X-Gal, Bluo-Gal, & Magenta-Gal

Galactopyranoside chromogenic substrates, such as X-Gal and its alternatives like Bluo-Gal and Magenta-Gal, are histochemical substrate for β-galactosidase which cleaves the galactopyranoside to yield an insoluble blue (or red in the case of Magenta-Gal) precipitate. These substrates are typically used in conjunction with IPTG for blue-white screening of bacterial cell plate cultures, but are often used in experiments involving β-galactosidase-antibody linked immunoassays and immunohistochemistry. Gold Biotechnology’s substrates are the highest quality on the market but at only a fraction of the price of other suppliers. Our X-Gal is Proven and Published™ to ensure a quality reagent with optimal potential.
Showing 1-3 of 3 items.

Bluo-Gal

Catalog ID Size Pricing
B-673-250 250 mg $ 47.00
B-673-500 500 mg $ 82.00
B-673-1 1 g $ 116.00
B-673-2.5 2.5 g $ 207.00
B-673-10 10 g $ 558.00

Description

Bluo-Gal is used as an alternative to X-Gal, and produces a darker blue color. Bluo-Gal is hydrolyzed by β-galactosidase to produce a blue precipitate. This precipitate is insoluble in alcohol and xylenes, thus making it suitable for immunoblotting and immunocytochemical assays. Bacterial colonies containing active β-galactosidase produce blue colonies when grown on plates containing Bluo-Gal.

Product Specifications

Catalog ID B-673
CAS # 97753-82-7
MW 374.18 g/mol
Grade MOLECULAR BIOLOGY GRADE
Storage/Handling Store at -20°C. Protect from light.
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B-673-250 250 mg $ 47.00
B-673-500 500 mg $ 82.00
B-673-1 1 g $ 116.00
B-673-2.5 2.5 g $ 207.00
B-673-10 10 g $ 558.00

Magenta-Gal

Catalog ID Size Pricing
B-378-50MG 50 mg $ 23.00
B-378-5 5 g $ 219.00
B-378-10 10 g $ 399.00
B-378-25 25 g $ 905.00

Description

No description available

Product Specifications

Catalog ID B-378
CAS # 93863-88-8
MW 408.63 g/mol
Grade MOLECULAR BIOLOGY GRADE
Storage/Handling Store desiccated at -20°C. Protect from light.
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B-378-50MG 50 mg $ 23.00
B-378-5 5 g $ 219.00
B-378-10 10 g $ 399.00
B-378-25 25 g $ 905.00

X-Gal

Catalog ID Size Pricing
X4281C 1 g $ 42.00
X4281C10 10 g $ 229.00

Description

5-Bromo-4-chloro-3-indolyl β-D-galactopyranoside is most commonly known as X-Gal. X-gal is a histochemical substrate for β-galactosidase which cleaves X-Gal to yield an insoluble blue precipitate.

X-Gal is a mainstay in molecular biology cloning applications in which it is used to detect the activity of β-galactosidase. X-Gal is used to detect the insertion of foreign DNA into the lacZ region of a plasmid DNA. Insertion of DNA into the lacZ region results in the loss of β-galactosidase activity. Bacteria cells that retain active β-galactosidase will result in characteristic blue colonies. Successful disruption of the lacZα gene disrupts the α-complementation of the β-galactosidase gene and the precipitate does not form, resulting in white colonies.

Many other applications also use X-Gal as a substrate to detect β-galactosidase activity. These include β-galactosidase -antibody linked immunoassays and immunohistochemistry, coliphage detection based on β-galactosidase induction and the detection of micrometastasis formation during tumor progession.

Product Specifications

Catalog ID X4281
CAS # 7240-90-6
MW 408.63 g/mol
Grade MOLECULAR BIOLOGY GRADE
Storage/Handling Store at -20°C. Protect from light.
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X4281C 1 g $ 42.00
X4281C10 10 g $ 229.00

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