• object(anvilstl\commercio\models\Category)#413 (15) { ["featured_image_urls"]=> array(0) { } ["featured_image"]=> array(0) { } ["banner_image_urls"]=> array(0) { } ["productCount"]=> NULL ["_attributes":"yii\db\BaseActiveRecord":private]=> array(23) { ["id"]=> int(193) ["root"]=> int(1) ["lft"]=> int(374) ["rgt"]=> int(375) ["level"]=> int(1) ["name"]=> string(17) "Featured Products" ["url_name"]=> string(17) "featured-products" ["title"]=> NULL ["short_description"]=> string(0) "" ["long_description"]=> string(283) "

    Our most popular products from our most popular categories, from our Proven and Published™ Luciferin to our popular antibiotic lines and electrophoresis tools. GoldBio’s commitment to quality and affordability makes these products our top sellers.

    " ["hide"]=> int(0) ["third_party_id"]=> NULL ["featured_image_id"]=> int(0) ["banner_image_id"]=> int(0) ["meta_description"]=> string(98) "GoldBio’s most popular products, made to the highest standards and delivered at the lowest cost." ["meta_keywords"]=> string(0) "" ["root_sort"]=> int(0) ["type"]=> string(7) "product" ["condition_type"]=> int(1) ["condition_match_type"]=> int(1) ["sort_type"]=> int(1) ["items_per_page"]=> int(25) ["color"]=> NULL } ["_oldAttributes":"yii\db\BaseActiveRecord":private]=> array(23) { ["id"]=> int(193) ["root"]=> int(1) ["lft"]=> int(374) ["rgt"]=> int(375) ["level"]=> int(1) ["name"]=> string(17) "Featured Products" ["url_name"]=> string(17) "featured-products" ["title"]=> NULL ["short_description"]=> string(0) "" ["long_description"]=> string(283) "

    Our most popular products from our most popular categories, from our Proven and Published™ Luciferin to our popular antibiotic lines and electrophoresis tools. GoldBio’s commitment to quality and affordability makes these products our top sellers.

    " ["hide"]=> int(0) ["third_party_id"]=> NULL ["featured_image_id"]=> int(0) ["banner_image_id"]=> int(0) ["meta_description"]=> string(98) "GoldBio’s most popular products, made to the highest standards and delivered at the lowest cost." 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} [1]=> string(11) "afterInsert" } [1]=> NULL } } ["beforeUpdate"]=> array(1) { [0]=> array(2) { [0]=> array(2) { [0]=> object(creocoder\nestedsets\NestedSetsBehavior)#416 (8) { ["treeAttribute"]=> string(4) "root" ["leftAttribute"]=> string(3) "lft" ["rightAttribute"]=> string(3) "rgt" ["depthAttribute"]=> string(5) "level" ["operation":protected]=> NULL ["node":protected]=> NULL ["owner"]=> *RECURSION* ["_attachedEvents":"yii\base\Behavior":private]=> array(6) { ["beforeInsert"]=> string(12) "beforeInsert" ["afterInsert"]=> string(11) "afterInsert" ["beforeUpdate"]=> string(12) "beforeUpdate" ["afterUpdate"]=> string(11) "afterUpdate" ["beforeDelete"]=> string(12) "beforeDelete" ["afterDelete"]=> string(11) "afterDelete" } } [1]=> string(12) "beforeUpdate" } [1]=> NULL } } ["afterUpdate"]=> array(1) { [0]=> array(2) { [0]=> array(2) { [0]=> object(creocoder\nestedsets\NestedSetsBehavior)#416 (8) { ["treeAttribute"]=> string(4) "root" ["leftAttribute"]=> string(3) "lft" 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NULL } } } ["_eventWildcards":"yii\base\Component":private]=> array(0) { } ["_behaviors":"yii\base\Component":private]=> array(1) { ["tree"]=> object(creocoder\nestedsets\NestedSetsBehavior)#416 (8) { ["treeAttribute"]=> string(4) "root" ["leftAttribute"]=> string(3) "lft" ["rightAttribute"]=> string(3) "rgt" ["depthAttribute"]=> string(5) "level" ["operation":protected]=> NULL ["node":protected]=> NULL ["owner"]=> *RECURSION* ["_attachedEvents":"yii\base\Behavior":private]=> array(6) { ["beforeInsert"]=> string(12) "beforeInsert" ["afterInsert"]=> string(11) "afterInsert" ["beforeUpdate"]=> string(12) "beforeUpdate" ["afterUpdate"]=> string(11) "afterUpdate" ["beforeDelete"]=> string(12) "beforeDelete" ["afterDelete"]=> string(11) "afterDelete" } } } ["__fieldGroups":protected]=> array(0) { } }
  • object(anvilstl\commercio\models\Category)#440 (15) { ["featured_image_urls"]=> array(3) { ["small"]=> string(76) "https://s3.amazonaws.com/commercio/goldbio-2018/collection/small/Buffers.jpg" ["medium"]=> string(77) "https://s3.amazonaws.com/commercio/goldbio-2018/collection/medium/Buffers.jpg" ["large"]=> string(76) "https://s3.amazonaws.com/commercio/goldbio-2018/collection/large/Buffers.jpg" } ["featured_image"]=> array(3) { ["small"]=> string(76) "https://s3.amazonaws.com/commercio/goldbio-2018/collection/small/Buffers.jpg" ["medium"]=> string(77) "https://s3.amazonaws.com/commercio/goldbio-2018/collection/medium/Buffers.jpg" ["large"]=> string(76) "https://s3.amazonaws.com/commercio/goldbio-2018/collection/large/Buffers.jpg" } ["banner_image_urls"]=> array(0) { } ["productCount"]=> NULL ["_attributes":"yii\db\BaseActiveRecord":private]=> array(23) { ["id"]=> int(196) ["root"]=> int(393) ["lft"]=> int(30) ["rgt"]=> int(31) ["level"]=> int(1) ["name"]=> string(13) "Buffers (old)" ["url_name"]=> string(17) "buffers-category2" ["title"]=> NULL ["short_description"]=> string(0) "" ["long_description"]=> string(313) "

    Buffers are essential in many different biological and biotechnical applications. GoldBio’s vast array of application-specific buffers include biological, electrophoretic and lysis buffers in order to ensure that we have exactly the right product for both your research needs as well as your budget.

    " ["hide"]=> int(1) ["third_party_id"]=> NULL ["featured_image_id"]=> int(26) ["banner_image_id"]=> int(0) ["meta_description"]=> string(100) "GoldBio’s large stock of buffers ensures we have a perfect, affordable solution for your research." ["meta_keywords"]=> string(0) "" ["root_sort"]=> int(8) ["type"]=> string(7) "product" ["condition_type"]=> int(1) ["condition_match_type"]=> int(1) ["sort_type"]=> int(1) ["items_per_page"]=> int(25) ["color"]=> string(20) "rgba(255, 64, 87, 1)" } ["_oldAttributes":"yii\db\BaseActiveRecord":private]=> array(23) { ["id"]=> int(196) ["root"]=> int(393) ["lft"]=> int(30) ["rgt"]=> int(31) ["level"]=> int(1) ["name"]=> string(13) "Buffers (old)" ["url_name"]=> string(17) "buffers-category2" ["title"]=> NULL ["short_description"]=> string(0) "" ["long_description"]=> string(313) "

    Buffers are essential in many different biological and biotechnical applications. GoldBio’s vast array of application-specific buffers include biological, electrophoretic and lysis buffers in order to ensure that we have exactly the right product for both your research needs as well as your budget.

    " ["hide"]=> int(1) ["third_party_id"]=> NULL ["featured_image_id"]=> int(26) ["banner_image_id"]=> int(0) ["meta_description"]=> string(100) "GoldBio’s large stock of buffers ensures we have a perfect, affordable solution for your research." 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  • object(anvilstl\commercio\models\Category)#442 (15) { ["featured_image_urls"]=> array(0) { } ["featured_image"]=> array(0) { } ["banner_image_urls"]=> array(0) { } ["productCount"]=> NULL ["_attributes":"yii\db\BaseActiveRecord":private]=> array(23) { ["id"]=> int(224) ["root"]=> int(1) ["lft"]=> int(13) ["rgt"]=> int(14) ["level"]=> int(2) ["name"]=> string(10) "H_Products" ["url_name"]=> string(10) "h-products" ["title"]=> NULL ["short_description"]=> string(0) "" ["long_description"]=> string(0) "" ["hide"]=> int(0) ["third_party_id"]=> NULL ["featured_image_id"]=> int(0) ["banner_image_id"]=> int(0) ["meta_description"]=> string(0) "" ["meta_keywords"]=> string(0) "" ["root_sort"]=> int(0) ["type"]=> string(7) "product" ["condition_type"]=> int(1) ["condition_match_type"]=> int(1) ["sort_type"]=> int(1) ["items_per_page"]=> int(25) ["color"]=> NULL } ["_oldAttributes":"yii\db\BaseActiveRecord":private]=> array(23) { ["id"]=> int(224) ["root"]=> int(1) ["lft"]=> int(13) 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  • object(anvilstl\commercio\models\Category)#444 (15) { ["featured_image_urls"]=> array(0) { } ["featured_image"]=> array(0) { } ["banner_image_urls"]=> array(0) { } ["productCount"]=> NULL ["_attributes":"yii\db\BaseActiveRecord":private]=> array(23) { ["id"]=> int(266) ["root"]=> int(1) ["lft"]=> int(188) ["rgt"]=> int(189) ["level"]=> int(3) ["name"]=> string(18) "Biological Buffers" ["url_name"]=> string(27) "biological-buffers-category" ["title"]=> NULL ["short_description"]=> string(0) "" ["long_description"]=> string(580) "Biological buffers are pH stabilized solutions used for performing electrophoresis, blotting tests, or analyzing cell cultures, and they all have the benefit of being water soluble. Most molecules suitable as buffers can be grouped into one of six different structural families, each with their own range of pH values. GoldBio supplies a wide range of buffers to function with your research condition and pH. 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    Buffers are essential in many different biological and biotechnical applications. GoldBio’s vast array of application-specific buffers include biological, electrophoretic and lysis buffers in order to ensure that we have exactly the right product for both your research needs as well as your budget. Peruse below for our most popular biological buffers! Click here for the comprehensive selection of GoldBio buffers.

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    Buffers are essential in many different biological and biotechnical applications. GoldBio’s vast array of application-specific buffers include biological, electrophoretic and lysis buffers in order to ensure that we have exactly the right product for both your research needs as well as your budget. Peruse below for our most popular biological buffers! Click here for the comprehensive selection of GoldBio buffers.

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    • HEPES, Free Acid

    {{ i.item.name }}
    Specifications Documentation Citations Related Products
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    Product Description

    HEPES is a buffering agent used in biochemistry and molecular biology that was selected and described by Good et al. It is a zwitterionic, piperazinic buffer that is useful for a pH range of 6.8 – 8.2. HEPES lacks the ability to form a significant complex with most metal ions and is recommended for use as a non-coordinating buffer in solutions with metal ions. It is commonly used in cell culture media and as an ampholytic separator to create a pH gradient in isoelectric focusing. HEPES interferes with reactions between DNA and restriction enzymes to a lesser extent than similar buffers with less substituted amine groups, such as Tris. This lack of interference occurs because steric hindrance of the protonated tertiary amine in HEPES shields the negative charge of DNA to a lesser extent than less substituted buffers. This buffer is capable of forming radicals and is therefore not suitable for redox reactions. It is suitable for use in the bicinchoninic acid (BCA) assay.

    HEPES free acid can be mixed with HEPES sodium salt to achieve the desired pH. Alternatively, HEPES free acid can be titrated with NaOH or KOH to achieve the desired pH.

    Product Specifications
    HEPES
    2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonic acid

    ULTRA PURE GRADE

    Formula: C8H18N2O4S

    MW: 238.30 g/mol

    Storage/Handling: Store at room temperature.

    PubChem Chemical ID: 23831

    Product Specifications

    Catalog ID H-400
    CAS # 7365-45-9
    MW 238.30 g/mol
    Grade ULTRA PURE GRADE
    Storage/Handling Store at room temperature.

    Technical Documentation

    P Protocol
    O Other
    C Certificate of Analysis
    S Safety Data Sheet
    P
    HEPES Buffer Stock Solution

    HEPES Stock Solution - 1M
    P
    PHEM Buffer - 4X

    4X PHEM buffer for preserving cell structure
    P
    CaK2-EGTA Stock Solution

    CaK2-EGTA Stock Solution - 100mM
    O
    Printable Buffer Chart - Arranged by Family

    This ultimate buffer selection guide is a fantastic graphical display, showing common buffer pH ranges and their associated pKa. Buffers on this chart are also categorized by family. It's perfect to print out for your lab.
    O
    Printable Buffer pH Chart - Arranged In Order of pH

    Refer to this printable buffer-pH chart for common buffers, associated pKa and pH range. Information on this chart is displayed in vibrant colors, and makes a great print-out for your lab.
    O
    User Guide for GoldBio Buffers

    A user guide for the background, experimental use and technical discussion of GoldBio biological buffers.
    O
    Biological Buffer Selection Guide

    The Biological Buffer Selection Guide includes catalog number, pH range, pKa, applications, stock solution protocol availability and comments for the most common biological buffers.

    C
    Sample COA - H-400

    Sample Certificate of Analysis - HEPES
    COA for example only. Actual tests may differ.
    S
    SDS (MSDS) - HEPES

    Safety Data Sheet

    Citations

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