H_Products

Midkine (MDK) is a heparin-binding growth factor that is expressed in various cell types during embryo development and in a variety of carcinomas. It is induced by retinoic acid, as well as during oncogenesis, inflammation, and tissue repair. MDK is pleiotropic and promotes angiogenesis, neurogenesis, chondrogenesis, cell growth, cell migration, and tumorigenesis. It also inhibits apoptosis in some cells. MDK acts through several different receptors including PTP, ALK, PTP-zeta, and LRP. The amino acid sequence of human MDK is about 50% homologous to human pleiotrophin (PTN).

N-gamma-Hydroxy-L-arginine is an intermediate in the biosynthesis of nitric oxide from L-arginine. Arginase is inhibited by N-gamma-Hydroxy-L-arginine, as is the cell proliferation of MDA-MB-468 cancer cells.

Nickel HTC 6% Agarose Beads are suitable for downstream purification using IMAC. These resins combine the advantages of Nickel purification with the high flow rates of the HTC resin (70% higher than commercial standards).

HTC beads are high throughput beads designed to meet the demand for industrial process separation. Their rigidity and mechanical resistance permits high flow rates, with good resolution in a minimum time frame, making these beads ideal for process-scale use. HTC beads are highly crosslinked 4% and 6% agarose matrices and have excellent physical and chromatographic qualities. They are autoclavable. These beads are an ideal support for the immobilization of ligands for Affinity Chromatography and base media support for producing IEX (Ion Exchange) and Hydrophobic interaction chromatography resins.

Nickel NTA HTC Agarose Resin has a binding capacity of ~60 mg/ml. In addition, it allows for purification of proteins under native or denaturing conditions. GoldBio Nickel Agarose works very well with the His-Tag Buffer Set.

NTA cross-linked Agarose resin consists of nitrilotriacetic acid groups ligated by stable ether linkages via a spacer arm. NTA is a tetravalent chelating agent, covalently coupled to cross-linked agarose beads, providing a higher specificity and lower ion leaching than IDA linked resins. NTA resins have also been shown to be more robust in the presence of higher concentrations of EDTA, but may require a higher imidazole concentration for protein elution. This resin is loaded with Ni 2+. The resulting, ready-to-use resin is ideal for rapid purifications of His-tagged proteins.

Noggin belongs to a group of diffusible proteins that bind to ligands of the TGF-β (Transforming Growth Factor) family and regulate their activity by inhibiting their access to signaling receptors. Noggin was originally identified as a BMP4 antagonist whose action is critical for proper formation of the head and other dorsal structures. Noggin has been shown to modulate the activities of other BMPs including BMP2, 7, 13, and 14. Targeted deletion of noggin in mice results in prenatal death and recessive phenotype displaying a severely malformed skeletal system. Transgenic mice over-expressing noggin in mature osteoblasts display impaired osteoblastic differentiation, reduced bone formation and severe osteoporosis.

NOV is a dynamically expressed, multifunctional matricellular protein of the CCN (CTGF/CYR61/NOV) family encoded by the NOV gene. It is expressed in certain tumors, including Wilm’s tumor and most nephroblastomas. Expression of NOV has been reported to reduce tumorgenicity and reduce proliferation of certain cancer cell lines. It has also been shown to induce angiogenesis in endothelial cells and stimulate cell migration in many mesenchymal cell types. Recombinant human NOV is a 36.2 kDa protein containing 331 amino acid residues and it shares 80% and 81% amino acid identity with rat and murine NOV.

Natriuretic Peptide B is synthesized as a 134 residue preprohormone before a signal peptide is cleaved, creating a storage protein which is further cleaved into the 32 residue, biologically active peptide that is secreted into the blood. NPPB was first discovered in porcine brain, but is produced mainly by cardiac ventricle cells in humans. It acts as a cardiac hormone with a variety of functions including natriuresis, diuresis, vasorelaxation, and inhibition of renin and aldosterone secretion. Additionally, it plays a key role in cardiovascular homeostasis, helps restore the body's salt and water balance, and improves heart function. NPPB also has antimicrobial properties against Gram-positive and Gram-negative bacteria as well as yeast in a dose-dependent manner.

Neuregulin 1 belongs to a family of structurally related polypeptide growth factors and is produced in numerous isoforms by alternative splicing, which allows it to perform a wide variety of functions. These isoforms include heregulins (HRGs), glial growth factors (GGFs) and sensory and motor neuron-derived factor (SMDF). They all have the EGF-like domain, and can bind to ErbB3 and ErbB4 receptor tyrosine kinases. This binding induces ErbB3 and ErbB4 heterodimerization with ErbB2, stimulating intrinsic kinase activity. This product is the EGF-like domain of isoform 1. NRG1 isoforms induce the growth and differentiation of epithelial, neuronal, glial, and other types of cells. Isoform 1 induces differentiation of mammary tumor cells, including altered morphology and synthesis of milk components. NRG1 is necessary for cardiac development, structural maintenance, and functional integrity of the heart. It is also thought to play a role in synaptic plasticity. Dysregulation of NRG1 has been linked to various cancers and schizophrenia.

Neuregulin 1 belongs to a family of structurally related polypeptide growth factors and is produced in numerous isoforms by alternative splicing, which allows it to perform a wide variety of functions. These isoforms include heregulins (HRGs), glial growth factors (GGFs) and sensory and motor neuron-derived factor (SMDF). They all have the EGF-like domain, and can bind to ErbB3 and ErbB4 receptor tyrosine kinases. This binding induces ErbB3 and ErbB4 heterodimerization with ErbB2, stimulating intrinsic kinase activity. This recombinant protein is the EGF-like domain (177-241 a.a.) of isoform 6, the major neuronal isoform. NRG1 isoforms induce the growth and differentiation of epithelial, neuronal, glial, and other types of cells. NRG1 is necessary for development, structural maintenance, and functional integrity of the heart. It is also thought to play a role in synaptic plasticity. Dysregulation of NRG1 has been linked to various cancers and schizophrenia.

Neuregulin 1 belongs to a family of structurally related polypeptide growth factors and is produced in numerous isoforms by alternative splicing, which allows it to perform a wide variety of functions. These isoforms include heregulins (HRGs), glial growth factors (GGFs) and sensory and motor neuron-derived factor (SMDF). They all have the EGF-like domain, and can bind to ErbB3 and ErbB4 receptor tyrosine kinases. This binding induces ErbB3 and ErbB4 heterodimerization with ErbB2, stimulating intrinsic kinase activity. This recombinant protein is the EGF-like domain of isoform 7. NRG1 isoforms induce the growth and differentiation of epithelial, neuronal, glial, and other types of cells. NRG1 is necessary for development, structural maintenance, and functional integrity of the heart. It is also thought to play a role in synaptic plasticity. Dysregulation of NRG1 has been linked to various cancers and schizophrenia.

Sterile Filtered White lyophilized powder. Purified and tested for use in cell culture.

Neuritin (NRN1) is a GPI-anchored neurotrophic factor, which is expressed in response to induction of neuronal activity by GDNF, BDNF, NT3 and other neural stimulators. It promotes neurite outgrowth and especially branching of neuritic processes in primary hippocampal and cortical cells. NRN1 also promotes angiogenesis. During early brain development, NRN1 inhibits apoptosis of cortical progenitors by preventing activation of caspase 3. Recombinant human neuritin is a covalently disulfide-linked homodimer, consisting of two 9.7 kDa polypeptide monomers, each containing 88 amino acid residues.

Neurotrophin 4 (NTF4), also known as NT5, is a member of the NGF family of neuronal and epithelial growth factors. Neurotrophins have six conserved cysteine residues that are involved in the formation of three disulfide bonds. Human NT4 shares 48 - 52% amino acid sequence identity with human β-NGF, BDNF and NT3. It shares 91% and 95% amino acid sequence identity with mouse and rat NT4/5, respectively. The protein is secreted as a homodimer and can also form heterodimers with BDNF or NT3. NT4 binds and induces receptor dimerization and activation of TrkB and promotes the development and survival of selected peripheral and CNS neurons. NT4 induced TrkB signaling augments NMDA receptor activity and increases neuronal sensitivity to excitotoxic cell death. NT4 is secreted by activated T cells and granulocytes and contributes to tissue regeneration at inflammation sites.

Platelet-derived growth factor (PDGF) is a glycoprotein present in serum but absent from plasma. PDGF has three subforms: PDGFAA, PDGFBB, and PDGF-AB. This form is a disulfide-linked homodimer consisting of two peptide chains B (PDGFBB). It is involved in a number of biological processes, including hyperplasia, embryonic neuron development, chemotaxis, and respiratory tubule epithelial cell development. In contrast to PDGFAA, the beta chains in PDGFBB are sufficient for mitogenesis. The function of PDGF is mediated by two tyrosine kinase receptors, PDGFR-α and PDGFR-β, both of which bind PDGFBB.

Platelet Factor 4 (PF4) is a non-ELR type of CXC chemokine. PF4 is expressed primarily in megakaryocytes and platelets, but it is also seen in a variety of other cells as well. It is released from alpha granules of activated platelets during platelet aggregation and binds to heparin with high affinity, neutralizing the anticoagulant effect of heparin. Because of this coagulation-promoting activity, it is concluded that PF4 is involved in inflammation and would healing. PF4 binds to the integral membrane protein receptor CXCR3B, an alternatively spliced isoform of CXCR3. It participates in chemotaxis of neutrophils, fibroblasts, and monocytes.

PPBP is an ELR type of CXC chemokine. This recombinant protein is a mature, proteolytically processed form of PPBP containing 70 amino acids that is also known as NAP2. It is expressed in human monocytes after treatment with bacterial endotoxins and released from the alpha-granules of platelets after their activation. NAP2 is chemotactic for fibroblasts and neutrophils. It is involved in a variety of cellular processes including mitogenesis, DNA synthesis, extracellular matrix synthesis, glycolysis, and plasminogen activator synthesis.

Recombinant Protein G contains only IgG binding domains. The albumin-binding domain as well as cell wall and cell membrane binding domains of native Protein G have been removed to ensure the maximum specific IgG binding capacity.

GoldBio offers rProtein G resins to isolate and purify classes, subclasses and fragments of immunoglobulins from cell culture media and biological fluids. Rapid purification and high yield of purified immunoglobulin are obtainable by this method.

Protein G is covalently bound to highly cross-linked agarose beads, providing a very stable bond that can greatly minimize leakage of the Protein G and allowing for reuse of the affinity resin in several purification steps. This resin works well in High Flow conditions.

Persephin (PSPN) belongs to the GDNF ligand subfamily of the TGF-beta superfamily. The amino acid sequence of human PSPN is about 30% analogous to human GDNF. PSPN is synthesized throughout the nervous system and presumably originates from both astroglial cells and neurons. It promotes the survival and growth of mesencephalic dopaminergic and motor neurons. It also plays a role in kidney development by promoting ureteric bud branching. PSPN binds to a receptor complex consisting of Ret tyrosine kinase and GFR-alpha-4.

Human Parathyroid Hormone (PTH) is a hormone expressed and secreted as preproPTH by chief cells of the parathyroid glands when extracellular calcium levels are low. It is coexpressed with furin, the enzyme believed to be responsible for processing proPTH to PTH. PTH is a ligand for two different receptors, PTH1R in bone and kidney and PTH2R found in the CNS, pancreas, and placenta. PTH plays a major role in bone metabolism and calcium homeostasis, as well as renal excretion. Recombinant PTH (1-34) has been used to stimulate bone formation and bone resorption, increasing or decreasing bone mass depending on the mode of administration.

Human Parathyroid Hormone (PTH) is a hormone expressed and secreted as preproPTH by chief cells of the parathyroid glands when extracellular calcium levels are low. It is coexpressed with furin, the enzyme believed to be responsible for processing proPTH to PTH. PTH is a ligand for two different receptors, PTH1R in bone and kidney and PTH2R found in the CNS, pancreas, and placenta. PTH plays a major role in bone metabolism and calcium homeostasis, as well as renal excretion. In osteoblasts, PTH stimulates glycogenesis and the uptake of [1-(14)C]-2-deoxy-D-glucose (2DG).

Sterile Filtered White lyophilized powder. Purified and tested for use in cell culture.

Human Parathyroid Hormone (PTH) is a hormone expressed and secreted as preproPTH by chief cells of the parathyroid glands when extracellular calcium levels are low. It is coexpressed with furin, the enzyme believed to be responsible for processing proPTH to PTH. PTH is secreted as a midregional peptide fragment (7-34) when extracellular calcium levels are high, offsetting the effects of PTH. PTH plays a major role in bone metabolism and calcium homeostasis, as well as renal excretion. PTH (7-34) is a PTH1R antagonist capable of stimulating proliferation and differentiation of hair follicles and epidermis while promoting cell survival by activation of Akt.

GoldBio growth factors are manufactured for RESEARCH USE ONLY and cannot be sold for human consumption!