Immobilized Enzyme Resins

GoldBio's Immobilized Enzyme Resins comprise a convenient method for digestion without the hassle of cleaning out the leftover enzyme after the reaction is finished! These enzymes are linked either to 4% or 6% agarose beads, which makes them easier to recycle and use again, if desired. With immobilized enzymes, the enzymatic rate of the reaction can be adjusted simply by changing the flow through rate and the amount of undesired protein loss through dissolving is also reduced. GoldBio additionally offers a wide variety of affinity purification agarose resins for the downstream applications of your digested products...everything you need for your experiment, at the prices you know are best!

Showing 1-5 of 5 items.

Immobilized Ficin Agarose Resin

Catalog ID Size Pricing
I-010-5 5 mL $ 215.00

Description

Ficin is a cysteine protease enzyme which is derived from the latex of fig trees with activity similar to other cysteine protease enzymes including endopeptidases, aminopeptidases, and dipeptidyl peptidases. Ficin cleaves mouse immunoglobulin G molecules (IgG1) in the hinge region. The concentration of cysteine changes how ficin cleaves IgG1. A F(ab’)2 fragment is formed in the presence of 1mM cysteine. The F(ab’)2 fragments are smaller than whole IgG molecules and better penetrate tissue for antigen recognition while avoiding unspecific binding to Fc receptors. In the presence of 10mM cysteine, two 2 Fab fragments are produced instead. F(ab) fragments block background from primary antibody binding, and block endogenous and exposed immunoglobulins and tissues in labeling experiments.

Immobilized Ficin Agarose Resin offers a convenient solution to the production of F(ab’)2 and Fab fragments while eliminating autolysis and protease contamination and the need to remove the enzymes following digestion. Fab and F(ab’)2 can be further separated from whole IgG with either Protein A Agarose Resin (GoldBio Product # P-400) or ion exchange chromatography.

Activity:1-1.5 mg ficin/ml of resin

Support: 6% Cross-linked Agarose

Storage/Handling: Shipped at ambient temperature. Upon receipt, store at 4°C, do NOT freeze.

Product Specifications

Catalog ID I-010
Storage/Handling store at 4°C. Do NOT freeze.
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I-010-5 5 mL $ 215.00

Immobilized Papain Agarose Resin

Catalog ID Size Pricing
I-020-5 5 mL $ 216.00
I-020-25 25 mL $ 1,023.00

Description

Papain is a cysteine protease enzyme from papaya latex that has a wide variety of activities including endopeptidases, aminopeptidases, dipeptidyl peptidases and enzymes with both exo- and endo-peptidase activity. In particular, papain cleaves immunoglobulin G (IgG) molecules near the hinge region of the antibody, resulting in three similar, ~50 kDa, fragments; an Fc domain and two monovalent Fab domains. This papain-digested antibody is therefore unable to promote agglutination, precipitation, opsonization or lysis. The Fc fragment can then be used to determine the specificity of the Fc receptors without any typical antigen binging interference while the Fab fragments can be used in immunohistochemical studies.

Product Specifications

Catalog ID I-020
Storage/Handling store at 4°C. Do NOT freeze.
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I-020-5 5 mL $ 216.00
I-020-25 25 mL $ 1,023.00

Immobilized Pepsin Agarose Resin

Catalog ID Size Pricing
I-030-5 5 mL $ 196.00

Description

Pepsin, one of the principal digestion enzymes, breaks down proteins in to smaller peptides. It is most efficient in cleaving the peptide bonds between hydrophobic and aromatic amino acids such as phenylalanine, tryptophan and tyrosine. Pepsin readily cleaves immunoglobulin G (IgG) molecules, generating F(ab’)2 fragments and small Fc peptide fragments. The smaller Fc fragments can be easily removed via gel filtration while the F(ab’)2 fragments can then be purified from any remaining, undigested IgG. The F(ab’)2 fragments are highly useful for research interests, being able to penetrate tissue better than standard IgG antibodies and facilitate better antigen recognition while avoiding unspecific binding to Fc receptors.

Immobilized Pepsin Agarose Resin is an efficient method for producing F(ab’)2 fragments from IgG molecules for antibody mimetic. Undigested IgG can be quickly removed with our Protein A Agarose Resin (GoldBio Catalog # P-400). Fc fragments can also be removed using a gel filtration system.

Resin: 6% Cross-linked Agarose

Shipping/Handling: Shipped at ambient temperature. Upon receipt, store at 4°C, do NOT freeze.

Product Specifications

Catalog ID I-030
Storage/Handling store at 4°C. Do NOT freeze.
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I-030-5 5 mL $ 196.00

Immobilized Pepstatin Agarose Resin

Catalog ID Size Pricing
I-040-5 5 mL $ 331.00

Description

Pepstatin A is a competitive, reversible aspartic protease inhibitor which inhibits proteases such as pepsin, chymosin, renin, HIV proteases, and cathepsins D and E. Peptstatin A is highly selective and is often used in a mixture with other enzyme inhibitors. In vitro studies have shown pepstatin A is the only aspartic acid inhibitor that exhibits inhibitory effects on HIV proteases.

Product Specifications

Catalog ID I-040
Storage/Handling store at 4°C. Do NOT freeze.
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I-040-5 5 mL $ 331.00

Immobilized Trypsin Agarose Resin

Catalog ID Size Pricing
I-050-2 2 mL $ 95.00

Description

Trypsin is a serine protease that hydrolyses proteins which can be found in the digestive system. Trypsin cleaves peptide bonds on the carboxy side of s-aminoethyl cysteine, arginine and lysine residues with very little or no cleavage at lysyl-proline and arginyl-proline bonds. This special cleaving activity yields peptides that are easily identified by mass spectrometry. Additionally, our Immobilized Trypsin is TPCK treated to deactivate interfering chymotrypsin, which can interfere with sequence analysis.

Product Specifications

Catalog ID I-050
Storage/Handling store at 4°C. Do NOT freeze.
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I-050-2 2 mL $ 95.00

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