B_Products

Bis-acrylamide is the most frequently used crosslinking agent for protein separation applications in polyacrylamide gels. Bis-acrylamide powder is freely soluble in water (20 mg/mL) and 2% bis-acrylamide solution is a standard solution used in most electrophoresis applications.

Bis-acrylamide is used to create crosslinks between acrylamide to generate polyacrylamide gel in electrophoresis gels. The ratio of bis-acrylamide to acrylamide manipulates the porous characteristics of the polyacrylamide gel.

Bis-acrylamide is the most frequently used cross-linking agent for protein separation applications in polyacrylamide gels. Bis-acrylamide 2% solution is a standard solution used in most electrophoresis applications. When creating a specialized solution that differs from 2%, use bis-acrylamide powder.

Bis-acrylamide is used to create cross-links between acrylamide to generate polyacrylamide gel in electrophoresis gels. The ratio of bis-acrylamide to acrylamide manipulates the porous characteristics of the polyacrylamide gel.

Bis-Tris, also known as bis-Tris methane, is a zwitterionic buffering agent used in biochemistry and molecular biology. It is a bis(2-hydroxyethyl)amine and Tris family buffer that is useful for a pH range of 5.8 – 7.2. Bis Tris is a common component of many buffer systems for various types of electrophoresis. Bis-Tris may form a complex with some common metals, such as Cu(II) and Pb(II), and formation constants should be taken into account when using this buffer in a solution containing metal ions. Bis-Tris can be used as a substitute for the highly toxic buffer cacodylate.

Bithionol is an anti-parasitic compound used for oncology research and molecular biology research. It induces apoptosis in cancerous cells occurs through activating proteolytic caspases. These caspases inactivate PARP-1(Poly [ADP-ribose] polymerase 1) which triggers an intrinsic pathway for apoptosis. Intrinsic apoptotic pathways result in compromising mitochondria and in this case by decreasing transmembrane potential. Reactive oxygen species then accumulate and rupture the mitochondria, releasing cytochrome c an apoptotic inducer.

Bithionol has been used to better understand enzyme allosteric regulation and the mechanism of soluble adenylyl cyclase inhibition, a bicarbonate sensor. Bithionol has been shown to occupy the bicarbonate binding site on soluble adenylyl cyclase. It has also been utilized in research to elucidate the role of mitochondrial potassium channels in preventing ischemia reperfusion injuries in mice.

GoldBio’s BL21 (DE3) Chemically Competent E. coli cells are high efficiency cells, suitable for a wide variety of applications such as transformation and routine protein expression. BL21 (DE3) chemically competent cells feature a widely used host background, a T7 expression strain, and are deficient in both lon (1) and ompT proteases. In addition, BL21 (DE3) cells are resistant to phage T1 (fhuA2) and are B strain.

Product Specifications
Competent cell type: Chemically Competent
Derivative of: BL21 (DE3)
Species: E. coli
Transformation efficiency: ≥1 x 10⁶ cfu/µg pUC19 DNA
Blue/white screening: Yes

GoldBio’s BL21 (DE3) Electrocompetent E. coli cells are suitable for high efficiency transformation and routine protein expression. Increased cloning efficiencies versus typical BL21 cells makes the BL21 (DE3) Electrocompetent E. coli cells ideal for construction of complex expression libraries and feature ≥1 x 10⁸ cfu/µg efficiency with electroporation. BL21 (DE3) Electrocompetent E. coli cells have a widely used background, feature a T7 expression strain, are deficient in both lon (1) and ompT proteases. These cells are resistant to phage T1(fhuA2) and are B strain.

Product Specifications
Competent cell type: ElectroCompetent
Derivative of: BL21 (DE3)
Species: E. coli
Format: Tubes
Transformation efficiency: ≥1 x 10⁸ cfu/µg pUC19 DNA
Blue/white screening: Yes

GoldBio’s BL21 Chemically Competent E. coli cells are a good, universal strain of cell that is gives great transformation for protein expression and induction, and is especially useful for non-T7 vectors protein expression systems. BL21 chemically competent cells feature a widely used host background, and are deficient in both lon (1) and ompT proteases. In addition, BL21 Chemically Competent E. coli cells are resistant to phage T1 (fhuA2).

Product Specifications
Competent cell type: Chemically Competent
Derivative of: BL21
Species: E. coli
Format: Tubes
Transformation efficiency: ≥1 x 10⁶ cfu/µg pUC19 DNA
Blue/white screening: Yes

Blasticidin S is an aminoacylnucleoside antibiotic isolated from Streptomyces griseochromogenes. It shows high efficacy against both eukaryotic and prokaryotic cells. Aside from its antimicrobial properties, blasticidin has displayed antiviral, antitumor, and antifungal properties and has been used as a selection agent. It has been used to select for cells containing the bls, bsr, or BSD resistance gene as well as HEK293-T cells and HEK-D5 cells. Additionally, blasticidin is effective in preventing cell culture contamination and studying protein synthesis.

Blazin' Blue™ Protein Gel Stain is a ready-to-use protein gel staining solution that is a safer and more effective replacement for traditional Coomassie stains. Blazin' Blue™ offers a faster alternative to protein staining (5-60 minutes) in a single step without the need for fixation or washing. Protein bands can be detected through visible blue staining or by near-infrared fluorescence. And since Blazin' Blue™ is aqueous based, there are no hazardous chemicals like methanol or acetic acid to worry about. Blazin' Blue™ Protein Gel Stain is certified under CCR Title 22 as non-toxic to the environment for drain disposal after a simple pH neutralization step.

Blazin' Bright™ Luminescent Protein Gel Stain is a ready-to-use luminescent protein gel staining solution that is a safer and more effective replacement for traditional fluorescent staining. Blazin' Bright™ offers a faster alternative to protein staining (5-60 minutes) in a single step without the need for fixation (destaining is optional). The Blazin' Bright™ gel stains are aqueous based, there are no hazardous chemicals like methanol or acetic acid to worry about. Blazin' Bright™ Protein Gel Stain is certified under CCR Title 22 as non-toxic to the environment for drain disposal after a simple pH neutralization step.

Blazin' Bright™ has a detection limit around 1-10 ng, depending on the detection method used (staining intensity varies between proteins) and does not cause gel shrinkage. Blazin' Bright™ staining is also fully compatible with mass spectrometry (MS) and Edman-based sequencing.

Blazin' Bright™ Luminescent UV Protein Gel Stain is a ready-to-use luminescent protein gel staining solution that is a safer and more effective replacement for traditional fluorescent staining. Gels stained with our Blazin' Bright™ Luminescent UV Protein Gel Stain can be visualized rapidly (5-30 minutes) in a single step without fixation (destaining is optional). The Blazin' Bright™ gel stains are aqueous based, there are no hazardous chemicals like methanol or acetic acid to worry about. Blazin' Bright™ Protein Gel Stain is certified under CCR Title 22 as non-toxic to the environment for drain disposal after a simple pH neutralization step.

Blazin' Bright™ Luminescent UV has a detection limit around 1-10 ng, depending on the detection method used (staining intensity varies between proteins) and does not cause gel shrinkage. Blazin' Bright™ staining is also fully compatible with mass spectrometry (MS) and Edman-based sequencing.

Bleomycin A5 hydrochloride is a compound part of the glycopeptide antibiotic family. It is currently used in oncology research for its strong stimulatory and inhibitory effects. Bleomycin A5 greatly reduces telomerase activity, hindering the proliferation of malignant cells. It has also been shown to induce in vivo fibrosis and inflammation by generating peroxynitrites, a strong oxidizing agent.

Bleomycin A5 stimulates downstream apoptosis by increasing cysteine-aspartic acid protease-3 (caspase-3) activity, tumor protein 53 (p53) production and transforming growth factor beta 1 (TGF-beta 1) formation. These proteins induce apoptosis directly or indirectly by halting cell growth.

Bleomycin Sulfate is an anti-cancer agent isolated from Streptomyces verticillus. Bleomycin is a mixture of bleomycin A2 and bleomycin B2, in an approximately 2:1 ratio, respectively. However, the ratio varies by lot number.

Bleomycin is used against Hodgkin’s lymphoma. Its mechanism is not well understood; however, it is though that bleomycin chelates metallic ions causing a decrease in enzyme stability. The enzymes react with oxygen and produce free radicals, ultimately causing single-stranded breaks in deoxyribose sugars of DNA.

Blue Tetrazolium chloride is a chemical compound used for sensitive detection of alkaline phosphatase. With immunohistochemistry blue tetrazolium is used as a diagnostic for diseases of the phagocyte function by gauging the resulting blue color in the presence of oxygen formed by the phagocyte while killing bacteria.

The BLUEstain™ 2 Protein ladder is a three-color protein standard with 13 prestained proteins, covering a wide range molecular weights from 3.5 to 245 kDa. Proteins are covalently coupled with a blue chromophore except for two reference bands (one green and one red band at 25 kDa and 75 kDa respectively) when separated on SDS-PAGE (Tris-glycine buffer).

The BLUEstain™ 2 Protein ladder is designed for monitoring protein separation during SDS-polyacrylamide gel electrophoresis, verification of Western transfer efficiency on membranes (PVDF, nylon, or nitrocellulose) and for approximating the size of proteins. The ladder is supplied in gel loading buffer and is ready to use. Do not heat, dilute or add reducing agent before loading.

The BLUEstain™ Protein ladder is a three-color protein standard with 12 prestained proteins covering a wide range molecular weights from ~11 to 245 kDa. Proteins are covalently coupled with a bluechromophore except for two reference bands (one green and one red band at 25 kDa and 75 kDa respectively) when separated on SDS-PAGE (Tris-glycine buffer). The BLUEstain™ Protein ladder, is designed for monitoring protein separation during SDS-polyacrylamide gel electrophoresis, verification of Western transfer efficiency on membranes (PVDF, nylon, or nitrocellulose) and for approximating the size of proteins. The ladder is supplied in gel loading buffer and is ready to use.

Bluo-Gal is used as an alternative to X-Gal, and produces a darker blue color. Bluo-Gal is hydrolyzed by β-galactosidase to produce a blue precipitate. This precipitate is insoluble in alcohol and xylenes, thus making it suitable for immunoblotting and immunocytochemical assays. Bacterial colonies containing active β-galactosidase produce blue colonies when grown on plates containing Bluo-Gal.

Bone Morphogenetic Proteins (BMPs) belong to the TGF-β (Transforming Growth Factor) superfamily of structurally related signaling proteins. BMP2 is a potent osteoinductive cytokine, capable of inducing bone and cartilage formation in association with osteoconductive carriers such as collagen and synthetic hydroxyapatite. In addition, BMP2 plays an important role in cardiac morphogenesis and is expressed in a variety of tissues; including the lung, spleen, brain, liver, prostate, ovary and small intestine. The functional form of BMP2 is a 26kDa protein composed of two identical 115 amino acid polypeptide chains linked by a single disulfide bond.

Sterile Filtered White lyophilized powder. Purified and tested for use in cell culture.

Bone Morphogenetic Proteins (BMPs) belong to the TGF-β (Transforming Growth Factor) superfamily of structurally related signaling proteins. BMP7 is one of at least 15 structurally and functionally related BMPs, originally identified as protein regulators of cartilage and bone formation which have since been shown to be involved in embryogenesis and morphogenesis of various tissues and organs. BMPs have been shown to regulate the growth, differentiation, chemotaxis and apoptosis of a variety of cell types; including mesenchymal cells, epithelial cells, hematopoietic cells and neuronal cells. The active form of BMP7 can consist of a dimer of two identical proteins or a heterodimer of two related BMPs. BMP7 is expressed highest in the kidneys and is genetically linked in mice to eye, skeletal and kidney development.

GoldBio BSA is a highly purified, lyophilized albumin, suitable for Westerns, enzyme systems, protein standards, hybridizations and as a protease-sensitive immunoassays. Reagent Grade, Fatty Acid Free BSA is ideally suited for fatty acid sensitive cell culture systems as well as for the most sensitive diagnostic and research applications.Our BSA is protease and certified TSE free, manufactured in the United States in a closed loop system from USDA-inspected, healthy animals located in the US.