P_Products

ProBlock™ Gold Yeast/Fungi protease inhibitor cocktail contains optimized concentrations of various yeast and fungal protease inhibitors, which provide excellent inhibition of protease activities during protein purification from yeast or fungi. The cocktail contains both reversible and irreversible protease inhibitors to inhibit serine, cysteine, metalloproteases and specific inhibitors for yeast or fungal proteases, such as aspartic proteases. At 1X concentration, ProBlock™ Gold Yeast/Fungi inhibits over 90% of protease activities.

Some proteins require divalent cations (Ca²⁺, Mg²⁺ or Mn²⁺) for their biological activity and the presence of chelating agents may be detrimental to the protein’s activity. Chelators, such as EDTA, inhibit the purification of proteins by immobilized metal affinity chromatography.

ProBlock™ Gold Yeast/Fungi is supplied with additional, optional EDTA and 1,10-Phenanthroline monohydrate which are provided for optimal inhibition of metalloproteases and may be added in the extraction buffer or lysate as needed.

ProBlock™-100, EDTA Free is designed for large volumes of extraction/lysis buffers and is supplied as 10 vials per kit, with each vial suitable for 100 ml.

ProBlock-100, EDTA Free protease inhibitor cocktail contains optimized concentrations of various protease inhibitors, which provide excellent inhibition of protease activities during protein purification. ProBlock™ is a superior general protease inhibitor cocktail that is suitable for purification from mammalian, plant, bacteria and yeast samples. The cocktail is EDTA free and contains both irreversible and reversible protease inhibitors to inhibit serine, cysteine and other proteases. At 1X concentration, ProBlock™ inhibits over 90% of protease activities (e.g. Mouse Pancreas Extract 0.5 mg/ml protein).

ProBlock™-100, Plus EDTA is designed for large volumes of extraction/ lysis buffers and is supplied as 10 vials /kit, with each vial suitable for 100 ml.

ProBlock™-100, Plus EDTA protease inhibitor cocktail contains optimized concentrations of various protease inhibitors, which provide excellent inhibition of protease activities during protein purification. ProBlock™ is a superior general protease inhibitor cocktail that is suitable for purification from mammalian, plant, bacteria and yeast samples. The cocktail contains both irreversible and reversible protease inhibitors to inhibit serine, cysteine and other proteases. EDTA is packed separately for inhibiting metalloproteases. At 1X concentration, ProBlock™ inhibits over 90% of protease activities (e.g. Mouse Pancreas Extract 0.5 mg/ml protein).

ProBlock™-50, EDTA Free is designed for large volumes of extraction/lysis buffers and is supplied as 1 vial/kit (Cat# GB-326-1), 10 vials /kit (Cat# GB-326-10) or 20 vials/kit (Cat# GB-326-20), with each vial suitable for 50 ml.

ProBlock-50, EDTA Free protease inhibitor cocktail contains optimized concentrations of various protease inhibitors, which provide excellent inhibition of protease activities during protein purification. ProBlock™ is a superior general protease inhibitor cocktail that is suitable for purification from mammalian, plant, bacteria and yeast samples. The cocktail is EDTA free and contains both irreversible and reversible protease inhibitors to inhibit serine, cysteine and other proteases. At 1X concentration, ProBlock™ inhibits over 90% of protease activities (e.g. Mouse Pancreas Extract 0.5 mg/ml protein).

ProBlock™-50, Plus EDTA is designed for large volumes of extraction/lysis buffers and is supplied as 10 vials /kit (Cat# GB-334-10) or 20 vials/kit (Cat# GB-334-20), with each vial suitable for 50 ml sample. EDTA [0.5M, 100X] is also supplied with this kit as an optional metalloprotease inhibitor.

ProBlock™-50, Plus EDTA protease inhibitor cocktail contains optimized concentrations of various protease inhibitors, which provide excellent inhibition of protease activities during protein purification. ProBlock™ is a superior general protease inhibitor cocktail that is suitable for purification from mammalian, plant, bacteria and yeast samples. The cocktail contains both irreversible and reversible protease inhibitors to inhibit serine, cysteine and other proteases. EDTA is packed separately for inhibiting metalloproteases. At 1X concentration, ProBlock™ inhibits over 90% of protease activities (e.g. Mouse Pancreas Extract 0.5 mg/ml protein).

Protein A agarose resin consists of rProtein A covalently bound to cross-linked agarose beads. It provides a very stable bond that can greatly minimize leakage of the Protein A allowing for reuse of the affinity resin in several purification steps. Protein A Agarose resin is available in bulk from 5-100 ml quantities.

Protein A resin is good for the purification of a wide range of Immunoglobulins of different mammalian species and also certain IgG subclasses that have no other affinity (see additional information).

Protein A is a cell wall component of Staphylococcus aureus. It consists of a single polypeptide chain shaped as a cylinder, which contains five antibody–binding domains. These high affinity regions are specifically bonded to Fc region of the immunoglobulins (specially IgGs of different species).

Protein A Magnetic Agarose Beads are designed to provide the easiest and simplest method of monoclonal and polyclonal antibody purification. The antibodies are retained to the magnetic resin which are also covalently conjugated with recombinant Protein A. Magnetic agarose beads provide a convenient and quick method for purification without the need for pipetting or centrifugation. Washing, binding and elution steps will require a magnetic device.

Protein A Magnetic Beads are supplied as a 50% suspension of magnetic beads in 20% ethanol (Each 1000 μl of suspension contains 500 μl of magnetic beads.)

Binding Capacity: ~10 mg human IgG / ml resin.

Recombinant Protein G contains only IgG binding domains. The albumin-binding domain as well as cell wall and cell membrane binding domains of native Protein G have been removed to ensure the maximum specific IgG binding capacity.

GoldBio offers rProtein G resins to isolate and purify classes, subclasses and fragments of immunoglobulins from cell culture media and biological fluids. Rapid purification and high yield of purified immunoglobulin are obtainable by this method.

Protein G is covalently bound to highly cross-linked agarose beads, providing a very stable bond that can greatly minimize leakage of the Protein G and allowing for reuse of the affinity resin in several purification steps. This resin works well in High Flow conditions.

Protein G Magnetic Agarose Beads are designed to provide the easiest and simplest method of monoclonal and polyclonal antibody purification. The antibodies are retained to the magnetic resin which are also covalently conjugated with recombinant Protein G. Magnetic agarose beads provide a convenient and quick method for purification without the need for pipetting or centrifugation. Washing, binding and elution steps will require a magnetic device.

Protein G Magnetic Beads are supplied as a 50% suspension of magnetic beads in 20% ethanol (Each 1000 μl of suspension contains 500 μl of magnetic beads.)

Binding Capacity: ~10 mg human IgG / ml resin.

Protein L Magnetic Agarose Beads are designed to provide the easiest and simplest method of monoclonal and polyclonal antibody purification and immunoprecipitation. The antibodies are retained to the magnetic resin which are also covalently conjugated with recombinant Protein L. Magnetic agarose beads provide a convenient and quick method for purifications of IgG, IgM, IgA, and IgD containing kappa light chains from various species without interfering with the antigen binding site.

Protein L Magnetic Beads are supplied as a 50% suspension of magnetic beads in 20% ethanol (Each 1000 μl of suspension contains 500 μl of magnetic beads.)

Binding Capacity: ~10 mg human IgG / ml resin.

Proteinase K is a highly reactive nonspecific serine protease that belongs to the subtilisin family of proteins. It cleaves at the carboxylic acid side of aliphatic, aromatic, or hydrophobic amino acids. Proteinase K is capable of inactivating RNases and DNases and is used in the isolation or preparation of high molecular weight nucleic acids. Proteinase K is also useful for helping to characterize enzymes, due to its cleavage specificity. This enzyme was designated proteinase K because of its ability to hydrolyze keratin. Proteinase K is stable in a wide variety of detergents and buffer salts and at various temperatures and pH. The isoelectric point of proteinase K is 8.9.

Proteinase K is a highly reactive nonspecific serine protease that belongs to the subtilisin family of proteins. It cleaves at the carboxylic acid side of aliphatic, aromatic, or hydrophobic amino acids. Proteinase K is capable of inactivating RNases and DNases and is used in the isolation or preparation of high molecular weight nucleic acids. Proteinase K is also useful for helping to characterize enzymes, due to its cleavage specificity. This enzyme was designated proteinase K because of its ability to hydrolyze keratin. Proteinase K is stable in a wide variety of detergents and buffer salts and at various temperatures and pH. The isoelectric point of proteinase K is 8.9.

Persephin (PSPN) belongs to the GDNF ligand subfamily of the TGF-beta superfamily. The amino acid sequence of human PSPN is about 30% analogous to human GDNF. PSPN is synthesized throughout the nervous system and presumably originates from both astroglial cells and neurons. It promotes the survival and growth of mesencephalic dopaminergic and motor neurons. It also plays a role in kidney development by promoting ureteric bud branching. PSPN binds to a receptor complex consisting of Ret tyrosine kinase and GFR-alpha-4.

Persephin (PSPN) belongs to the GDNF ligand subfamily of the TGF-beta superfamily. The amino acid sequence of PSPN exhibits significant homology to GDNF. PSPN is synthesized throughout the nervous system and presumably originates from both astroglial cells and neurons. It promotes the survival and growth of mesencephalic dopaminergic and motor neurons. It also plays a role in kidney development by promoting ureteric bud branching. PSPN binds to a receptor complex consisting of Ret tyrosine kinase and GFR-alpha-4.

Human Parathyroid Hormone (PTH) is a hormone expressed and secreted as preproPTH by chief cells of the parathyroid glands when extracellular calcium levels are low. It is coexpressed with furin, the enzyme believed to be responsible for processing proPTH to PTH. PTH is a ligand for two different receptors, PTH1R in bone and kidney and PTH2R found in the CNS, pancreas, and placenta. PTH plays a major role in bone metabolism and calcium homeostasis, as well as renal excretion. Recombinant PTH (1-34) has been used to stimulate bone formation and bone resorption, increasing or decreasing bone mass depending on the mode of administration.

Human Parathyroid Hormone (PTH) is a hormone expressed and secreted as preproPTH by chief cells of the parathyroid glands when extracellular calcium levels are low. It is coexpressed with furin, the enzyme believed to be responsible for processing proPTH to PTH. PTH is a ligand for two different receptors, PTH1R in bone and kidney and PTH2R found in the CNS, pancreas, and placenta. PTH plays a major role in bone metabolism and calcium homeostasis, as well as renal excretion. In osteoblasts, PTH stimulates glycogenesis and the uptake of [1-(14)C]-2-deoxy-D-glucose (2DG).

Sterile Filtered White lyophilized powder. Purified and tested for use in cell culture.

Human Parathyroid Hormone (PTH) is a hormone expressed and secreted as preproPTH by chief cells of the parathyroid glands when extracellular calcium levels are low. It is coexpressed with furin, the enzyme believed to be responsible for processing proPTH to PTH. PTH is secreted as a midregional peptide fragment (7-34) when extracellular calcium levels are high, offsetting the effects of PTH. PTH plays a major role in bone metabolism and calcium homeostasis, as well as renal excretion. PTH (7-34) is a PTH1R antagonist capable of stimulating proliferation and differentiation of hair follicles and epidermis while promoting cell survival by activation of Akt.

GoldBio growth factors are manufactured for RESEARCH USE ONLY and cannot be sold for human consumption!

Human Parathyroid Hormone (PTH) is a hormone expressed and secreted as preproPTH by chief cells of the parathyroid glands when extracellular calcium levels are low. PTH is coexpressed with furin, the enzyme believed to be responsible for processing proPTH to PTH. PTH is secreted as a C-terminal fragment (7-84) when extracellular calcium levels are high, offsetting the effects of PTH. PTH (7-84) acts as an antagonist for PTH1R, as well as a possible carboxy-terminus interacting PTH receptor. PTH and truncated PTH fragments play a major role in bone metabolism and calcium homeostasis, as well as renal excretion. This particular product has been labeled with the stable 15N isotope to help track its activity.

Human Parathyroid Hormone (PTH) is a hormone expressed and secreted as preproPTH by chief cells of the parathyroid glands when extracellular calcium levels are low. PTH is coexpressed with furin, the enzyme believed to be responsible for processing proPTH to PTH. PTH is secreted as a C-terminal fragment (7-84) when extracellular calcium levels are high, offsetting the effects of PTH. PTH (7-84) acts as an antagonist for PTH1R, as well as a possible carboxy-terminus interacting PTH receptor. PTH and truncated PTH fragments play a major role in bone metabolism and calcium homeostasis, as well as renal excretion.

Sterile Filtered White lyophilized powder. Purified and tested for use in cell culture.

Parathyroid hormone (PTH) is a single polypeptide of 84 amino acids. It is a critical hormone in the regulation of Ca2+ homeostasis and is secreted by the parathyroid glands. These glands promote the release of calcium from bone into the blood stream by activating osteoblasts and inhibiting osteoclasts. Secretion of PTH increases when the level of calcium in the extracellular fluid is low. When there is more Ca2+ in the blood, there is increased intestinal absorption of calcium, renal tubular reabsorption of calcium and increased renal excretion of phosphates. Therefore, PTH is a major regulator of bone metabolism.