I_Products

IPTG is an analog of galactose that is non-metabolizable and inactivates the lac repressor to induce synthesis of β-galactosidase in E. coli. The expression of cloned genes under the control of the lac operon is induced by IPTG. It is also a substrate for thigalactoside transacetylase and has been reported to induce penicillinase in bacteria.

The IPTG EZ Pak™ is the fastest and easiest way to make a set amount of sterile IPTG solution. The kit includes preweighed IPTG powder, a sterile filter, and a sterile container for the filtered solution. No need to calculate, simply add the stated amount of deionized H₂O, filter, and pour into the labeled bottle for easy usage. The EZ Pak™ includes high quality GoldBio IPTG and the sterile solution is ready for tissue culture, bacterial media, or any other appropriate use.

Irgasan (triclosan) is a broad-spectrum antifungal and antibiotic. It is often used as a supplement to cell culture media in Pseudomonas and Yersinia isolation agars to prevent bacterial contamination. It is slightly soluble in water and freely soluble in ethanol and methanol.

Irgasan binds the enoyl-acyl carrier protein reductase and forms a complex. Consequently, NAD (nicotinamide adenine dinucleotide) binds and prevents the enoyl-ACP complex from participating in the fatty acid synthesis cycle, suppressing bacterial cell growth.

Isocorydine is an alkaloid isolated from plants. Isocorydine induces both PDCD4 and G2M apoptosis in hepatocellular carcinoma and also inhibits eukaryote protein kinases.

Isoniazid is a prodrug derivative of nicotinic acid that is used as an antibiotic compound against Mycobactrium tuberculosis, M. bovis, and other mycobacteria. This prodrug is transformed by catalase-peroxidases, where its active form inhibits an enoyl-reductase (InhA). Inhibition of InhA compromises bacterial cell walls by preventing mycolic acid synthesis, disrupting fluid homeostasis and leading to cell death.

Isoxanthohumol is a prenylflavonoid compound extracted from Humulus lupulus L., the common hop plant. It has displayed strong estrogenic effects and has been used for anti-cancer research. Isoxanthohumol has also been shown to form the potent phytoestrogen 8-prenylnaringenin (8PN), which forms by cleaving off an ether group.

Oncology research on Isoxanthohumol activity has shown a significant degree of efficacy against breast, prostate and colon cancer cell lines. It has also been to shown to attenuate transforming growth factor-β (TGF-1 β) expression and disrupt Janus kinase/ signal transducer and activator of transcription (JAK/STAT) pathways which activate oncogenic genes.

Itraconazole is a triazole antifungal agent used to increase fungal cell membrane permeability by inhibiting ergosterol synthesis, ultimately leading to fungal cell death. Itraconazole is effective against Blastomyces, Histoplasma, and Aspergillus species. It is used to study Candida infections in mice and to study proliferative changes in diabetic rats. Additionally, itraconazole is a hedgehog signaling inhibitor and CYP3A4 inhibitor.

Ixazomib citrate is a citrate salt that is a second generation proteasome inhibitor used for anti-cancer research. In vivo research has shown ixazomib citrate to hydrolyze into its active ixazomib form which reversibly inhibits 20S proteasomes in multiple myeloma cancer cells. Ceasing this enzyme’s activity has been shown to decrease angiogenesis, induce apoptotic pathways and upregulate tumor suppressor genes.

Myo-Inositol is a vitamin added to promote growth in plant cell culture media. It can also be used to differentiate organisms based on metabolic properties. Myo-inositol is a component of membrane phospholipids.

ProBlock™ Gold 2D is a protease inhibitor cocktail specifically developed for sample preparation for IEF/2D- studies. No EDTA is used, which allows optimal action of nuclease activity for removing nucleic acids from the samples.

ProBlock™ Gold 2D contains both irreversible and reversible protease inhibitors and inhibits serine, cysteine and metalloproteases etc. Due to the optimized concentration of the various inhibitors, the ProBlock™ Gold 2D shows excellent inhibition of protease activities and is therefore suitable for the protection of proteins extracted from animal cells / tissues, plants, yeast and bacteria etc.

ProBlock™ Gold 2D at 1X concentration in extraction buffer at pH 7-8 inhibits over 90% of protease activities.

A broad range, 100X concentrated, ready-to-use protease inhibitor cocktail. ProBlock™ Gold Bacterial 2D contains optimized concentrations of various bacterial protease inhibitors, which provide excellent inhibition of protease activities during recombinant protein purification from bacteria. The cocktail contains both reversible and irreversible protease inhibitors to inhibit metallo, serine and cysteine proteases. It also contains specific inhibitors for bacterial proteases, such as aspartic proteases and aminopeptidases. A 1X concentration of Bacterial ProteaseArrest™ inhibits over 90% of protease activities.

ProBlock™ Gold Bacterial 2D cocktail does not use EDTA as its metalloprotease inhibitor as it would inhibit the activity of proteins that require divalent cations (Ca2+, Mg2+, or Mn2+) for their biological activity. In addition, EDTA would inhibit the purification of proteins using immobilized metal affinity chromatography (IMAC), for example His tagged or CBP tagged proteins. ProBlock™ Gold Bacterial 2Dcocktail is compatible with immobilized metal affinity chromatography.

ProBlock™ Gold Bacterial Protease Inhibitor Cocktail contains optimized concentrations of various bacterial protease inhibitors, which provide excellent inhibition of protease activities during protein purification from bacteria. The cocktail contains both reversible and irreversible protease inhibitors to inhibit serine and cysteine proteases. It also contains specific inhibitors for bacterial proteases, such as aspartic proteases and aminopeptidases. A 1X concentration of ProBlock™ Gold Bacterial inhibits over 90% of protease activities.

Some proteins require divalent cations (Ca²⁺, Mg²⁺ or Mn²⁺) for their biological activity and the presence of chelating agents may be detrimental to the protein’s activity. Chelators, such as EDTA, inhibit the purification of proteins by immobilized metal affinity chromatography.

ProBlock™ Gold Bacterial is supplied with additional, optional EDTA and 1,10-Phenanthroline monohydrate which are provided for optimal inhibition of metalloproteases and may be added in the extraction buffer or lysate as needed.

GoldBio’s new ProBlock™ Gold Extra Strength Protease Inhibitor Cocktail is specifically formulated for the most demanding protein purification applications. The optimized concentrations of the various inhibitors in our Extra Strength cocktail deliver an enhanced dose of protease inhibition for the toughest research experiments. ProBlock™ Gold Extra Strength contains both irreversible and reversible protease inhibitors to inhibit serine, cysteine and other proteases.

ProBlock™ Gold mammalian protease inhibitor cocktail contains optimized concentrations of various mammalian protease inhibitors, which provide excellent inhibition of protease activities during protein purification from mammalian cells and tissues. The cocktail contains both reversible and irreversible protease inhibitors to inhibit serine, cysteine and specific inhibitors for mammalian proteases, such as aminopeptidases. A 1X concentration of ProBlock™ Gold Mammalian inhibits over 90% of protease activities.

Some proteins require divalent cations (Ca²⁺, Mg²⁺ or Mn²⁺) for their biological activity and the presence of chelating agents may be detrimental to the protein’s activity. Chelators, such as EDTA, inhibit the purification of proteins by immobilized metal affinity chromatography.

ProBlock™ Gold Mammalian is supplied with additional, optional EDTA and 1,10-Phenanthroline monohydrate which are provided for optimal inhibition of metalloproteases and may be added in the extraction buffer or lysate as needed.

ProBlock™ Gold Plant protease inhibitor cocktail contains optimized concentrations of various plant protease inhibitors, which provide excellent inhibition of protease activities during protein purification from plant. The cocktail contains both reversible and irreversible protease inhibitors to inhibit serine, cysteine, metalloproteases and specific inhibitors for plant proteases, such as aspartic proteases and aminopeptidases. A 1X concentration oft ProBlock™ Gold Plant inhibits over 90% of protease activities.

Due to the optimized concentration of the various inhibitors, the ProBlock™ Gold shows excellent inhibition of protease activities and is therefore suitable for the protection of proteins during purification from animal tissues, plant tissues, yeast and bacteria. ProBlock™ Gold contains both irreversible and reversible protease inhibitors to inhibit serine, cysteine and other proteases. An separate bottle of EDTA is provided to inhibit metalloproteases.

Since some proteins require divalent cations like Ca²⁺, Mg²⁺ or Mn²⁺ for their biological activity, the presence of EDTA may be detrimental to the protein activity. Furthermore, if the protein of interest is purified using immobilized metal chelate affinity chromatography, EDTA must be removed from the buffer before the chromatography. The ProBlock™ Gold is therefore supplied with a separate, optional EDTA solution which may be added to the extraction buffer or lysate as needed.

ProBlock™ Gold Yeast/Fungi protease inhibitor cocktail contains optimized concentrations of various yeast and fungal protease inhibitors, which provide excellent inhibition of protease activities during protein purification from yeast or fungi. The cocktail contains both reversible and irreversible protease inhibitors to inhibit serine, cysteine, metalloproteases and specific inhibitors for yeast or fungal proteases, such as aspartic proteases. At 1X concentration, ProBlock™ Gold Yeast/Fungi inhibits over 90% of protease activities.

Some proteins require divalent cations (Ca²⁺, Mg²⁺ or Mn²⁺) for their biological activity and the presence of chelating agents may be detrimental to the protein’s activity. Chelators, such as EDTA, inhibit the purification of proteins by immobilized metal affinity chromatography.

ProBlock™ Gold Yeast/Fungi is supplied with additional, optional EDTA and 1,10-Phenanthroline monohydrate which are provided for optimal inhibition of metalloproteases and may be added in the extraction buffer or lysate as needed.

ProBlock™-100, EDTA Free is designed for large volumes of extraction/lysis buffers and is supplied as 10 vials per kit, with each vial suitable for 100 ml.

ProBlock-100, EDTA Free protease inhibitor cocktail contains optimized concentrations of various protease inhibitors, which provide excellent inhibition of protease activities during protein purification. ProBlock™ is a superior general protease inhibitor cocktail that is suitable for purification from mammalian, plant, bacteria and yeast samples. The cocktail is EDTA free and contains both irreversible and reversible protease inhibitors to inhibit serine, cysteine and other proteases. At 1X concentration, ProBlock™ inhibits over 90% of protease activities (e.g. Mouse Pancreas Extract 0.5 mg/ml protein).

ProBlock™-100, Plus EDTA is designed for large volumes of extraction/ lysis buffers and is supplied as 10 vials /kit, with each vial suitable for 100 ml.

ProBlock™-100, Plus EDTA protease inhibitor cocktail contains optimized concentrations of various protease inhibitors, which provide excellent inhibition of protease activities during protein purification. ProBlock™ is a superior general protease inhibitor cocktail that is suitable for purification from mammalian, plant, bacteria and yeast samples. The cocktail contains both irreversible and reversible protease inhibitors to inhibit serine, cysteine and other proteases. EDTA is packed separately for inhibiting metalloproteases. At 1X concentration, ProBlock™ inhibits over 90% of protease activities (e.g. Mouse Pancreas Extract 0.5 mg/ml protein).