catalog ID L-040
CAS # 12650-88-3
Grade MOLECULAR BIOLOGY GRADE
Storage/Handling Store at -20°C.
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L-040-1 1 g $ 23.00
L-040-5 5 g $ 49.00
L-040-10 10 g $ 72.00
L-040-25 25 g $ 126.00
L-040-100 100 g $ 250.00
L-040-250 250 g $ 575.00

Macerozyme R-10

There is no image for Macerozyme R-10 Macerozyme R-10
Catalog ID Size Pricing
M8002.0001 1 g $ 93.00
M8002.0005 5 g $ 200.00
M8002.0010 10 g $ 286.00

Description

Macerozyme R-10 is a macerating enzyme from the Rhizopus sp. which is perfectly suited for the isolation of plant cells. Macerozyme R-10 is often used in combination with Cellulase "Onozuka R-10" in the isolation of plant protoplasts.

Product Specifications

Catalog ID M8002
CAS # 9032-75-1
Storage/Handling Store at 4°C.
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M8002.0001 1 g $ 93.00
M8002.0005 5 g $ 200.00
M8002.0010 10 g $ 286.00

Pectolyase Y-23

Catalog ID Size Pricing
P8004.0001 1 g $ 415.00
P8004.0005 5 g $ 1,631.00

Description

No description available

Product Specifications

Catalog ID P8004
CAS # 9033-35-6
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P8004.0001 1 g $ 415.00
P8004.0005 5 g $ 1,631.00

Proteinase K Solution 20 mg/ml

Catalog ID Size Pricing
P-480-SL2 2 mL $ 46.00
P-480-SL4 4 mL (2 x 2 mL) $ 74.00
P-480-SL10 10 mL $ 156.00

Description

Proteinase K is a highly reactive nonspecific serine protease that belongs to the subtilisin family of proteins. It cleaves at the carboxylic acid side of aliphatic, aromatic, or hydrophobic amino acids. Proteinase K is capable of inactivating RNases and DNases and is used in the isolation or preparation of high molecular weight nucleic acids. Proteinase K is also useful for helping to characterize enzymes, due to its cleavage specificity. This enzyme was designated proteinase K because of its ability to hydrolyze keratin. Proteinase K is stable in a wide variety of detergents and buffer salts and at various temperatures and pH. The isoelectric point of proteinase K is 8.9.

Product Specifications

Catalog ID P-480-SL
CAS # 39450-01-6
MW 28.5 kDa
Grade MOLECULAR BIOLOGY GRADE
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P-480-SL2 2 mL $ 46.00
P-480-SL4 4 mL (2 x 2 mL) $ 74.00
P-480-SL10 10 mL $ 156.00

Proteinase K, RNase/DNase free

Catalog ID Size Pricing
P-480-100 100 mg $ 54.00
P-480-500 500 mg $ 173.00
P-480-1 1 g $ 215.00
P-480-2 2 x 1 g $ 415.00
P-480-3 3 x 1 g $ 615.00
P-480-4 4 x 1 g $ 810.00
P-480-5 5 x 1 g $ 1,005.00

Description

Proteinase K is a highly reactive nonspecific serine protease that belongs to the subtilisin family of proteins. It cleaves at the carboxylic acid side of aliphatic, aromatic, or hydrophobic amino acids. Proteinase K is capable of inactivating RNases and DNases and is used in the isolation or preparation of high molecular weight nucleic acids. Proteinase K is also useful for helping to characterize enzymes, due to its cleavage specificity. This enzyme was designated proteinase K because of its ability to hydrolyze keratin. Proteinase K is stable in a wide variety of detergents and buffer salts and at various temperatures and pH. The isoelectric point of proteinase K is 8.9.

Product Specifications

Catalog ID P-480
CAS # 39450-01-6
MW 28.5 kDa
Grade MOLECULAR BIOLOGY GRADE
Storage/Handling Store at -20°C.
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P-480-100 100 mg $ 54.00
P-480-500 500 mg $ 173.00
P-480-1 1 g $ 215.00
P-480-2 2 x 1 g $ 415.00
P-480-3 3 x 1 g $ 615.00
P-480-4 4 x 1 g $ 810.00
P-480-5 5 x 1 g $ 1,005.00

RNase A, >70 U/mg

Catalog ID Size Pricing
R-050-50 50 mg $ 47.00
R-050-100 100 mg $ 83.00
R-050-500 500 mg $ 189.00
R-050-1 1 g $ 284.00
R-050-5 5 g $ 1,246.00
R-050-10 10 g $ 2,430.00

Description

RNase A, isolated from bovine pancreas, degrades single-stranded RNA at the C and U residues; therefore, RNase A is pyrimidine specific. Degradation occurs by cleaving the phosphodiester bond located between the 5’-ribose of the nucleotide and the phosphate group that is connected to the 3’-ribose of the neighboring pyrimidine.

Product Specifications

Catalog ID R-050
CAS # 9001-99-4
MW 13.7 kDa
Grade MOLECULAR BIOLOGY GRADE
Storage/Handling Store at -20°C.
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R-050-50 50 mg $ 47.00
R-050-100 100 mg $ 83.00
R-050-500 500 mg $ 189.00
R-050-1 1 g $ 284.00
R-050-5 5 g $ 1,246.00
R-050-10 10 g $ 2,430.00

T4 DNA Ligase - 2000 units/µL

There is no image for T4 DNA Ligase - 2000 units/µL T4 DNA Ligase - 2000 units/µL
Catalog ID Size Pricing
T-411-100 100 KU (2000 units/µl) $ 259.00
T-411-400 400 KU (2000 Units/μl) $ 335.00

Description

T4 DNA Ligase catalyzes the formation of a phosphodiester bond between juxtaposed 5’-phosphate and 3’-hydroxyl termini in duplex DNA or RNA. This enzymes joins DNA fragments with either cohesive or blunt termini as well as repair single stranded nicks in duplex DNA, RNA or DNA/RNA hybrids.

Product Specifications

Catalog ID T-411
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T-411-100 100 KU (2000 units/µl) $ 259.00
T-411-400 400 KU (2000 Units/μl) $ 335.00

T4 DNA Ligase - 400 units/µL

There is no image for T4 DNA Ligase - 400 units/µL T4 DNA Ligase - 400 units/µL
Catalog ID Size Pricing
T-410-100 100 KU (400 Units/μl) $ 259.00

Description

T4 DNA Ligase catalyzes the formation of a phosphodiester bond between juxtaposed 5’-phosphate and 3’-hydroxyl termini in duplex DNA or RNA. This enzymes joins DNA fragments with either cohesive or blunt termini as well as repair single stranded nicks in duplex DNA, RNA or DNA/RNA hybrids.

Product Includes

  • T4 DNA Ligase
  • 10x T4 DNA Ligase Reaction Buffer

Purity: ≥99% (assessed by SDS-PAGE with Coomassie blue staining)

Concentration: 400 unit/μl
One unit is defined as the amount of enzyme required to give 50% ligation of HindIII fragments of λ DNA (250 ng/µl) in a total reaction volume of 20 μl in 30 minutes at 16°C in 1X T4 DNA ligase reaction buffer.

Source: E. coli strain expressing a recombinant clone

Inhibition and Inactivation:
Inhibitors: metal chelators, phosphate and ammonium ions, KCl and NaCl at a concentration higher than 50 mM.
Inactivated by heating at 70°C for 15 min or by addition of EDTA.

Quality Control
Endonuclease Activity (Nicking): 1 µg of supercoiled plasmid DNA is incubated with 20 units of T4 DNA Ligase in 1x Ligase buffer for 2 hours at 37ºC. Following incubation, the supercoiled DNA is visualized on an ethidium bromide stained agarose gel. No visible nicking or cutting of DNA was found.

Product Specifications

Catalog ID T-410
Storage/Handling Store at -20°C.
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T-410-100 100 KU (400 Units/μl) $ 259.00

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